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Cannabinoid CB1 receptor stimulation affords neuroprotection in MPTP-induced neurotoxicity by attenuating S100B up-regulation in vitro.
J Mol Med (Berl). 2007 Dec; 85(12):1379-92.JM

Abstract

In this study, we investigated the mechanism of S100B neurotoxicity and the effect of cannabinoids, in C6 cells treated with 1-methyl-4-phenyl 1,2,3,6 tetrahydropyridine (MPTP) and co-cultured with differentiated PC12 cells. MPTP concentration- and time-dependently increased S100B density in C6 cells. This effect was followed by increased C6 cell proliferation and decreased cell viability of co-cultured PC12 cells. An antibody against S100B, given to PC12 cells before co-culture, led to their survival. Treatment with arachidonyl-2-chloroethylamide, a CB1 agonist, significantly inhibited MPTP-induced S100B density in C6 cells and protected co-cultured PC12 cells from cell death. Because MPTP selectively increased the levels of anandamide in C6 cells, the involvement of the endocannabinoid system was investigated by using selective inhibitors of endocannabinoid inactivation (cellular re-uptake or enzymatic hydrolysis) and selective cannabinoid CB1 and CB2 receptor antagonists and by silencing the CB1 receptor. Our data suggest that selective activation of CB1 receptors by either exogenous or endogenous cannabinoids might afford neuroprotection in MPTP-induced neurotoxicity also by controlling S100B up-regulation in activated glial cells.

Authors+Show Affiliations

Department of Experimental Pharmacology, Faculty of Pharmacy, University of Naples Federico II, Via D. Montesano, 49, 80131, Naples, Italy, iuvone@unina.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17639288

Citation

Iuvone, Teresa, et al. "Cannabinoid CB1 Receptor Stimulation Affords Neuroprotection in MPTP-induced Neurotoxicity By Attenuating S100B Up-regulation in Vitro." Journal of Molecular Medicine (Berlin, Germany), vol. 85, no. 12, 2007, pp. 1379-92.
Iuvone T, Esposito G, De Filippis D, et al. Cannabinoid CB1 receptor stimulation affords neuroprotection in MPTP-induced neurotoxicity by attenuating S100B up-regulation in vitro. J Mol Med (Berl). 2007;85(12):1379-92.
Iuvone, T., Esposito, G., De Filippis, D., Bisogno, T., Petrosino, S., Scuderi, C., Di Marzo, V., & Steardo, L. (2007). Cannabinoid CB1 receptor stimulation affords neuroprotection in MPTP-induced neurotoxicity by attenuating S100B up-regulation in vitro. Journal of Molecular Medicine (Berlin, Germany), 85(12), 1379-92.
Iuvone T, et al. Cannabinoid CB1 Receptor Stimulation Affords Neuroprotection in MPTP-induced Neurotoxicity By Attenuating S100B Up-regulation in Vitro. J Mol Med (Berl). 2007;85(12):1379-92. PubMed PMID: 17639288.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid CB1 receptor stimulation affords neuroprotection in MPTP-induced neurotoxicity by attenuating S100B up-regulation in vitro. AU - Iuvone,Teresa, AU - Esposito,Giuseppe, AU - De Filippis,Daniele, AU - Bisogno,Tiziana, AU - Petrosino,Stefania, AU - Scuderi,Caterina, AU - Di Marzo,Vincenzo, AU - Steardo,Luca, AU - ,, Y1 - 2007/07/17/ PY - 2006/12/01/received PY - 2007/05/22/accepted PY - 2007/03/30/revised PY - 2007/7/20/pubmed PY - 2008/3/14/medline PY - 2007/7/20/entrez SP - 1379 EP - 92 JF - Journal of molecular medicine (Berlin, Germany) JO - J Mol Med (Berl) VL - 85 IS - 12 N2 - In this study, we investigated the mechanism of S100B neurotoxicity and the effect of cannabinoids, in C6 cells treated with 1-methyl-4-phenyl 1,2,3,6 tetrahydropyridine (MPTP) and co-cultured with differentiated PC12 cells. MPTP concentration- and time-dependently increased S100B density in C6 cells. This effect was followed by increased C6 cell proliferation and decreased cell viability of co-cultured PC12 cells. An antibody against S100B, given to PC12 cells before co-culture, led to their survival. Treatment with arachidonyl-2-chloroethylamide, a CB1 agonist, significantly inhibited MPTP-induced S100B density in C6 cells and protected co-cultured PC12 cells from cell death. Because MPTP selectively increased the levels of anandamide in C6 cells, the involvement of the endocannabinoid system was investigated by using selective inhibitors of endocannabinoid inactivation (cellular re-uptake or enzymatic hydrolysis) and selective cannabinoid CB1 and CB2 receptor antagonists and by silencing the CB1 receptor. Our data suggest that selective activation of CB1 receptors by either exogenous or endogenous cannabinoids might afford neuroprotection in MPTP-induced neurotoxicity also by controlling S100B up-regulation in activated glial cells. SN - 0946-2716 UR - https://www.unboundmedicine.com/medline/citation/17639288/Cannabinoid_CB1_receptor_stimulation_affords_neuroprotection_in_MPTP_induced_neurotoxicity_by_attenuating_S100B_up_regulation_in_vitro_ L2 - http://amigo.geneontology.org/amigo/reference/PMID:17639288 DB - PRIME DP - Unbound Medicine ER -