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[Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig].
Zhonghua Yi Xue Za Zhi. 2007 Apr 10; 87(14):995-8.ZY

Abstract

OBJECTIVE

To investigate the effects of dry powder of ShenSongYangXin capsule on sodium current (I(Na)) and L-type calcium current (I(Ca, L)) in ventricular myocytes.

METHODS

Ventricular myocytes were isolated from guinea pig. Solution of the dry powder of ShenSongYangXin capsule of the concentrations of 1%, 0.5%, and 0.25% was added into the suspension of the myocytes. Whole cell patch-clamp technique was used to detect the I(Na) and I(Ca, L) in the ventricular myocytes.

RESULT

ShenSongYangXin decreased the peak I(Na) from 27.2 +/- 5.4 (pA/pF) to 14.9 +/- 2.8 (pA/pF), with a decrease rate of 44.8% +/- 7.7% (n = 5, P < 0.05). The solution of dry powder of ShenSongYangXin of the concentrations of 1%, 0.5%, and 0.25% decreased the peak L-type calcium channel current (I(ca, L)) significantly in a concentration dependent manner with the reduction rates of 50.7% +/- 5.6%, 44.8% +/- 6.5%, and 19.2% +/- 1.1% respectively (n = 5, all P < 0.05). ShenSongYangXin shifted up both the I approximately 1V curve of I(Na) and that of I(Ca, L), while their active, peak and reverse potentials didn't change.

CONCLUSION

ShenSongYangXin blocks both I(Na) and I(Ca, L), which may contributes to some of its antiarrhythmic effect.

Links

Publisher Full Text

Authors+Show Affiliations

Li N
Center for Arrhythmia Diagnosis and Treatment, Fuwai Cardiovascular Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100037, China.
Huo YP
No affiliation info available
Ma KJ
No affiliation info available
Sun Q
No affiliation info available
Pu JL
No affiliation info available

MeSH

AnimalsCalcium Channels, L-TypeCapsulesDose-Response Relationship, DrugDrug CombinationsDrugs, Chinese HerbalGuinea PigsHeart VentriclesMembrane PotentialsMyocytes, CardiacPatch-Clamp TechniquesPowders

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17650428

Citation

Li, Ning, et al. "[Effects of Solution of Dry Powder of ShenSongYangXin Capsule On Sodium Current and L-type Calcium Current in Ventricular Myocytes: Experiment With Guinea Pig]." Zhonghua Yi Xue Za Zhi, vol. 87, no. 14, 2007, pp. 995-8.
Li N, Huo YP, Ma KJ, et al. [Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig]. Zhonghua Yi Xue Za Zhi. 2007;87(14):995-8.
Li, N., Huo, Y. P., Ma, K. J., Sun, Q., & Pu, J. L. (2007). [Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig]. Zhonghua Yi Xue Za Zhi, 87(14), 995-8.
Li N, et al. [Effects of Solution of Dry Powder of ShenSongYangXin Capsule On Sodium Current and L-type Calcium Current in Ventricular Myocytes: Experiment With Guinea Pig]. Zhonghua Yi Xue Za Zhi. 2007 Apr 10;87(14):995-8. PubMed PMID: 17650428.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of solution of dry powder of ShenSongYangXin capsule on sodium current and L-type calcium current in ventricular myocytes: experiment with guinea pig]. AU - Li,Ning, AU - Huo,You-ping, AU - Ma,Ke-juan, AU - Sun,Qi, AU - Pu,Jie-lin, PY - 2007/7/26/pubmed PY - 2008/8/30/medline PY - 2007/7/26/entrez SP - 995 EP - 8 JF - Zhonghua yi xue za zhi JO - Zhonghua Yi Xue Za Zhi VL - 87 IS - 14 N2 - OBJECTIVE: To investigate the effects of dry powder of ShenSongYangXin capsule on sodium current (I(Na)) and L-type calcium current (I(Ca, L)) in ventricular myocytes. METHODS: Ventricular myocytes were isolated from guinea pig. Solution of the dry powder of ShenSongYangXin capsule of the concentrations of 1%, 0.5%, and 0.25% was added into the suspension of the myocytes. Whole cell patch-clamp technique was used to detect the I(Na) and I(Ca, L) in the ventricular myocytes. RESULT: ShenSongYangXin decreased the peak I(Na) from 27.2 +/- 5.4 (pA/pF) to 14.9 +/- 2.8 (pA/pF), with a decrease rate of 44.8% +/- 7.7% (n = 5, P < 0.05). The solution of dry powder of ShenSongYangXin of the concentrations of 1%, 0.5%, and 0.25% decreased the peak L-type calcium channel current (I(ca, L)) significantly in a concentration dependent manner with the reduction rates of 50.7% +/- 5.6%, 44.8% +/- 6.5%, and 19.2% +/- 1.1% respectively (n = 5, all P < 0.05). ShenSongYangXin shifted up both the I approximately 1V curve of I(Na) and that of I(Ca, L), while their active, peak and reverse potentials didn't change. CONCLUSION: ShenSongYangXin blocks both I(Na) and I(Ca, L), which may contributes to some of its antiarrhythmic effect. SN - 0376-2491 UR - https://www.unboundmedicine.com/medline/citation/17650428/[Effects_of_solution_of_dry_powder_of_ShenSongYangXin_capsule_on_sodium_current_and_L_type_calcium_current_in_ventricular_myocytes:_experiment_with_guinea_pig]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=0376-2491&amp;year=2007&amp;vol=87&amp;issue=14&amp;fpage=995 DB - PRIME DP - Unbound Medicine ER -