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The chlorophenoxy herbicide dicamba and its commercial formulation banvel induce genotoxicity and cytotoxicity in Chinese hamster ovary (CHO) cells.
Mutat Res. 2007 Dec 01; 634(1-2):60-8.MR

Abstract

The sister chromatid exchange (SCE) frequency, the cell-cycle progression analysis, and the single cell gel electrophoresis technique (SCGE, comet assay) were employed as genetic end-points to investigate the geno- and citotoxicity exerted by dicamba and one of its commercial formulation banvel (dicamba 57.71%) on Chinese hamster ovary (CHO) cells. Log-phase cells were treated with 1.0-500.0 microg/ml of the herbicides and harvested 24 h later for SCE and cell-cycle progression analyses. All concentrations assessed of both test compounds induced higher SCE frequencies over control values. SCEs increased in a non-dose-dependent manner neither for the pure compound (r=0.48; P>0.05) nor for the commercial formulation (r=0.58, P>0.05). For the 200.0 microg/ml and 500.0 microg/ml dicamba doses and the 500.0 microg/ml banvel dose, a significant delay in the cell-cycle progression was found. A regression test showed that the proliferation rate index decreased as a function of either the concentration of dicamba (r=-0.98, P<0.05) or banvel (r=-0.88, P<0.01) titrated into cultures in the 1.0-500.0 microg/ml dose-range. SCGE performed on CHO cells after a 90 min pulse-treatment of dicamba and banvel within a 50.0-500.0 microg/ml dose-range revealed a clear increase in dicamba-induced DNA damage as an enhancement of the proportion of slightly damaged and damaged cells for all concentrations used (P<0.01); concomitantly, a decrease of undamaged cells was found over control values (P<0.01). In banvel-treated cells, a similar overall result was registered. Dicamba induced a significant increase both in comet length and width over control values (P<0.01) regardless of its concentration whereas banvel induced the same effect only within 100.0-500.0 microg/ml dose range (P<0.01). As detected by three highly sensitive bioassays, the present results clearly showed the capability of dicamba and banvel to induce DNA and cellular damage on CHO cells.

Authors+Show Affiliations

Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17643342

Citation

González, Norma V., et al. "The Chlorophenoxy Herbicide Dicamba and Its Commercial Formulation Banvel Induce Genotoxicity and Cytotoxicity in Chinese Hamster Ovary (CHO) Cells." Mutation Research, vol. 634, no. 1-2, 2007, pp. 60-8.
González NV, Soloneski S, Larramendy ML. The chlorophenoxy herbicide dicamba and its commercial formulation banvel induce genotoxicity and cytotoxicity in Chinese hamster ovary (CHO) cells. Mutat Res. 2007;634(1-2):60-8.
González, N. V., Soloneski, S., & Larramendy, M. L. (2007). The chlorophenoxy herbicide dicamba and its commercial formulation banvel induce genotoxicity and cytotoxicity in Chinese hamster ovary (CHO) cells. Mutation Research, 634(1-2), 60-8.
González NV, Soloneski S, Larramendy ML. The Chlorophenoxy Herbicide Dicamba and Its Commercial Formulation Banvel Induce Genotoxicity and Cytotoxicity in Chinese Hamster Ovary (CHO) Cells. Mutat Res. 2007 Dec 1;634(1-2):60-8. PubMed PMID: 17643342.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The chlorophenoxy herbicide dicamba and its commercial formulation banvel induce genotoxicity and cytotoxicity in Chinese hamster ovary (CHO) cells. AU - González,Norma V, AU - Soloneski,Sonia, AU - Larramendy,Marcelo L, Y1 - 2007/06/17/ PY - 2006/12/21/received PY - 2007/06/05/revised PY - 2007/06/08/accepted PY - 2007/7/24/pubmed PY - 2008/1/23/medline PY - 2007/7/24/entrez SP - 60 EP - 8 JF - Mutation research JO - Mutat Res VL - 634 IS - 1-2 N2 - The sister chromatid exchange (SCE) frequency, the cell-cycle progression analysis, and the single cell gel electrophoresis technique (SCGE, comet assay) were employed as genetic end-points to investigate the geno- and citotoxicity exerted by dicamba and one of its commercial formulation banvel (dicamba 57.71%) on Chinese hamster ovary (CHO) cells. Log-phase cells were treated with 1.0-500.0 microg/ml of the herbicides and harvested 24 h later for SCE and cell-cycle progression analyses. All concentrations assessed of both test compounds induced higher SCE frequencies over control values. SCEs increased in a non-dose-dependent manner neither for the pure compound (r=0.48; P>0.05) nor for the commercial formulation (r=0.58, P>0.05). For the 200.0 microg/ml and 500.0 microg/ml dicamba doses and the 500.0 microg/ml banvel dose, a significant delay in the cell-cycle progression was found. A regression test showed that the proliferation rate index decreased as a function of either the concentration of dicamba (r=-0.98, P<0.05) or banvel (r=-0.88, P<0.01) titrated into cultures in the 1.0-500.0 microg/ml dose-range. SCGE performed on CHO cells after a 90 min pulse-treatment of dicamba and banvel within a 50.0-500.0 microg/ml dose-range revealed a clear increase in dicamba-induced DNA damage as an enhancement of the proportion of slightly damaged and damaged cells for all concentrations used (P<0.01); concomitantly, a decrease of undamaged cells was found over control values (P<0.01). In banvel-treated cells, a similar overall result was registered. Dicamba induced a significant increase both in comet length and width over control values (P<0.01) regardless of its concentration whereas banvel induced the same effect only within 100.0-500.0 microg/ml dose range (P<0.01). As detected by three highly sensitive bioassays, the present results clearly showed the capability of dicamba and banvel to induce DNA and cellular damage on CHO cells. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/17643342/The_chlorophenoxy_herbicide_dicamba_and_its_commercial_formulation_banvel_induce_genotoxicity_and_cytotoxicity_in_Chinese_hamster_ovary__CHO__cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(07)00192-1 DB - PRIME DP - Unbound Medicine ER -