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[Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion].
Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007 Jul; 19(7):394-7.ZW

Abstract

OBJECTIVE

To investigate the changes in Toll-like receptor 2/4(TLR2/4) gene expression in liver and lung in ischemia/reperfusion (I/R) injury with or without preconditioning of N-acetyl-L-cysteine (NAC).

METHODS

BALB/c mice were used in a model of partial hepatic I/R injury and randomly divided into sham-injury control group (SH group), hepatic I/R group and hepatic I/R with NAC pretreatment group (NAC group), each n=10. The level of tumor necrosis factor-alpha (TNF-alpha) in portal vein and plasma alanine aminotransferase (ALT) were measured at 1 hour and 3 hours respectively after reperfusion, the expressions of TLR2/4 mRNA and protein in liver and lung were observed with reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting at the same time points.

RESULTS

Compared with I/R group, the expressions of TLR2/4 mRNA and protein in liver and lung in NAC group were decreased at same time points (P<0.05 or P<0.01). The level of TNF-alpha in portal vein and plasma ALT increased continually in I/R group at 1 hour and 3 hours after reperfusion compared with SH group, however, they were significantly lowered in the group pretreated by NAC (P<0.05 or P<0.01).

CONCLUSION

TLR2/4 is activated in liver and lung in the process of partial hepatic I/R injury. NAC can inhibit the activation of TLR2/4 and the induction of TNF-alpha resulted from I/R injury via modulating the state of redox process; thus it might mitigate liver and lung injury following partial hepatic I/R in mice.

Authors+Show Affiliations

Center of Pancreatic Surgery, Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17631703

Citation

Wu, He-shui, et al. "[Protective Effect of N-acetyl-L-cysteine On Liver and Lung Injury in Mice After Partial Hepatic Ischemia/reperfusion]." Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, vol. 19, no. 7, 2007, pp. 394-7.
Wu HS, Jin X, Tian Y, et al. [Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007;19(7):394-7.
Wu, H. S., Jin, X., Tian, Y., Zhang, J. H., & Wang, C. Y. (2007). [Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue = Chinese Critical Care Medicine = Zhongguo Weizhongbing Jijiuyixue, 19(7), 394-7.
Wu HS, et al. [Protective Effect of N-acetyl-L-cysteine On Liver and Lung Injury in Mice After Partial Hepatic Ischemia/reperfusion]. Zhongguo Wei Zhong Bing Ji Jiu Yi Xue. 2007;19(7):394-7. PubMed PMID: 17631703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Protective effect of N-acetyl-L-cysteine on liver and lung injury in mice after partial hepatic ischemia/reperfusion]. AU - Wu,He-shui, AU - Jin,Xin, AU - Tian,Yuan, AU - Zhang,Jing-hui, AU - Wang,Chun-you, PY - 2007/7/17/pubmed PY - 2009/10/9/medline PY - 2007/7/17/entrez SP - 394 EP - 7 JF - Zhongguo wei zhong bing ji jiu yi xue = Chinese critical care medicine = Zhongguo weizhongbing jijiuyixue JO - Zhongguo Wei Zhong Bing Ji Jiu Yi Xue VL - 19 IS - 7 N2 - OBJECTIVE: To investigate the changes in Toll-like receptor 2/4(TLR2/4) gene expression in liver and lung in ischemia/reperfusion (I/R) injury with or without preconditioning of N-acetyl-L-cysteine (NAC). METHODS: BALB/c mice were used in a model of partial hepatic I/R injury and randomly divided into sham-injury control group (SH group), hepatic I/R group and hepatic I/R with NAC pretreatment group (NAC group), each n=10. The level of tumor necrosis factor-alpha (TNF-alpha) in portal vein and plasma alanine aminotransferase (ALT) were measured at 1 hour and 3 hours respectively after reperfusion, the expressions of TLR2/4 mRNA and protein in liver and lung were observed with reverse transcription-polymerase chain reaction (RT-PCR) and Western blotting at the same time points. RESULTS: Compared with I/R group, the expressions of TLR2/4 mRNA and protein in liver and lung in NAC group were decreased at same time points (P<0.05 or P<0.01). The level of TNF-alpha in portal vein and plasma ALT increased continually in I/R group at 1 hour and 3 hours after reperfusion compared with SH group, however, they were significantly lowered in the group pretreated by NAC (P<0.05 or P<0.01). CONCLUSION: TLR2/4 is activated in liver and lung in the process of partial hepatic I/R injury. NAC can inhibit the activation of TLR2/4 and the induction of TNF-alpha resulted from I/R injury via modulating the state of redox process; thus it might mitigate liver and lung injury following partial hepatic I/R in mice. SN - 1003-0603 UR - https://www.unboundmedicine.com/medline/citation/17631703/[Protective_effect_of_N_acetyl_L_cysteine_on_liver_and_lung_injury_in_mice_after_partial_hepatic_ischemia/reperfusion]_ DB - PRIME DP - Unbound Medicine ER -