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[Effects of Fuzheng Huayu recipe on MMP-2 activity and type IV collagen expression at fibrotic lung].
Zhongguo Zhong Yao Za Zhi. 2007 May; 32(9):835-9.ZZ

Abstract

OBJECTIVE

To study the mechanism of Fuzheng Huayu (FZHY) recipe against pulmonary fibrosis relating to MMP-2 activity and type IV collagen expression at lung tissue.

METHOD

The pulmonary fibrosis model was induced by intratracheal instillation with bleomycin once in rats. The models were divided into 3 groups: model control, FZHY recipe treated, and methylprednisolone (Solu-Medrol) treated group, each group was of 14 model rats. Normal control group with 10 rats was intoxicated with the same amount of saline. From the second day of intoxication, FZHY recipe treated group orally took FZHY recipe at the dosage of 4.6 g x kg(-1) rat wt, methylprednisolone treated group received intraperitoneal injection with 15 mg x kg(-1) rat wt of methylprednisolone, while model and normal controls took the same volume of saline, 1 time each day and lasting for 4 weeks. Lung and body weights were weighed and the lung/body ratio was calculated. Collagens deposition was check with Masson stain, and lung hydroxyproline (Hyp) content was assayed with Jamall's method. Protein expressions of MMP-2/9 and type IV collagen at lung tissue were analyzed with Western blot and of MMP-2/9 activities by gelatin zymography.

RESULT

Compared to normal rats, the model control rats had a high lung/body ratio, remarkable collagen deposition, increased Hyp content and the expressions of type IV collagen, MMP-2 and MMP-9 protein at lung tissue, increased MMP-2 activity, in particular active MMP-2 activity, but decreased MMP-9 activity. Compared to model control, FZHY recipe and methylprednisolone obviously attenuated pulmonary collagen deposition, decreased lung/body ratio and Hyp content, downregulated MMP-2 protein expression and activity, in particular active MMP-2 activity, and FZHU recipe had some better actions than methylprednisolone on lung/body ratio, type IV collagen expression and active MMP-2 activity. But both drug groups had no influence on MMP-9 protein expression and activity.

CONCLUSION

FZHY recipe has a good action against experimental pulmonary fibrosis and its mechanisms are associated with the inhibition of MMP-2 protein and activity, and with the inhibition of over expression of type IV collagen at lung tissue.

Authors+Show Affiliations

Shuguang Hospitol, Shanghai University of Traditional Chinese Medicine, Shanghai 200021, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

17639988

Citation

Tan, Shan-Zhong, et al. "[Effects of Fuzheng Huayu Recipe On MMP-2 Activity and Type IV Collagen Expression at Fibrotic Lung]." Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, vol. 32, no. 9, 2007, pp. 835-9.
Tan SZ, Liu CH, Zhang W, et al. [Effects of Fuzheng Huayu recipe on MMP-2 activity and type IV collagen expression at fibrotic lung]. Zhongguo Zhong Yao Za Zhi. 2007;32(9):835-9.
Tan, S. Z., Liu, C. H., Zhang, W., Lu, X., Ye, W. C., Cai, Z. Z., & Liu, P. (2007). [Effects of Fuzheng Huayu recipe on MMP-2 activity and type IV collagen expression at fibrotic lung]. Zhongguo Zhong Yao Za Zhi = Zhongguo Zhongyao Zazhi = China Journal of Chinese Materia Medica, 32(9), 835-9.
Tan SZ, et al. [Effects of Fuzheng Huayu Recipe On MMP-2 Activity and Type IV Collagen Expression at Fibrotic Lung]. Zhongguo Zhong Yao Za Zhi. 2007;32(9):835-9. PubMed PMID: 17639988.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of Fuzheng Huayu recipe on MMP-2 activity and type IV collagen expression at fibrotic lung]. AU - Tan,Shan-Zhong, AU - Liu,Cheng-Hai, AU - Zhang,Wei, AU - Lu,Xiong, AU - Ye,Wei-Cheng, AU - Cai,Zhen-Zhen, AU - Liu,Ping, PY - 2007/7/21/pubmed PY - 2008/7/18/medline PY - 2007/7/21/entrez SP - 835 EP - 9 JF - Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica JO - Zhongguo Zhong Yao Za Zhi VL - 32 IS - 9 N2 - OBJECTIVE: To study the mechanism of Fuzheng Huayu (FZHY) recipe against pulmonary fibrosis relating to MMP-2 activity and type IV collagen expression at lung tissue. METHOD: The pulmonary fibrosis model was induced by intratracheal instillation with bleomycin once in rats. The models were divided into 3 groups: model control, FZHY recipe treated, and methylprednisolone (Solu-Medrol) treated group, each group was of 14 model rats. Normal control group with 10 rats was intoxicated with the same amount of saline. From the second day of intoxication, FZHY recipe treated group orally took FZHY recipe at the dosage of 4.6 g x kg(-1) rat wt, methylprednisolone treated group received intraperitoneal injection with 15 mg x kg(-1) rat wt of methylprednisolone, while model and normal controls took the same volume of saline, 1 time each day and lasting for 4 weeks. Lung and body weights were weighed and the lung/body ratio was calculated. Collagens deposition was check with Masson stain, and lung hydroxyproline (Hyp) content was assayed with Jamall's method. Protein expressions of MMP-2/9 and type IV collagen at lung tissue were analyzed with Western blot and of MMP-2/9 activities by gelatin zymography. RESULT: Compared to normal rats, the model control rats had a high lung/body ratio, remarkable collagen deposition, increased Hyp content and the expressions of type IV collagen, MMP-2 and MMP-9 protein at lung tissue, increased MMP-2 activity, in particular active MMP-2 activity, but decreased MMP-9 activity. Compared to model control, FZHY recipe and methylprednisolone obviously attenuated pulmonary collagen deposition, decreased lung/body ratio and Hyp content, downregulated MMP-2 protein expression and activity, in particular active MMP-2 activity, and FZHU recipe had some better actions than methylprednisolone on lung/body ratio, type IV collagen expression and active MMP-2 activity. But both drug groups had no influence on MMP-9 protein expression and activity. CONCLUSION: FZHY recipe has a good action against experimental pulmonary fibrosis and its mechanisms are associated with the inhibition of MMP-2 protein and activity, and with the inhibition of over expression of type IV collagen at lung tissue. SN - 1001-5302 UR - https://www.unboundmedicine.com/medline/citation/17639988/[Effects_of_Fuzheng_Huayu_recipe_on_MMP_2_activity_and_type_IV_collagen_expression_at_fibrotic_lung]_ L2 - https://medlineplus.gov/pulmonaryfibrosis.html DB - PRIME DP - Unbound Medicine ER -