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Emergence of multidrug-resistant Salmonella enterica serovar Typhi associated with a class 1 integron carrying the dfrA7 gene cassette in Nepal.
Int J Antimicrob Agents. 2007 Oct; 30(4):330-5.IJ

Abstract

A total of 121 Salmonella enterica serovars Typhi and Paratyphi A isolated from enteric fever patients at a university hospital in Nepal between February 2004 and January 2006 were tested for their antimicrobial susceptibility. The occurrence and cassette content of integrons as well as the molecular mechanisms of resistance among the multidrug-resistant (MDR) S. Typhi were evaluated. Thirty-nine percent of the isolates were susceptible to all the antimicrobial agents tested. Seven of the S. Typhi strains were MDR. None of the 121 S. enterica isolates were resistant to ciprofloxacin, cefazolin, rifampicin or kanamycin. All MDR S. Typhi isolates contained a class 1 integron with a single cassette, dfrA7, conferring resistance to trimethoprim. Pulsed-field gel electrophoresis (PFGE) of XbaI-generated genomic restriction fragments yielded 12 different patterns. Five of the seven MDR isolates containing class 1 integrons had an identical PFGE pattern. Resistance to sulfamethoxazole, streptomycin, ampicillin, tetracycline and chloramphenicol was mediated by sul1, strA-strB, blaTEM-like, tetB and catA genes, respectively. To the best of our knowledge, this is the first report of integron-associated multidrug resistance as well as the first molecular characterisation of the mechanism of resistance of S. Typhi isolated from Nepal. This study indicates the spread of integron-associated multidrug resistance in S. Typhi in Nepal.

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Authors+Show Affiliations

Tamang MD
Department of Microbiology, School of Medicine, Kyungpook National University, 101 Dongin-dong-2 ga, Jung-gu, Daegu 700-422, South Korea.
Oh JY
No affiliation info available
Seol SY
No affiliation info available
Kang HY
No affiliation info available
Lee JC
No affiliation info available
Lee YC
No affiliation info available
Cho DT
No affiliation info available
Kim J
No affiliation info available

MeSH

Cluster AnalysisDNA FingerprintingDNA, BacterialDeoxyribonucleases, Type II Site-SpecificDrug Resistance, Multiple, BacterialElectrophoresis, Gel, Pulsed-FieldGenes, BacterialGenotypeHumansIntegronsMicrobial Sensitivity TestsNepalParatyphoid FeverPolymorphism, Restriction Fragment LengthSalmonella paratyphi ASalmonella typhiTyphoid Fever

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17629465

Citation

Tamang, Migma Dorji, et al. "Emergence of Multidrug-resistant Salmonella Enterica Serovar Typhi Associated With a Class 1 Integron Carrying the dfrA7 Gene Cassette in Nepal." International Journal of Antimicrobial Agents, vol. 30, no. 4, 2007, pp. 330-5.
Tamang MD, Oh JY, Seol SY, et al. Emergence of multidrug-resistant Salmonella enterica serovar Typhi associated with a class 1 integron carrying the dfrA7 gene cassette in Nepal. Int J Antimicrob Agents. 2007;30(4):330-5.
Tamang, M. D., Oh, J. Y., Seol, S. Y., Kang, H. Y., Lee, J. C., Lee, Y. C., Cho, D. T., & Kim, J. (2007). Emergence of multidrug-resistant Salmonella enterica serovar Typhi associated with a class 1 integron carrying the dfrA7 gene cassette in Nepal. International Journal of Antimicrobial Agents, 30(4), 330-5.
Tamang MD, et al. Emergence of Multidrug-resistant Salmonella Enterica Serovar Typhi Associated With a Class 1 Integron Carrying the dfrA7 Gene Cassette in Nepal. Int J Antimicrob Agents. 2007;30(4):330-5. PubMed PMID: 17629465.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Emergence of multidrug-resistant Salmonella enterica serovar Typhi associated with a class 1 integron carrying the dfrA7 gene cassette in Nepal. AU - Tamang,Migma Dorji, AU - Oh,Jae Young, AU - Seol,Sung Yong, AU - Kang,Hee Young, AU - Lee,Je Chul, AU - Lee,Yoo Chul, AU - Cho,Dong Taek, AU - Kim,Jungmin, Y1 - 2007/07/12/ PY - 2007/02/05/received PY - 2007/05/03/revised PY - 2007/05/05/accepted PY - 2007/7/17/pubmed PY - 2007/11/14/medline PY - 2007/7/17/entrez SP - 330 EP - 5 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 30 IS - 4 N2 - A total of 121 Salmonella enterica serovars Typhi and Paratyphi A isolated from enteric fever patients at a university hospital in Nepal between February 2004 and January 2006 were tested for their antimicrobial susceptibility. The occurrence and cassette content of integrons as well as the molecular mechanisms of resistance among the multidrug-resistant (MDR) S. Typhi were evaluated. Thirty-nine percent of the isolates were susceptible to all the antimicrobial agents tested. Seven of the S. Typhi strains were MDR. None of the 121 S. enterica isolates were resistant to ciprofloxacin, cefazolin, rifampicin or kanamycin. All MDR S. Typhi isolates contained a class 1 integron with a single cassette, dfrA7, conferring resistance to trimethoprim. Pulsed-field gel electrophoresis (PFGE) of XbaI-generated genomic restriction fragments yielded 12 different patterns. Five of the seven MDR isolates containing class 1 integrons had an identical PFGE pattern. Resistance to sulfamethoxazole, streptomycin, ampicillin, tetracycline and chloramphenicol was mediated by sul1, strA-strB, blaTEM-like, tetB and catA genes, respectively. To the best of our knowledge, this is the first report of integron-associated multidrug resistance as well as the first molecular characterisation of the mechanism of resistance of S. Typhi isolated from Nepal. This study indicates the spread of integron-associated multidrug resistance in S. Typhi in Nepal. SN - 0924-8579 UR - https://www.unboundmedicine.com/medline/citation/17629465/Emergence_of_multidrug_resistant_Salmonella_enterica_serovar_Typhi_associated_with_a_class_1_integron_carrying_the_dfrA7_gene_cassette_in_Nepal_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(07)00254-3 DB - PRIME DP - Unbound Medicine ER -
Grapherence [↓18]

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