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Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats.
Biochem Biophys Res Commun. 1991 Dec 31; 181(3):1028-34.BB

Abstract

In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), an activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when rats were administered [14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function.

Authors+Show Affiliations

Morotomi M
Department of Surgery, Columbia-Presbyterian Medical Center Columbia University, New York, NY 10032.
LoGerfo P
No affiliation info available
Weinstein IB
No affiliation info available

MeSH

AnimalsBiological TransportCarbon RadioisotopesColonDigestive SystemDiglyceridesFecesFood AnalysisIntestinal AbsorptionIntestinal MucosaKineticsMaleRatsRats, Inbred F344Time Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

1764053

Citation

Morotomi, M, et al. "Fecal Excretion, Uptake and Metabolism By Colon Mucosa of Diacylglycerol in Rats." Biochemical and Biophysical Research Communications, vol. 181, no. 3, 1991, pp. 1028-34.
Morotomi M, LoGerfo P, Weinstein IB. Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats. Biochem Biophys Res Commun. 1991;181(3):1028-34.
Morotomi, M., LoGerfo, P., & Weinstein, I. B. (1991). Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats. Biochemical and Biophysical Research Communications, 181(3), 1028-34.
Morotomi M, LoGerfo P, Weinstein IB. Fecal Excretion, Uptake and Metabolism By Colon Mucosa of Diacylglycerol in Rats. Biochem Biophys Res Commun. 1991 Dec 31;181(3):1028-34. PubMed PMID: 1764053.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fecal excretion, uptake and metabolism by colon mucosa of diacylglycerol in rats. AU - Morotomi,M, AU - LoGerfo,P, AU - Weinstein,I B, PY - 1991/12/31/pubmed PY - 1991/12/31/medline PY - 1991/12/31/entrez SP - 1028 EP - 34 JF - Biochemical and biophysical research communications JO - Biochem Biophys Res Commun VL - 181 IS - 3 N2 - In a previous paper we demonstrated that human fecal bacteria can convert phosphatidylcholine to diacylglycerol (DAG), an activator of protein kinase C. The present study demonstrates that several foods contain appreciable levels of DAG, especially certain vegetable oils. On the other hand, when rats were administered [14C]-labeled DAG by intragastric intubation less than 0.1% of the administered radioactivity was recovered as DAG in the feces. Thus only negligible amounts of dietary DAG actually reach the colon. When [14C]DAG was injected directly into ligated segments of rat colon we found appreciable uptake of the intact DAG by the mucosal cells. The major metabolite was arachidonic acid, suggesting that the DAG lipase pathway is more active than the DAG kinase pathway in these cells. Taken together, these results are consistent with our hypothesis that much of the DAG present in the colonic lumen is produced by the intestinal bacteria and that this DAG can actually enter the colonic mucosal cells, where it might influence their function. SN - 0006-291X UR - https://www.unboundmedicine.com/medline/citation/1764053/Fecal_excretion_uptake_and_metabolism_by_colon_mucosa_of_diacylglycerol_in_rats_ DB - PRIME DP - Unbound Medicine ER -