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Antifungal activity of Zuccagnia punctata Cav.: evidence for the mechanism of action.
Planta Med. 2007 Aug; 73(10):1074-80.PM

Abstract

Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5 - 250 microg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8 - 16 microg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2',4'-dihydroxy-3'-methoxychalcone (1) and 2',4'-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC (80), MIC (50) and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2',4'-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent.

Authors+Show Affiliations

Farmacognosia, Facultad Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional Rosario, Rosario, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17628836

Citation

Svetaz, Laura, et al. "Antifungal Activity of Zuccagnia Punctata Cav.: Evidence for the Mechanism of Action." Planta Medica, vol. 73, no. 10, 2007, pp. 1074-80.
Svetaz L, Agüero MB, Alvarez S, et al. Antifungal activity of Zuccagnia punctata Cav.: evidence for the mechanism of action. Planta Med. 2007;73(10):1074-80.
Svetaz, L., Agüero, M. B., Alvarez, S., Luna, L., Feresin, G., Derita, M., Tapia, A., & Zacchino, S. (2007). Antifungal activity of Zuccagnia punctata Cav.: evidence for the mechanism of action. Planta Medica, 73(10), 1074-80.
Svetaz L, et al. Antifungal Activity of Zuccagnia Punctata Cav.: Evidence for the Mechanism of Action. Planta Med. 2007;73(10):1074-80. PubMed PMID: 17628836.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antifungal activity of Zuccagnia punctata Cav.: evidence for the mechanism of action. AU - Svetaz,Laura, AU - Agüero,María Belén, AU - Alvarez,Sandra, AU - Luna,Lorena, AU - Feresin,Gabriela, AU - Derita,Marcos, AU - Tapia,Alejandro, AU - Zacchino,Susana, Y1 - 2007/07/12/ PY - 2007/7/14/pubmed PY - 2007/11/7/medline PY - 2007/7/14/entrez SP - 1074 EP - 80 JF - Planta medica JO - Planta Med VL - 73 IS - 10 N2 - Petroleum ether and dichloromethane extracts of fruits, aerial parts and exudate of Zuccagnia punctata Cav. (Fabaceae) showed moderate antifungal activities against the yeasts C. albicans, S. cerevisiae and C. neoformans (MICs: 62.5 - 250 microg/mL) and very strong antifungal activities against the dermatophytes M. gypseum, T. rubrum and T. mentagrophytes (MICs: 8 - 16 microg/mL) thus supporting the ethnopharmacological use of this plant. Antifungal activity-directed fractionation of active extracts by using bioautography led to the isolation of 2',4'-dihydroxy-3'-methoxychalcone (1) and 2',4'-dihydroxychalcone (2) as the compounds responsible for the antifungal activity. Second-order studies included MIC (80), MIC (50) and MFC of both chalcones in an extended panel of clinical isolates of the most sensitive fungi, and also comprised a series of targeted assays. They showed that the most active chalcone 2 is fungicidal rather than fungistatic, does not disrupt the fungal membranes up to 4 x MFC and does not act by inhibiting the fungal cell wall. So, 2',4'-dihydroxychalcone would act by a different mechanism of action than the antifungal drugs in current clinical use, such as amphotericin B, azoles or echinocandins, and thus appears to be very promising as a novel antifungal agent. SN - 0032-0943 UR - https://www.unboundmedicine.com/medline/citation/17628836/Antifungal_activity_of_Zuccagnia_punctata_Cav_:_evidence_for_the_mechanism_of_action_ L2 - http://www.thieme-connect.com/DOI/DOI?10.1055/s-2007-981561 DB - PRIME DP - Unbound Medicine ER -