Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses.
Naturwissenschaften. 2008 Jan; 95(1):25-32.N

Abstract

Several immunosuppressive factors are associated with parasitism of an endoparasitoid wasp, Cotesia plutellae, on the diamondback moth, Plutella xylostella. C. plutellae bracovirus (CpBV) encodes a large number of putative protein tyrosine phosphatases (PTPs), which may play a role in inhibiting host cellular immunity. To address this inhibitory hypothesis of CpBV-PTPs, we performed transient expression of individual CpBV-PTPs in hemocytes of the beet armyworm, Spodoptera exigua, and analyzed their cellular immune responses. Two different forms of CpBV-PTPs were chosen and cloned into a eukaryotic expression vector under the control of the p10 promoter of baculovirus: one with the normal cysteine active site (CpBV-PTP1) and the other with a mutated active site (CpBV-PTP5). The hemocytes transfected with CpBV-PTP1 significantly increased in PTP activity compared to control hemocytes, but those with CpBV-PTP5 exhibited a significant decrease in the PTP activity. All transfected hemocytes exhibited a significant reduction in both cell spreading and encapsulation activities compared to control hemocytes. Co-transfection of CpBV-PTP1 together with its double-stranded RNA reduced the messenger RNA (mRNA) level of CpBV-PTP1 and resulted in recovery of both hemocyte behaviors. This is the first report demonstrating that the polydnaviral PTPs can manipulate PTP activity of the hemocytes to interrupt cellular immune responses.

Authors+Show Affiliations

Ibrahim AM

Department of Bioresource Sciences, Andong National University, Andong, 760-749, South Korea.

Kim Y

No affiliation info available

MeSH

AnimalsCodon, TerminatorDNA PrimersDNA, ViralHemocytesImmunity, CellularImmunosuppressionPolydnaviridaeProtein Tyrosine PhosphatasesRestriction MappingReverse Transcriptase Polymerase Chain ReactionSpodopteraTransfectionViral ProteinsWasps

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17646950

Citation

Ibrahim, Ahmed M A., and Yonggyun Kim. "Transient Expression of Protein Tyrosine Phosphatases Encoded in Cotesia Plutellae Bracovirus Inhibits Insect Cellular Immune Responses." Die Naturwissenschaften, vol. 95, no. 1, 2008, pp. 25-32.

Ibrahim AM, Kim Y. Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses. Naturwissenschaften. 2008;95(1):25-32.

Ibrahim, A. M., & Kim, Y. (2008). Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses. Die Naturwissenschaften, 95(1), 25-32.

Ibrahim AM, Kim Y. Transient Expression of Protein Tyrosine Phosphatases Encoded in Cotesia Plutellae Bracovirus Inhibits Insect Cellular Immune Responses. Naturwissenschaften. 2008;95(1):25-32. PubMed PMID: 17646950.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses. AU - Ibrahim,Ahmed M A, AU - Kim,Yonggyun, Y1 - 2007/07/24/ PY - 2006/10/23/received PY - 2007/06/28/accepted PY - 2007/05/21/revised PY - 2007/7/25/pubmed PY - 2009/5/21/medline PY - 2007/7/25/entrez SP - 25 EP - 32 JF - Die Naturwissenschaften JO - Naturwissenschaften VL - 95 IS - 1 N2 - Several immunosuppressive factors are associated with parasitism of an endoparasitoid wasp, Cotesia plutellae, on the diamondback moth, Plutella xylostella. C. plutellae bracovirus (CpBV) encodes a large number of putative protein tyrosine phosphatases (PTPs), which may play a role in inhibiting host cellular immunity. To address this inhibitory hypothesis of CpBV-PTPs, we performed transient expression of individual CpBV-PTPs in hemocytes of the beet armyworm, Spodoptera exigua, and analyzed their cellular immune responses. Two different forms of CpBV-PTPs were chosen and cloned into a eukaryotic expression vector under the control of the p10 promoter of baculovirus: one with the normal cysteine active site (CpBV-PTP1) and the other with a mutated active site (CpBV-PTP5). The hemocytes transfected with CpBV-PTP1 significantly increased in PTP activity compared to control hemocytes, but those with CpBV-PTP5 exhibited a significant decrease in the PTP activity. All transfected hemocytes exhibited a significant reduction in both cell spreading and encapsulation activities compared to control hemocytes. Co-transfection of CpBV-PTP1 together with its double-stranded RNA reduced the messenger RNA (mRNA) level of CpBV-PTP1 and resulted in recovery of both hemocyte behaviors. This is the first report demonstrating that the polydnaviral PTPs can manipulate PTP activity of the hemocytes to interrupt cellular immune responses. SN - 1432-1904 UR - https://www.unboundmedicine.com/medline/citation/17646950/Transient_expression_of_protein_tyrosine_phosphatases_encoded_in_Cotesia_plutellae_bracovirus_inhibits_insect_cellular_immune_responses_ L2 - https://dx.doi.org/10.1007/s00114-007-0290-7 DB - PRIME DP - Unbound Medicine ER -

Tags

Transient expression of protein tyrosine phosphatases encoded in Cotesia plutellae bracovirus inhibits insect cellular immune responses.Naturwissenschaften. 2008 Jan; 95(1):25-32.N