Tags

Type your tag names separated by a space and hit enter

Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high on antiretroviral therapy.
AIDS. 2007 Jul 31; 21(12):1525-34.AIDS

Abstract

OBJECTIVE

To examine the relationships between blood CD4 natural regulatory T (Treg) cells, plasma HIV RNA level, CD4 T-cell count and immune activation in untreated HIV-infected patients and immunodeficient patients beginning antiretroviral therapy (ART), using a novel phenotype to define Treg cells (CD25CD127CD4). Data were compared with established Treg cell markers (FoxP3, CTLA-4 and GITR).

METHODS

Twenty-nine untreated HIV-infected patients with CD4 T-cell counts of < 300 or > 400/microl were compared in a cross-sectional study and 12 patients beginning combination ART with < 100 CD4 T cells/mul were followed for 1 year on therapy. Three- and four-colour flow cytometry was used to quantitate proportions of Treg cells.

RESULTS

In control donors and patients with high CD4 T-cell counts, 28-89% (median 60%) of CD25CD127CD4 cells were FoxP3, but < 10% expressed GITR or CTLA-4. Immunodeficient patients also had CD4-negative lymphocytes with the phenotype FoxP3CD127. Proportions of CD25CD127 cells and activated (HLA-DR) cells in the CD4 T-cell population were increased in patients with low CD4 T cell counts. The proportion of CD25CD127CD4 T cells correlated positively with plasma HIV RNA level and CD4 T-cell activation, but inversely with CD4 T-cell count. Longitudinal studies of 12 patients receiving ART in two distinct cohorts (Western Australia and Malaysia) showed that the proportion of CD25CD127CD4 cells decreased slightly over time, but remained above levels seen in non-HIV controls.

CONCLUSIONS

Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high after 1 year on ART.

Authors+Show Affiliations

School of Surgery and Pathology, University of Western Australia, Rear 50 Murray Street, Perth 6000, Western Australia. alyf82@cyllene.uwa.edu.auNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17630546

Citation

Lim, Andrew, et al. "Proportions of Circulating T Cells With a Regulatory Cell Phenotype Increase With HIV-associated Immune Activation and Remain High On Antiretroviral Therapy." AIDS (London, England), vol. 21, no. 12, 2007, pp. 1525-34.
Lim A, Tan D, Price P, et al. Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high on antiretroviral therapy. AIDS. 2007;21(12):1525-34.
Lim, A., Tan, D., Price, P., Kamarulzaman, A., Tan, H. Y., James, I., & French, M. A. (2007). Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high on antiretroviral therapy. AIDS (London, England), 21(12), 1525-34.
Lim A, et al. Proportions of Circulating T Cells With a Regulatory Cell Phenotype Increase With HIV-associated Immune Activation and Remain High On Antiretroviral Therapy. AIDS. 2007 Jul 31;21(12):1525-34. PubMed PMID: 17630546.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high on antiretroviral therapy. AU - Lim,Andrew, AU - Tan,Dino, AU - Price,Patricia, AU - Kamarulzaman,Adeeba, AU - Tan,Hong-Yien, AU - James,Ian, AU - French,Martyn A, PY - 2007/7/17/pubmed PY - 2007/9/29/medline PY - 2007/7/17/entrez SP - 1525 EP - 34 JF - AIDS (London, England) JO - AIDS VL - 21 IS - 12 N2 - OBJECTIVE: To examine the relationships between blood CD4 natural regulatory T (Treg) cells, plasma HIV RNA level, CD4 T-cell count and immune activation in untreated HIV-infected patients and immunodeficient patients beginning antiretroviral therapy (ART), using a novel phenotype to define Treg cells (CD25CD127CD4). Data were compared with established Treg cell markers (FoxP3, CTLA-4 and GITR). METHODS: Twenty-nine untreated HIV-infected patients with CD4 T-cell counts of < 300 or > 400/microl were compared in a cross-sectional study and 12 patients beginning combination ART with < 100 CD4 T cells/mul were followed for 1 year on therapy. Three- and four-colour flow cytometry was used to quantitate proportions of Treg cells. RESULTS: In control donors and patients with high CD4 T-cell counts, 28-89% (median 60%) of CD25CD127CD4 cells were FoxP3, but < 10% expressed GITR or CTLA-4. Immunodeficient patients also had CD4-negative lymphocytes with the phenotype FoxP3CD127. Proportions of CD25CD127 cells and activated (HLA-DR) cells in the CD4 T-cell population were increased in patients with low CD4 T cell counts. The proportion of CD25CD127CD4 T cells correlated positively with plasma HIV RNA level and CD4 T-cell activation, but inversely with CD4 T-cell count. Longitudinal studies of 12 patients receiving ART in two distinct cohorts (Western Australia and Malaysia) showed that the proportion of CD25CD127CD4 cells decreased slightly over time, but remained above levels seen in non-HIV controls. CONCLUSIONS: Proportions of circulating T cells with a regulatory cell phenotype increase with HIV-associated immune activation and remain high after 1 year on ART. SN - 0269-9370 UR - https://www.unboundmedicine.com/medline/citation/17630546/Proportions_of_circulating_T_cells_with_a_regulatory_cell_phenotype_increase_with_HIV_associated_immune_activation_and_remain_high_on_antiretroviral_therapy_ L2 - http://Insights.ovid.com/pubmed?pmid=17630546 DB - PRIME DP - Unbound Medicine ER -