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Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters.
Jpn J Infect Dis. 2007 Jul; 60(4):230-4.JJ

Abstract

An indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (LPS) of Burkholderia pseudomallei and taxonomically closely related species was developed with the Luminex system. LPSs of Pseudomonas aeruginosa, Burkholderia cepacia, Burkholderia thailandensis, Burkholderia vietnamiensis, B. pseudomallei, and Burkholderia mallei were successfully conjugated to Luminex microspheres. Antibodies measured against the LPS of B. pseudomallei-conjugated Luminex beads only cross-reacted with those of two genetically closely related species, B. mallei and B. thailandensis (previously classified as non-pathogenic arabinose-negative B. pseudomallei). However, this system could distinguish other closely related species from B. pseudomallei. This assay is able to detect significantly high levels of anti-LPS antibodies of B. pseudomallei in serum from patients with culture-proven melioidosis.

Authors+Show Affiliations

Department of Microbiology, Regeneration and Advanced Medical Sciency, Gifu University Graduate School of Medicine, Gifu 501-1194, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17642542

Citation

Iihara, Hirotoshi, et al. "Rapid Multiplex Immunofluorescent Assay to Detect Antibodies Against Burkholderia Pseudomallei and Taxonomically Closely Related Nonfermenters." Japanese Journal of Infectious Diseases, vol. 60, no. 4, 2007, pp. 230-4.
Iihara H, Niwa T, Shah MM, et al. Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters. Jpn J Infect Dis. 2007;60(4):230-4.
Iihara, H., Niwa, T., Shah, M. M., Nhung, P. H., Song, S. X., Hayashi, M., Ohkusa, K., Itoh, Y., Makino, S., & Ezaki, T. (2007). Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters. Japanese Journal of Infectious Diseases, 60(4), 230-4.
Iihara H, et al. Rapid Multiplex Immunofluorescent Assay to Detect Antibodies Against Burkholderia Pseudomallei and Taxonomically Closely Related Nonfermenters. Jpn J Infect Dis. 2007;60(4):230-4. PubMed PMID: 17642542.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid multiplex immunofluorescent assay to detect antibodies against Burkholderia pseudomallei and taxonomically closely related nonfermenters. AU - Iihara,Hirotoshi, AU - Niwa,Takashi, AU - Shah,Mohammad Monir, AU - Nhung,Pham Hong, AU - Song,Sun Xiao, AU - Hayashi,Masahiro, AU - Ohkusa,Kiyofumi, AU - Itoh,Yoshinori, AU - Makino,Sou-ichi, AU - Ezaki,Takayuki, PY - 2007/7/24/pubmed PY - 2007/11/6/medline PY - 2007/7/24/entrez SP - 230 EP - 4 JF - Japanese journal of infectious diseases JO - Jpn J Infect Dis VL - 60 IS - 4 N2 - An indirect immunofluorescent assay to detect antibodies against the lipopolysaccharide (LPS) of Burkholderia pseudomallei and taxonomically closely related species was developed with the Luminex system. LPSs of Pseudomonas aeruginosa, Burkholderia cepacia, Burkholderia thailandensis, Burkholderia vietnamiensis, B. pseudomallei, and Burkholderia mallei were successfully conjugated to Luminex microspheres. Antibodies measured against the LPS of B. pseudomallei-conjugated Luminex beads only cross-reacted with those of two genetically closely related species, B. mallei and B. thailandensis (previously classified as non-pathogenic arabinose-negative B. pseudomallei). However, this system could distinguish other closely related species from B. pseudomallei. This assay is able to detect significantly high levels of anti-LPS antibodies of B. pseudomallei in serum from patients with culture-proven melioidosis. SN - 1344-6304 UR - https://www.unboundmedicine.com/medline/citation/17642542/Rapid_multiplex_immunofluorescent_assay_to_detect_antibodies_against_Burkholderia_pseudomallei_and_taxonomically_closely_related_nonfermenters_ L2 - http://www0.nih.go.jp/JJID/60/230.html DB - PRIME DP - Unbound Medicine ER -