Tags

Type your tag names separated by a space and hit enter

[Diagnostic value of the anti-IgM SGPG Elisa (Bühlmann laboratories AG) in 147 sera with a monoclonal IgM anti-MAG/SGPG antibody-associated neuropathy].
Ann Biol Clin (Paris). 2007 Jul-Aug; 65(4):369-75.AB

Abstract

Binding of monoclonal IgM antibodies in serum to antigens of the peripheral nervous system such as MAG and SG(L)PG was measured by various non standardised methods. In this study we evaluated a new commercially available IgM anti-SGPG ELISA (Bühlmann Laboratories AG, Switzerland). The results were compared with three different markers and methods: (1) an in-house thin-layer overlay chromatography for IgM reactivity against sulfated glucuronosyl paragloboside (SGPG) antibodies (gold standard), (2) an indirect immunofluorescent assay for detecting IgM antibodies against myelin, and (3) IgM anti-MAG antibodies, a commercially available Kit based on ELISA technology, manufactured by Bühlmann Laboratories AG. 147 patient sera with anti-MAG/SGPG neuropathy and 121 control sera from patients with peripheral neuropathy were analysed. The anti-SGPG autoantibody ELISA turned out to be a very reliable commercially available test with no technical difficulties and both, excellent sensitivity (0.98), and specificity (0.98) for detecting MAG/SGPG antibody-mediated demyelinating neuropathies. Anti-SGPG antibody titers have pratical implications for both, management and follow-up of neuropathies treated with rituximab.

Authors+Show Affiliations

Centre de biologie et pathologie est, Hospices civils de Lyon, Service de neurobiologie, Groupement hospitalier Est, Lyon Bron. christiane.caudie@chu-lyon.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

fre

PubMed ID

17627917

Citation

Caudie, C, et al. "[Diagnostic Value of the anti-IgM SGPG Elisa (Bühlmann Laboratories AG) in 147 Sera With a Monoclonal IgM anti-MAG/SGPG Antibody-associated Neuropathy]." Annales De Biologie Clinique, vol. 65, no. 4, 2007, pp. 369-75.
Caudie C, Bouhour F, Petiot P, et al. [Diagnostic value of the anti-IgM SGPG Elisa (Bühlmann laboratories AG) in 147 sera with a monoclonal IgM anti-MAG/SGPG antibody-associated neuropathy]. Ann Biol Clin (Paris). 2007;65(4):369-75.
Caudie, C., Bouhour, F., Petiot, P., Gonnaud, P. M., Antoine, J. C., & Vial, C. (2007). [Diagnostic value of the anti-IgM SGPG Elisa (Bühlmann laboratories AG) in 147 sera with a monoclonal IgM anti-MAG/SGPG antibody-associated neuropathy]. Annales De Biologie Clinique, 65(4), 369-75.
Caudie C, et al. [Diagnostic Value of the anti-IgM SGPG Elisa (Bühlmann Laboratories AG) in 147 Sera With a Monoclonal IgM anti-MAG/SGPG Antibody-associated Neuropathy]. Ann Biol Clin (Paris). 2007 Jul-Aug;65(4):369-75. PubMed PMID: 17627917.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Diagnostic value of the anti-IgM SGPG Elisa (Bühlmann laboratories AG) in 147 sera with a monoclonal IgM anti-MAG/SGPG antibody-associated neuropathy]. AU - Caudie,C, AU - Bouhour,F, AU - Petiot,P, AU - Gonnaud,P-M, AU - Antoine,J-C, AU - Vial,C, PY - 2007/04/12/received PY - 2007/05/03/accepted PY - 2007/7/14/pubmed PY - 2007/10/17/medline PY - 2007/7/14/entrez SP - 369 EP - 75 JF - Annales de biologie clinique JO - Ann. Biol. Clin. (Paris) VL - 65 IS - 4 N2 - Binding of monoclonal IgM antibodies in serum to antigens of the peripheral nervous system such as MAG and SG(L)PG was measured by various non standardised methods. In this study we evaluated a new commercially available IgM anti-SGPG ELISA (Bühlmann Laboratories AG, Switzerland). The results were compared with three different markers and methods: (1) an in-house thin-layer overlay chromatography for IgM reactivity against sulfated glucuronosyl paragloboside (SGPG) antibodies (gold standard), (2) an indirect immunofluorescent assay for detecting IgM antibodies against myelin, and (3) IgM anti-MAG antibodies, a commercially available Kit based on ELISA technology, manufactured by Bühlmann Laboratories AG. 147 patient sera with anti-MAG/SGPG neuropathy and 121 control sera from patients with peripheral neuropathy were analysed. The anti-SGPG autoantibody ELISA turned out to be a very reliable commercially available test with no technical difficulties and both, excellent sensitivity (0.98), and specificity (0.98) for detecting MAG/SGPG antibody-mediated demyelinating neuropathies. Anti-SGPG antibody titers have pratical implications for both, management and follow-up of neuropathies treated with rituximab. SN - 0003-3898 UR - https://www.unboundmedicine.com/medline/citation/17627917/[Diagnostic_value_of_the_anti_IgM_SGPG_Elisa__Bühlmann_laboratories_AG__in_147_sera_with_a_monoclonal_IgM_anti_MAG/SGPG_antibody_associated_neuropathy]_ L2 - http://www.jle.com/medline.md?issn=0003-3898&vol=65&iss=4&page=369 DB - PRIME DP - Unbound Medicine ER -