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The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects.

Abstract

BACKGROUND & AIMS

Irritable bowel syndrome (IBS) is a significant gastrointestinal disorder with unknown etiology. The symptoms can greatly weaken patients' quality of life and account for notable economical costs for society. Contribution of the gastrointestinal microbiota in IBS has been suggested. Our objective was to characterize putative differences in gastrointestinal microbiota between patients with IBS and control subjects. These differences could potentially have a causal relationship with the syndrome.

METHODS

Microbial genomes from fecal samples of 24 patients with IBS and 23 controls were collected, pooled in a groupwise manner, and fractionated according to their guanine cytosine content. Selected fractions were analyzed by extensive high-throughput 16S ribosomal RNA gene cloning and sequencing of 3753 clones. Some of the revealed phylogenetic differences were further confirmed by quantitative polymerase chain reaction assays on individual samples.

RESULTS

The coverage of the clone libraries of IBS subtypes and control subjects differed significantly (P < .0253). The samples were also distinguishable by the Bayesian analysis of bacterial population structure. Moreover, significant (P < .05) differences between the clone libraries were found in several bacterial genera, which could be verified by quantitative polymerase chain reaction assays of phylotypes belonging to the genera Coprococcus, Collinsella, and Coprobacillus.

CONCLUSIONS

The study showed that fecal microbiota is significantly altered in IBS. Further studies on molecular mechanisms underlying these alterations are needed to elucidate the exact role of intestinal bacteria in IBS.

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  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Department of Basic Veterinary Sciences, Division of Microbiology and Epidemiology, University of Helsinki, Helsinki, Finland.

    , , , , , , ,

    Source

    Gastroenterology 133:1 2007 Jul pg 24-33

    MeSH

    Adult
    Aged
    Bacteria
    Base Composition
    Bayes Theorem
    Cloning, Molecular
    DNA, Bacterial
    Feces
    Female
    Gene Library
    Humans
    Irritable Bowel Syndrome
    Male
    Middle Aged
    Phylogeny
    RNA, Ribosomal, 16S

    Pub Type(s)

    Journal Article
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    17631127

    Citation

    Kassinen, Anna, et al. "The Fecal Microbiota of Irritable Bowel Syndrome Patients Differs Significantly From That of Healthy Subjects." Gastroenterology, vol. 133, no. 1, 2007, pp. 24-33.
    Kassinen A, Krogius-Kurikka L, Mäkivuokko H, et al. The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterology. 2007;133(1):24-33.
    Kassinen, A., Krogius-Kurikka, L., Mäkivuokko, H., Rinttilä, T., Paulin, L., Corander, J., ... Palva, A. (2007). The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterology, 133(1), pp. 24-33.
    Kassinen A, et al. The Fecal Microbiota of Irritable Bowel Syndrome Patients Differs Significantly From That of Healthy Subjects. Gastroenterology. 2007;133(1):24-33. PubMed PMID: 17631127.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. AU - Kassinen,Anna, AU - Krogius-Kurikka,Lotta, AU - Mäkivuokko,Harri, AU - Rinttilä,Teemu, AU - Paulin,Lars, AU - Corander,Jukka, AU - Malinen,Erja, AU - Apajalahti,Juha, AU - Palva,Airi, Y1 - 2007/04/14/ PY - 2006/09/08/received PY - 2007/03/29/accepted PY - 2007/7/17/pubmed PY - 2007/8/19/medline PY - 2007/7/17/entrez SP - 24 EP - 33 JF - Gastroenterology JO - Gastroenterology VL - 133 IS - 1 N2 - BACKGROUND & AIMS: Irritable bowel syndrome (IBS) is a significant gastrointestinal disorder with unknown etiology. The symptoms can greatly weaken patients' quality of life and account for notable economical costs for society. Contribution of the gastrointestinal microbiota in IBS has been suggested. Our objective was to characterize putative differences in gastrointestinal microbiota between patients with IBS and control subjects. These differences could potentially have a causal relationship with the syndrome. METHODS: Microbial genomes from fecal samples of 24 patients with IBS and 23 controls were collected, pooled in a groupwise manner, and fractionated according to their guanine cytosine content. Selected fractions were analyzed by extensive high-throughput 16S ribosomal RNA gene cloning and sequencing of 3753 clones. Some of the revealed phylogenetic differences were further confirmed by quantitative polymerase chain reaction assays on individual samples. RESULTS: The coverage of the clone libraries of IBS subtypes and control subjects differed significantly (P < .0253). The samples were also distinguishable by the Bayesian analysis of bacterial population structure. Moreover, significant (P < .05) differences between the clone libraries were found in several bacterial genera, which could be verified by quantitative polymerase chain reaction assays of phylotypes belonging to the genera Coprococcus, Collinsella, and Coprobacillus. CONCLUSIONS: The study showed that fecal microbiota is significantly altered in IBS. Further studies on molecular mechanisms underlying these alterations are needed to elucidate the exact role of intestinal bacteria in IBS. SN - 0016-5085 UR - https://www.unboundmedicine.com/medline/citation/17631127/The_fecal_microbiota_of_irritable_bowel_syndrome_patients_differs_significantly_from_that_of_healthy_subjects_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0016-5085(07)00734-2 DB - PRIME DP - Unbound Medicine ER -