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Cooccurring Gentiana verna and Gentiana acaulis and their neighboring plants in two Swiss upper montane meadows harbor distinct arbuscular mycorrhizal fungal communities.
Appl Environ Microbiol. 2007 Sep; 73(17):5426-34.AE

Abstract

The community composition of arbuscular mycorrhizal fungi (AMF) was analyzed in roots of Gentiana verna, Gentiana acaulis, and accompanying plant species from two species-rich Swiss alpine meadows located in the same area. The aim of the study was to elucidate the impact of host preference or host specificity on the AMF community in the roots. The roots were analyzed by nested PCR, restriction fragment length polymorphism screening, and sequencing of ribosomal DNA small-subunit and internal transcribed spacer regions. The AMF sequences were analyzed phylogenetically and used to define monophyletic sequence types. The AMF community composition was strongly influenced by the host plant species, but compositions did not significantly differ between the two sites. Detailed analyses of the two cooccurring gentian species G. verna and G. acaulis, as well as of neighboring Trifolium spp., revealed that their AMF communities differed significantly. All three host plant taxa harbored AMF communities comprising multiple phylotypes from different fungal lineages. A frequent fungal phylotype from Glomus group B was almost exclusively found in Trifolium spp., suggesting some degree of host preference for this fungus in this habitat. In conclusion, the results indicate that within a relatively small area with similar soil and climatic conditions, the host plant species can have a major influence on the AMF communities within the roots. No evidence was found for a narrowing of the mycosymbiont spectrum in the two green gentians, in contrast to previous findings with their achlorophyllous relatives.

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Authors+Show Affiliations

Sykorová Z
Institute of Botany, University of Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland. zuzana.sykorova@unibas.ch
Wiemken A
No affiliation info available
Redecker D
No affiliation info available

MeSH

AltitudeDNA, FungalDNA, Ribosomal SpacerEcosystemFungiGentianaceaeMolecular Sequence DataMycorrhizaePhylogenyPlant RootsPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthSequence Analysis, DNASpecies SpecificitySwitzerlandTrifolium

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17630317

Citation

Sykorová, Zuzana, et al. "Cooccurring Gentiana Verna and Gentiana Acaulis and Their Neighboring Plants in Two Swiss Upper Montane Meadows Harbor Distinct Arbuscular Mycorrhizal Fungal Communities." Applied and Environmental Microbiology, vol. 73, no. 17, 2007, pp. 5426-34.
Sykorová Z, Wiemken A, Redecker D. Cooccurring Gentiana verna and Gentiana acaulis and their neighboring plants in two Swiss upper montane meadows harbor distinct arbuscular mycorrhizal fungal communities. Appl Environ Microbiol. 2007;73(17):5426-34.
Sykorová, Z., Wiemken, A., & Redecker, D. (2007). Cooccurring Gentiana verna and Gentiana acaulis and their neighboring plants in two Swiss upper montane meadows harbor distinct arbuscular mycorrhizal fungal communities. Applied and Environmental Microbiology, 73(17), 5426-34.
Sykorová Z, Wiemken A, Redecker D. Cooccurring Gentiana Verna and Gentiana Acaulis and Their Neighboring Plants in Two Swiss Upper Montane Meadows Harbor Distinct Arbuscular Mycorrhizal Fungal Communities. Appl Environ Microbiol. 2007;73(17):5426-34. PubMed PMID: 17630317.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cooccurring Gentiana verna and Gentiana acaulis and their neighboring plants in two Swiss upper montane meadows harbor distinct arbuscular mycorrhizal fungal communities. AU - Sykorová,Zuzana, AU - Wiemken,Andres, AU - Redecker,Dirk, Y1 - 2007/07/13/ PY - 2007/7/17/pubmed PY - 2007/10/24/medline PY - 2007/7/17/entrez SP - 5426 EP - 34 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 73 IS - 17 N2 - The community composition of arbuscular mycorrhizal fungi (AMF) was analyzed in roots of Gentiana verna, Gentiana acaulis, and accompanying plant species from two species-rich Swiss alpine meadows located in the same area. The aim of the study was to elucidate the impact of host preference or host specificity on the AMF community in the roots. The roots were analyzed by nested PCR, restriction fragment length polymorphism screening, and sequencing of ribosomal DNA small-subunit and internal transcribed spacer regions. The AMF sequences were analyzed phylogenetically and used to define monophyletic sequence types. The AMF community composition was strongly influenced by the host plant species, but compositions did not significantly differ between the two sites. Detailed analyses of the two cooccurring gentian species G. verna and G. acaulis, as well as of neighboring Trifolium spp., revealed that their AMF communities differed significantly. All three host plant taxa harbored AMF communities comprising multiple phylotypes from different fungal lineages. A frequent fungal phylotype from Glomus group B was almost exclusively found in Trifolium spp., suggesting some degree of host preference for this fungus in this habitat. In conclusion, the results indicate that within a relatively small area with similar soil and climatic conditions, the host plant species can have a major influence on the AMF communities within the roots. No evidence was found for a narrowing of the mycosymbiont spectrum in the two green gentians, in contrast to previous findings with their achlorophyllous relatives. SN - 0099-2240 UR - https://www.unboundmedicine.com/medline/citation/17630317/Cooccurring_Gentiana_verna_and_Gentiana_acaulis_and_their_neighboring_plants_in_two_Swiss_upper_montane_meadows_harbor_distinct_arbuscular_mycorrhizal_fungal_communities_ L2 - http://aem.asm.org/cgi/pmidlookup?view=long&pmid=17630317 DB - PRIME DP - Unbound Medicine ER -