Tags

Type your tag names separated by a space and hit enter

Expression of prostaglandin D2 synthase in activated eosinophils in nasal polyps.
Arch Otolaryngol Head Neck Surg. 2007 Jul; 133(7):693-700.AO

Abstract

OBJECTIVE

To clarify the relationship between prostaglandin D2 production and eosinophil accumulation.

DESIGN

Screening and diagnostic tests.

SUBJECTS

Nineteen patients with chronic rhinosinusitis.

INTERVENTIONS

Nasal polyps were obtained from 19 patients at endoscopic sinus surgery. Eosinophils in nasal polyps were counted after hematoxylin-eosin staining and immunostaining with antibodies against 2 eosinophil markers-major basic protein and EG2. Hematopoietic prostaglandin D2 synthase (HPGDS) expression was examined by semiquantitative Western blot analysis and by immunohistochemical staining with anti-HPGDS antibody.

RESULTS

Nasal polyps were divided into 3 groups by the degree of eosinophilic infiltration. Western blot analysis revealed that HPGDS was more intensely and frequently expressed in the group with high infiltration than in the groups with low or medium infiltration. Hematopoietic prostaglandin D2 synthase was immunohistochemically found in a subpopulation of EG2-positive eosinophils that had accumulated in the nasal polyps but not in the EG2-negative resting eosinophils. The ratio of HPGDS-positive eosinophils to EG2-positive eosinophils in the group with high eosinophil infiltration (mean+/-SD, 64.8%+/-19.2%) was twice that in the group with low eosinophil infiltration (30.5%+/-13.8%).

CONCLUSION

Prostaglandin D2 was actively produced by an EG2 and HPGDS double-positive subpopulation of activated eosinophils that had infiltrated into nasal polyps.

Links

Publisher Full Text
Aggregator Full Text

Authors+Show Affiliations

Hyo S
Department of Otorhinolaryngology, Osaka Medical College, Daigaku-cho, Takatsuki City, Osaka 569, Japan. oto039@poh.osaka-med.ac.jp
Kawata R
No affiliation info available
Kadoyama K
No affiliation info available
Eguchi N
No affiliation info available
Kubota T
No affiliation info available
Takenaka H
No affiliation info available
Urade Y
No affiliation info available

MeSH

AdultAgedBlotting, WesternChronic DiseaseEosinophil Granule ProteinsEosinophil Major Basic ProteinEosinophilsFemaleHumansImmunohistochemistryIntramolecular OxidoreductasesLipocalinsMaleMiddle AgedNasal PolypsRhinitisSinusitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17638783

Citation

Hyo, Sawako, et al. "Expression of Prostaglandin D2 Synthase in Activated Eosinophils in Nasal Polyps." Archives of Otolaryngology--head & Neck Surgery, vol. 133, no. 7, 2007, pp. 693-700.
Hyo S, Kawata R, Kadoyama K, et al. Expression of prostaglandin D2 synthase in activated eosinophils in nasal polyps. Arch Otolaryngol Head Neck Surg. 2007;133(7):693-700.
Hyo, S., Kawata, R., Kadoyama, K., Eguchi, N., Kubota, T., Takenaka, H., & Urade, Y. (2007). Expression of prostaglandin D2 synthase in activated eosinophils in nasal polyps. Archives of Otolaryngology--head & Neck Surgery, 133(7), 693-700.
Hyo S, et al. Expression of Prostaglandin D2 Synthase in Activated Eosinophils in Nasal Polyps. Arch Otolaryngol Head Neck Surg. 2007;133(7):693-700. PubMed PMID: 17638783.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of prostaglandin D2 synthase in activated eosinophils in nasal polyps. AU - Hyo,Sawako, AU - Kawata,Ryo, AU - Kadoyama,Keiichi, AU - Eguchi,Naomi, AU - Kubota,Takahiro, AU - Takenaka,Hiroshi, AU - Urade,Yoshihiro, PY - 2007/7/20/pubmed PY - 2007/9/14/medline PY - 2007/7/20/entrez SP - 693 EP - 700 JF - Archives of otolaryngology--head & neck surgery JO - Arch Otolaryngol Head Neck Surg VL - 133 IS - 7 N2 - OBJECTIVE: To clarify the relationship between prostaglandin D2 production and eosinophil accumulation. DESIGN: Screening and diagnostic tests. SUBJECTS: Nineteen patients with chronic rhinosinusitis. INTERVENTIONS: Nasal polyps were obtained from 19 patients at endoscopic sinus surgery. Eosinophils in nasal polyps were counted after hematoxylin-eosin staining and immunostaining with antibodies against 2 eosinophil markers-major basic protein and EG2. Hematopoietic prostaglandin D2 synthase (HPGDS) expression was examined by semiquantitative Western blot analysis and by immunohistochemical staining with anti-HPGDS antibody. RESULTS: Nasal polyps were divided into 3 groups by the degree of eosinophilic infiltration. Western blot analysis revealed that HPGDS was more intensely and frequently expressed in the group with high infiltration than in the groups with low or medium infiltration. Hematopoietic prostaglandin D2 synthase was immunohistochemically found in a subpopulation of EG2-positive eosinophils that had accumulated in the nasal polyps but not in the EG2-negative resting eosinophils. The ratio of HPGDS-positive eosinophils to EG2-positive eosinophils in the group with high eosinophil infiltration (mean+/-SD, 64.8%+/-19.2%) was twice that in the group with low eosinophil infiltration (30.5%+/-13.8%). CONCLUSION: Prostaglandin D2 was actively produced by an EG2 and HPGDS double-positive subpopulation of activated eosinophils that had infiltrated into nasal polyps. SN - 0886-4470 UR - https://www.unboundmedicine.com/medline/citation/17638783/Expression_of_prostaglandin_D2_synthase_in_activated_eosinophils_in_nasal_polyps_ L2 - https://jamanetwork.com/journals/jamaotolaryngology/fullarticle/10.1001/archotol.133.7.693 DB - PRIME DP - Unbound Medicine ER -