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Gene organization of a novel defensin of Ixodes ricinus: first annotation of an intron/exon structure in a hard tick defensin gene and first evidence of the occurrence of two isoforms of one member of the arthropod defensin family.
Insect Mol Biol. 2007 Aug; 16(4):501-7.IM

Abstract

Antimicrobial peptides (defensins) are effectors of the immune system. Herein, we describe a novel Ixodes ricinus defensin gene(s), analyse its structure and compare it with other known antimicrobial peptides from different tick species. For the first time, an intron/exon structure is discovered in a hard-tick defensin gene. The intron/exon genomic organization of the gene is similar to the organization in Ornithodoros moubata, but not to that of the intronless defensins of Dermacentor variabilis and Ixodes scapularis. The analysis of the deduced amino acid sequences of different recombinants from the I. ricinus cDNA library reveals the presence of two defensin isoforms with three amino acid substitutions. Whether or not these substitutions affect the biological properties of the peptides is currently unknown. The expression of the defensin gene is strongly induced in the tick midgut after infection with Borrelia burgdorferi.

Authors+Show Affiliations

Faculty of Biological Sciences, University of South Bohemia and Biology Centre, Institute of Parasitology AS CR, Ceské Budejovice, Czech Republic. natasha@paru.cas.czNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17651239

Citation

Rudenko, N, et al. "Gene Organization of a Novel Defensin of Ixodes Ricinus: First Annotation of an Intron/exon Structure in a Hard Tick Defensin Gene and First Evidence of the Occurrence of Two Isoforms of One Member of the Arthropod Defensin Family." Insect Molecular Biology, vol. 16, no. 4, 2007, pp. 501-7.
Rudenko N, Golovchenko M, Grubhoffer L. Gene organization of a novel defensin of Ixodes ricinus: first annotation of an intron/exon structure in a hard tick defensin gene and first evidence of the occurrence of two isoforms of one member of the arthropod defensin family. Insect Mol Biol. 2007;16(4):501-7.
Rudenko, N., Golovchenko, M., & Grubhoffer, L. (2007). Gene organization of a novel defensin of Ixodes ricinus: first annotation of an intron/exon structure in a hard tick defensin gene and first evidence of the occurrence of two isoforms of one member of the arthropod defensin family. Insect Molecular Biology, 16(4), 501-7.
Rudenko N, Golovchenko M, Grubhoffer L. Gene Organization of a Novel Defensin of Ixodes Ricinus: First Annotation of an Intron/exon Structure in a Hard Tick Defensin Gene and First Evidence of the Occurrence of Two Isoforms of One Member of the Arthropod Defensin Family. Insect Mol Biol. 2007;16(4):501-7. PubMed PMID: 17651239.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene organization of a novel defensin of Ixodes ricinus: first annotation of an intron/exon structure in a hard tick defensin gene and first evidence of the occurrence of two isoforms of one member of the arthropod defensin family. AU - Rudenko,N, AU - Golovchenko,M, AU - Grubhoffer,L, PY - 2007/7/27/pubmed PY - 2007/9/20/medline PY - 2007/7/27/entrez SP - 501 EP - 7 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 16 IS - 4 N2 - Antimicrobial peptides (defensins) are effectors of the immune system. Herein, we describe a novel Ixodes ricinus defensin gene(s), analyse its structure and compare it with other known antimicrobial peptides from different tick species. For the first time, an intron/exon structure is discovered in a hard-tick defensin gene. The intron/exon genomic organization of the gene is similar to the organization in Ornithodoros moubata, but not to that of the intronless defensins of Dermacentor variabilis and Ixodes scapularis. The analysis of the deduced amino acid sequences of different recombinants from the I. ricinus cDNA library reveals the presence of two defensin isoforms with three amino acid substitutions. Whether or not these substitutions affect the biological properties of the peptides is currently unknown. The expression of the defensin gene is strongly induced in the tick midgut after infection with Borrelia burgdorferi. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/17651239/Gene_organization_of_a_novel_defensin_of_Ixodes_ricinus:_first_annotation_of_an_intron/exon_structure_in_a_hard_tick_defensin_gene_and_first_evidence_of_the_occurrence_of_two_isoforms_of_one_member_of_the_arthropod_defensin_family_ L2 - https://doi.org/10.1111/j.1365-2583.2007.00745.x DB - PRIME DP - Unbound Medicine ER -