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Effects of combination of melatonin and dexamethasone on secondary injury in an experimental mice model of spinal cord trauma.
J Pineal Res. 2007 Sep; 43(2):140-53.JP

Abstract

This study investigates the effects of combination therapy with melatonin and dexamethasone on the degree of spinal cord injury caused by the application of vascular clip in mice. Spinal cord injury in mice resulted in severe trauma, characterized by edema, neutrophil infiltration, and apoptosis (measured by terminal deoxynucleotidyltransferase-mediated UTP end labeling staining, and immunoreaction of Bax, Bcl-2, and Fas Ligand). Infiltration of the spinal cord tissue with neutrophils (measured as increase in myeloperoxidase activity) was associated with enhanced immuno- histochemical and functional alterations revealed, respectively, by an increased of tumor necrosis factor (TNF)-alpha immunoreactivity, NOS as well as nitrotyrosine and loss of hind leg movement in spinal cord injury (SCI)-operated mice. In contrast, the degree of neutrophil infiltration at different time points, cytokine expression, histologic damage iNOS expression, apoptosis, was markedly reduced in the tissues obtained from SCI-treated mice with the combination therapy, and the motor recovery was also ameliorated. No anti-inflammatory effect was observed in animals treated with melatonin (10 mg/kg) or with dexamethasone (0.025 mg/kg) alone. This study shows that the combination therapy with melatonin and dexamethasone reduces the degree of secondary damage associated with spinal cord injury in mice, and supports the possible use of melatonin in combination with steroids to reduce the dose and the side effects related with the use of steroids for the management of inflammatory disease.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17645692

Citation

Genovese, Tiziana, et al. "Effects of Combination of Melatonin and Dexamethasone On Secondary Injury in an Experimental Mice Model of Spinal Cord Trauma." Journal of Pineal Research, vol. 43, no. 2, 2007, pp. 140-53.
Genovese T, Mazzon E, Crisafulli C, et al. Effects of combination of melatonin and dexamethasone on secondary injury in an experimental mice model of spinal cord trauma. J Pineal Res. 2007;43(2):140-53.
Genovese, T., Mazzon, E., Crisafulli, C., Esposito, E., Di Paola, R., Muià, C., Di Bella, P., Bramanti, P., & Cuzzocrea, S. (2007). Effects of combination of melatonin and dexamethasone on secondary injury in an experimental mice model of spinal cord trauma. Journal of Pineal Research, 43(2), 140-53.
Genovese T, et al. Effects of Combination of Melatonin and Dexamethasone On Secondary Injury in an Experimental Mice Model of Spinal Cord Trauma. J Pineal Res. 2007;43(2):140-53. PubMed PMID: 17645692.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of combination of melatonin and dexamethasone on secondary injury in an experimental mice model of spinal cord trauma. AU - Genovese,Tiziana, AU - Mazzon,Emanuela, AU - Crisafulli,Concetta, AU - Esposito,Emanuela, AU - Di Paola,Rosanna, AU - Muià,Carmelo, AU - Di Bella,Paolo, AU - Bramanti,Placido, AU - Cuzzocrea,Salvatore, PY - 2007/7/25/pubmed PY - 2007/9/8/medline PY - 2007/7/25/entrez SP - 140 EP - 53 JF - Journal of pineal research JO - J Pineal Res VL - 43 IS - 2 N2 - This study investigates the effects of combination therapy with melatonin and dexamethasone on the degree of spinal cord injury caused by the application of vascular clip in mice. Spinal cord injury in mice resulted in severe trauma, characterized by edema, neutrophil infiltration, and apoptosis (measured by terminal deoxynucleotidyltransferase-mediated UTP end labeling staining, and immunoreaction of Bax, Bcl-2, and Fas Ligand). Infiltration of the spinal cord tissue with neutrophils (measured as increase in myeloperoxidase activity) was associated with enhanced immuno- histochemical and functional alterations revealed, respectively, by an increased of tumor necrosis factor (TNF)-alpha immunoreactivity, NOS as well as nitrotyrosine and loss of hind leg movement in spinal cord injury (SCI)-operated mice. In contrast, the degree of neutrophil infiltration at different time points, cytokine expression, histologic damage iNOS expression, apoptosis, was markedly reduced in the tissues obtained from SCI-treated mice with the combination therapy, and the motor recovery was also ameliorated. No anti-inflammatory effect was observed in animals treated with melatonin (10 mg/kg) or with dexamethasone (0.025 mg/kg) alone. This study shows that the combination therapy with melatonin and dexamethasone reduces the degree of secondary damage associated with spinal cord injury in mice, and supports the possible use of melatonin in combination with steroids to reduce the dose and the side effects related with the use of steroids for the management of inflammatory disease. SN - 0742-3098 UR - https://www.unboundmedicine.com/medline/citation/17645692/Effects_of_combination_of_melatonin_and_dexamethasone_on_secondary_injury_in_an_experimental_mice_model_of_spinal_cord_trauma_ L2 - https://doi.org/10.1111/j.1600-079X.2007.00454.x DB - PRIME DP - Unbound Medicine ER -