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Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk.
Rapid Commun Mass Spectrom. 2007; 21(16):2693-703.RC

Abstract

A procedure based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) is described for the determination of nicotine and its principal metabolites cotinine, trans-3-hydroxycotinine and cotinine-N-oxide, caffeine and arecoline in breast milk, using N-ethylnorcotinine as internal standard. Liquid/liquid extraction with chloroform/isopropanol (95:5, v/v) was used for nicotine, cotinine, trans-3-hydroxycotinine, cotinine-N-oxide and caffeine under neutral conditions and for arecoline under basic conditions. Chromatography was performed on a C(8) reversed-phase column using a gradient of 50 mM ammonium formate, pH 5.0, and acetonitrile as a mobile phase at a flow rate of 0.5 mL/min. Separated analytes were determined by electrospray ionization tandem mass spectrometry in the positive ion mode using multiple reaction monitoring. Limits of quantification were 5 microg/L for nicotine, cotinine, trans-3-hydroxycotinine, cotinine-N-oxide and caffeine, and 50 microg/L for arecoline using 1 mL human milk per assay. Calibration curves were linear over the calibration ranges for all the substances under investigation, with a minimum r(2) > 0.998. At three concentrations spanning the linear dynamic range of the assay, mean recoveries from breast milk ranged between 71.8 and 77.4% for different analytes. This method was applied to the analysis of analytes in human milk to assess substance exposure in breast-fed infants in relation to eventual clinical outcomes. This LC/MS/MS assay provides adequate sensitivity and performance characteristics for the simultaneous quantification of biomarkers of three of the drugs most commonly used worldwide (tobacco, caffeine and areca nut).

Authors+Show Affiliations

Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanitá, Rome, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17640086

Citation

Pellegrini, Manuela, et al. "Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites, Caffeine and Arecoline in Breast Milk." Rapid Communications in Mass Spectrometry : RCM, vol. 21, no. 16, 2007, pp. 2693-703.
Pellegrini M, Marchei E, Rossi S, et al. Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk. Rapid Commun Mass Spectrom. 2007;21(16):2693-703.
Pellegrini, M., Marchei, E., Rossi, S., Vagnarelli, F., Durgbanshi, A., García-Algar, O., Vall, O., & Pichini, S. (2007). Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk. Rapid Communications in Mass Spectrometry : RCM, 21(16), 2693-703.
Pellegrini M, et al. Liquid Chromatography/electrospray Ionization Tandem Mass Spectrometry Assay for Determination of Nicotine and Metabolites, Caffeine and Arecoline in Breast Milk. Rapid Commun Mass Spectrom. 2007;21(16):2693-703. PubMed PMID: 17640086.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Liquid chromatography/electrospray ionization tandem mass spectrometry assay for determination of nicotine and metabolites, caffeine and arecoline in breast milk. AU - Pellegrini,Manuela, AU - Marchei,Emilia, AU - Rossi,Silvia, AU - Vagnarelli,Federica, AU - Durgbanshi,Abhilasha, AU - García-Algar,Oscar, AU - Vall,Oriol, AU - Pichini,Simona, PY - 2007/7/21/pubmed PY - 2007/9/26/medline PY - 2007/7/21/entrez SP - 2693 EP - 703 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 21 IS - 16 N2 - A procedure based on liquid chromatography/tandem mass spectrometry (LC/MS/MS) is described for the determination of nicotine and its principal metabolites cotinine, trans-3-hydroxycotinine and cotinine-N-oxide, caffeine and arecoline in breast milk, using N-ethylnorcotinine as internal standard. Liquid/liquid extraction with chloroform/isopropanol (95:5, v/v) was used for nicotine, cotinine, trans-3-hydroxycotinine, cotinine-N-oxide and caffeine under neutral conditions and for arecoline under basic conditions. Chromatography was performed on a C(8) reversed-phase column using a gradient of 50 mM ammonium formate, pH 5.0, and acetonitrile as a mobile phase at a flow rate of 0.5 mL/min. Separated analytes were determined by electrospray ionization tandem mass spectrometry in the positive ion mode using multiple reaction monitoring. Limits of quantification were 5 microg/L for nicotine, cotinine, trans-3-hydroxycotinine, cotinine-N-oxide and caffeine, and 50 microg/L for arecoline using 1 mL human milk per assay. Calibration curves were linear over the calibration ranges for all the substances under investigation, with a minimum r(2) > 0.998. At three concentrations spanning the linear dynamic range of the assay, mean recoveries from breast milk ranged between 71.8 and 77.4% for different analytes. This method was applied to the analysis of analytes in human milk to assess substance exposure in breast-fed infants in relation to eventual clinical outcomes. This LC/MS/MS assay provides adequate sensitivity and performance characteristics for the simultaneous quantification of biomarkers of three of the drugs most commonly used worldwide (tobacco, caffeine and areca nut). SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/17640086/Liquid_chromatography/electrospray_ionization_tandem_mass_spectrometry_assay_for_determination_of_nicotine_and_metabolites_caffeine_and_arecoline_in_breast_milk_ L2 - https://doi.org/10.1002/rcm.3137 DB - PRIME DP - Unbound Medicine ER -