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Sequence analysis and molecular characterization of larval midgut cDNA transcripts encoding peptidases from the yellow mealworm, Tenebrio molitor L.
Insect Mol Biol. 2007 Aug; 16(4):455-68.IM

Abstract

Peptidase sequences were analysed in randomly picked clones from cDNA libraries of the anterior or posterior midgut or whole larvae of the yellow mealworm, Tenebrio molitor Linnaeus. Of a total of 1528 sequences, 92 encoded potential peptidases, from which 50 full-length cDNA sequences were obtained, including serine and cysteine proteinases and metallopeptidases. Serine proteinase transcripts were predominant in the posterior midgut, whereas transcripts encoding cysteine and metallopeptidases were mainly found in the anterior midgut. Alignments with other proteinases indicated that 40% of the serine proteinase sequences were serine proteinase homologues, and the remaining ones were identified as either trypsin, chymotrypsin or other serine proteinases. Cysteine proteinase sequences included cathepsin B- and L-like proteinases, and metallopeptidase transcripts were similar to carboxypeptidase A. Northern blot analysis of representative sequences demonstrated the differential expression profile of selected transcripts across five developmental stages of Te. molitor. These sequences provide insights into peptidases in coleopteran insects as a basis to study the response of coleopteran larvae to external stimuli and to evaluate regulatory features of the response.

Authors+Show Affiliations

Department of Entomology, Kansas State University, Manhattan, KS, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

17651235

Citation

Prabhakar, S, et al. "Sequence Analysis and Molecular Characterization of Larval Midgut cDNA Transcripts Encoding Peptidases From the Yellow Mealworm, Tenebrio Molitor L." Insect Molecular Biology, vol. 16, no. 4, 2007, pp. 455-68.
Prabhakar S, Chen MS, Elpidina EN, et al. Sequence analysis and molecular characterization of larval midgut cDNA transcripts encoding peptidases from the yellow mealworm, Tenebrio molitor L. Insect Mol Biol. 2007;16(4):455-68.
Prabhakar, S., Chen, M. S., Elpidina, E. N., Vinokurov, K. S., Smith, C. M., Marshall, J., & Oppert, B. (2007). Sequence analysis and molecular characterization of larval midgut cDNA transcripts encoding peptidases from the yellow mealworm, Tenebrio molitor L. Insect Molecular Biology, 16(4), 455-68.
Prabhakar S, et al. Sequence Analysis and Molecular Characterization of Larval Midgut cDNA Transcripts Encoding Peptidases From the Yellow Mealworm, Tenebrio Molitor L. Insect Mol Biol. 2007;16(4):455-68. PubMed PMID: 17651235.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Sequence analysis and molecular characterization of larval midgut cDNA transcripts encoding peptidases from the yellow mealworm, Tenebrio molitor L. AU - Prabhakar,S, AU - Chen,M-S, AU - Elpidina,E N, AU - Vinokurov,K S, AU - Smith,C M, AU - Marshall,J, AU - Oppert,B, PY - 2007/7/27/pubmed PY - 2007/9/20/medline PY - 2007/7/27/entrez SP - 455 EP - 68 JF - Insect molecular biology JO - Insect Mol Biol VL - 16 IS - 4 N2 - Peptidase sequences were analysed in randomly picked clones from cDNA libraries of the anterior or posterior midgut or whole larvae of the yellow mealworm, Tenebrio molitor Linnaeus. Of a total of 1528 sequences, 92 encoded potential peptidases, from which 50 full-length cDNA sequences were obtained, including serine and cysteine proteinases and metallopeptidases. Serine proteinase transcripts were predominant in the posterior midgut, whereas transcripts encoding cysteine and metallopeptidases were mainly found in the anterior midgut. Alignments with other proteinases indicated that 40% of the serine proteinase sequences were serine proteinase homologues, and the remaining ones were identified as either trypsin, chymotrypsin or other serine proteinases. Cysteine proteinase sequences included cathepsin B- and L-like proteinases, and metallopeptidase transcripts were similar to carboxypeptidase A. Northern blot analysis of representative sequences demonstrated the differential expression profile of selected transcripts across five developmental stages of Te. molitor. These sequences provide insights into peptidases in coleopteran insects as a basis to study the response of coleopteran larvae to external stimuli and to evaluate regulatory features of the response. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/17651235/Sequence_analysis_and_molecular_characterization_of_larval_midgut_cDNA_transcripts_encoding_peptidases_from_the_yellow_mealworm_Tenebrio_molitor_L_ L2 - https://doi.org/10.1111/j.1365-2583.2007.00740.x DB - PRIME DP - Unbound Medicine ER -