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Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection.
Anal Biochem. 2007 Oct 15; 369(2):187-91.AB

Abstract

A new analytical method based on capillary electrophoresis (CE) separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed for the determination of octopamine. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of octopamine. The separation and determination of the derivative was performed using a laboratory-built CE system with an optical fiber LED-induced fluorescence detector. Optimal separation was obtained at 20 kV using a background electrolyte solution consisting of 25 mM sodium borate (pH 9.2). High sensitivity detection was achieved by the optical fiber LED-induced fluorescence detection using a purple LED as the excitation source. The limit of detection (signal/noise=3) for octopamine was 5.0 x 10(-9)M. A calibration curve ranging from 1.0 x 10(-8) to 5.0 x 10(-7)M was shown to be linear. Using this method, the levels of octopamine in human plasma from healthy donors were determined.

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin 541004, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17632069

Citation

Yu, Qinglei, et al. "Determination of Octopamine in Human Plasma By Capillary Electrophoresis With Optical Fiber Light-emitting Diode-induced Fluorescence Detection." Analytical Biochemistry, vol. 369, no. 2, 2007, pp. 187-91.
Yu Q, Zhao S, Ye F, et al. Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection. Anal Biochem. 2007;369(2):187-91.
Yu, Q., Zhao, S., Ye, F., & Li, S. (2007). Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection. Analytical Biochemistry, 369(2), 187-91.
Yu Q, et al. Determination of Octopamine in Human Plasma By Capillary Electrophoresis With Optical Fiber Light-emitting Diode-induced Fluorescence Detection. Anal Biochem. 2007 Oct 15;369(2):187-91. PubMed PMID: 17632069.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of octopamine in human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection. AU - Yu,Qinglei, AU - Zhao,Shulin, AU - Ye,Fanggui, AU - Li,Shuting, Y1 - 2007/06/15/ PY - 2007/04/05/received PY - 2007/06/09/revised PY - 2007/06/12/accepted PY - 2007/7/17/pubmed PY - 2007/12/6/medline PY - 2007/7/17/entrez SP - 187 EP - 91 JF - Analytical biochemistry JO - Anal Biochem VL - 369 IS - 2 N2 - A new analytical method based on capillary electrophoresis (CE) separation and optical fiber light-emitting diode (LED)-induced fluorescence detection has been developed for the determination of octopamine. Naphthalene-2,3-dicarboxaldehyde (NDA) was used for precolumn derivatization of octopamine. The separation and determination of the derivative was performed using a laboratory-built CE system with an optical fiber LED-induced fluorescence detector. Optimal separation was obtained at 20 kV using a background electrolyte solution consisting of 25 mM sodium borate (pH 9.2). High sensitivity detection was achieved by the optical fiber LED-induced fluorescence detection using a purple LED as the excitation source. The limit of detection (signal/noise=3) for octopamine was 5.0 x 10(-9)M. A calibration curve ranging from 1.0 x 10(-8) to 5.0 x 10(-7)M was shown to be linear. Using this method, the levels of octopamine in human plasma from healthy donors were determined. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/17632069/Determination_of_octopamine_in_human_plasma_by_capillary_electrophoresis_with_optical_fiber_light_emitting_diode_induced_fluorescence_detection_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(07)00388-0 DB - PRIME DP - Unbound Medicine ER -