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The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus.
Nat Cell Biol. 2007 Aug; 9(8):923-31.NC

Abstract

Homologous recombination (HR) is crucial for maintaining genome integrity by repairing DNA double-strand breaks (DSBs) and rescuing collapsed replication forks. In contrast, uncontrolled HR can lead to chromosome translocations, loss of heterozygosity, and deletion of repetitive sequences. Controlled HR is particularly important for the preservation of repetitive sequences of the ribosomal gene (rDNA) cluster. Here we show that recombinational repair of a DSB in rDNA in Saccharomyces cerevisiae involves the transient relocalization of the lesion to associate with the recombination machinery at an extranucleolar site. The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related modifier) modification. Remarkably, mutations that abrogate these activities result in the formation of Rad52 foci within the nucleolus and cause rDNA hyperrecombination and the excision of extrachromosomal rDNA circles. Our study also suggests a key role of sumoylation for nucleolar dynamics, perhaps in the compartmentalization of nuclear activities.

Authors+Show Affiliations

Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17643116

Citation

Torres-Rosell, Jordi, et al. "The Smc5-Smc6 Complex and SUMO Modification of Rad52 Regulates Recombinational Repair at the Ribosomal Gene Locus." Nature Cell Biology, vol. 9, no. 8, 2007, pp. 923-31.
Torres-Rosell J, Sunjevaric I, De Piccoli G, et al. The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. Nat Cell Biol. 2007;9(8):923-31.
Torres-Rosell, J., Sunjevaric, I., De Piccoli, G., Sacher, M., Eckert-Boulet, N., Reid, R., Jentsch, S., Rothstein, R., Aragón, L., & Lisby, M. (2007). The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. Nature Cell Biology, 9(8), 923-31.
Torres-Rosell J, et al. The Smc5-Smc6 Complex and SUMO Modification of Rad52 Regulates Recombinational Repair at the Ribosomal Gene Locus. Nat Cell Biol. 2007;9(8):923-31. PubMed PMID: 17643116.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The Smc5-Smc6 complex and SUMO modification of Rad52 regulates recombinational repair at the ribosomal gene locus. AU - Torres-Rosell,Jordi, AU - Sunjevaric,Ivana, AU - De Piccoli,Giacomo, AU - Sacher,Meik, AU - Eckert-Boulet,Nadine, AU - Reid,Robert, AU - Jentsch,Stefan, AU - Rothstein,Rodney, AU - Aragón,Luis, AU - Lisby,Michael, Y1 - 2007/07/22/ PY - 2007/05/11/received PY - 2007/06/28/accepted PY - 2007/7/24/pubmed PY - 2007/10/3/medline PY - 2007/7/24/entrez SP - 923 EP - 31 JF - Nature cell biology JO - Nat Cell Biol VL - 9 IS - 8 N2 - Homologous recombination (HR) is crucial for maintaining genome integrity by repairing DNA double-strand breaks (DSBs) and rescuing collapsed replication forks. In contrast, uncontrolled HR can lead to chromosome translocations, loss of heterozygosity, and deletion of repetitive sequences. Controlled HR is particularly important for the preservation of repetitive sequences of the ribosomal gene (rDNA) cluster. Here we show that recombinational repair of a DSB in rDNA in Saccharomyces cerevisiae involves the transient relocalization of the lesion to associate with the recombination machinery at an extranucleolar site. The nucleolar exclusion of Rad52 recombination foci entails Mre11 and Smc5-Smc6 complexes and depends on Rad52 SUMO (small ubiquitin-related modifier) modification. Remarkably, mutations that abrogate these activities result in the formation of Rad52 foci within the nucleolus and cause rDNA hyperrecombination and the excision of extrachromosomal rDNA circles. Our study also suggests a key role of sumoylation for nucleolar dynamics, perhaps in the compartmentalization of nuclear activities. SN - 1465-7392 UR - https://www.unboundmedicine.com/medline/citation/17643116/The_Smc5_Smc6_complex_and_SUMO_modification_of_Rad52_regulates_recombinational_repair_at_the_ribosomal_gene_locus_ L2 - https://doi.org/10.1038/ncb1619 DB - PRIME DP - Unbound Medicine ER -