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[Effect of amiloride on the pathology of a rat model of chronic obstructive pulmonary disease].
Zhonghua Jie He He Hu Xi Za Zhi. 2007 May; 30(5):363-7.ZJ

Abstract

OBJECTIVE

To study the effects of amiloride, an inhibitor of urokinase, on pathological and pathophysiological changes of chronic obstructive pulmonary disease (COPD) model, and to investigate the role of urokinase plasminogen activator system components in the pathogenesis of COPD.

METHODS

Healthy Wistar rats (n = 24) were randomly divided into a control group, a model group and an amiloride group. After 7 weeks, lung function measurements were performed. The total and different white blood cell counts of bronchoalveolar lavage fluid (BALF) were determined, and collagen deposition of lung sections was observed by picrosirius staining. The expressions of u-PA, u-PAR and PAI-1 in bronchial lung tissues were examined by immunohistochemical analysis.

RESULTS

In the model group the expiratory resistance (Re) was significantly higher than that of the control group and the amiloride group, while forced expiratory volume in the 0.3 s/forced expiratory capacity (FEV(0.3)/FVC%) and peak expiratory flow (PEF) were significantly lower than those of the other two groups. Significant increase in total white blood cells and percentage of neutrophils and macrophages in BALF was found in the model group as compared to the control group and the amiloride group. The collagen deposition, mainly type I collagen, in airway walls was significantly increased in the model group. The levels of u-PA, u-PAR and PAI-1 in the model group [(0.166 +/- 0.010), (0.158 +/- 0.024), (0.171 +/- 0.012), respectively] were significantly higher than those in the control group [(0.137 +/- 0.015), (0.122 +/- 0.009), (0.144 +/- 0.005), respectively] and the amiloride group [(0.126 +/- 0.004), (0.120 +/- 0.010), (0.122 +/- 0.004), respectively]. F values were 32.463, 4.094, 79.562 respectively, and all P < 0.001. Moreover, u-PAR protein level was positively correlated with neutrophils in BALF in the model group (r = 0.754, P = 0.031).

CONCLUSION

Administration of u-PA inhibitor-amiloride significantly alleviated airway inflammation and the pathological changes in COPD rats, suggesting that the u-PA system components are key mediators regulating the inflammatory reaction and tissue remodeling in the pathological process of COPD.

Authors+Show Affiliations

Department of Respiratory Medicine, Qilu Hospital of Shandong University, Jinan 250012, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17651644

Citation

Han, Xiu-di, et al. "[Effect of Amiloride On the Pathology of a Rat Model of Chronic Obstructive Pulmonary Disease]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 30, no. 5, 2007, pp. 363-7.
Han XD, Xiao W, Liu CX, et al. [Effect of amiloride on the pathology of a rat model of chronic obstructive pulmonary disease]. Zhonghua Jie He He Hu Xi Za Zhi. 2007;30(5):363-7.
Han, X. D., Xiao, W., Liu, C. X., Xu, X., Wang, H. C., & Xing, Y. (2007). [Effect of amiloride on the pathology of a rat model of chronic obstructive pulmonary disease]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 30(5), 363-7.
Han XD, et al. [Effect of Amiloride On the Pathology of a Rat Model of Chronic Obstructive Pulmonary Disease]. Zhonghua Jie He He Hu Xi Za Zhi. 2007;30(5):363-7. PubMed PMID: 17651644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effect of amiloride on the pathology of a rat model of chronic obstructive pulmonary disease]. AU - Han,Xiu-di, AU - Xiao,Wei, AU - Liu,Chun-xi, AU - Xu,Xia, AU - Wang,Hong-chao, AU - Xing,Ying, PY - 2007/7/27/pubmed PY - 2010/8/18/medline PY - 2007/7/27/entrez SP - 363 EP - 7 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 30 IS - 5 N2 - OBJECTIVE: To study the effects of amiloride, an inhibitor of urokinase, on pathological and pathophysiological changes of chronic obstructive pulmonary disease (COPD) model, and to investigate the role of urokinase plasminogen activator system components in the pathogenesis of COPD. METHODS: Healthy Wistar rats (n = 24) were randomly divided into a control group, a model group and an amiloride group. After 7 weeks, lung function measurements were performed. The total and different white blood cell counts of bronchoalveolar lavage fluid (BALF) were determined, and collagen deposition of lung sections was observed by picrosirius staining. The expressions of u-PA, u-PAR and PAI-1 in bronchial lung tissues were examined by immunohistochemical analysis. RESULTS: In the model group the expiratory resistance (Re) was significantly higher than that of the control group and the amiloride group, while forced expiratory volume in the 0.3 s/forced expiratory capacity (FEV(0.3)/FVC%) and peak expiratory flow (PEF) were significantly lower than those of the other two groups. Significant increase in total white blood cells and percentage of neutrophils and macrophages in BALF was found in the model group as compared to the control group and the amiloride group. The collagen deposition, mainly type I collagen, in airway walls was significantly increased in the model group. The levels of u-PA, u-PAR and PAI-1 in the model group [(0.166 +/- 0.010), (0.158 +/- 0.024), (0.171 +/- 0.012), respectively] were significantly higher than those in the control group [(0.137 +/- 0.015), (0.122 +/- 0.009), (0.144 +/- 0.005), respectively] and the amiloride group [(0.126 +/- 0.004), (0.120 +/- 0.010), (0.122 +/- 0.004), respectively]. F values were 32.463, 4.094, 79.562 respectively, and all P < 0.001. Moreover, u-PAR protein level was positively correlated with neutrophils in BALF in the model group (r = 0.754, P = 0.031). CONCLUSION: Administration of u-PA inhibitor-amiloride significantly alleviated airway inflammation and the pathological changes in COPD rats, suggesting that the u-PA system components are key mediators regulating the inflammatory reaction and tissue remodeling in the pathological process of COPD. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/17651644/[Effect_of_amiloride_on_the_pathology_of_a_rat_model_of_chronic_obstructive_pulmonary_disease]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&amp;issn=1001-0939&amp;year=2007&amp;vol=30&amp;issue=5&amp;fpage=363 DB - PRIME DP - Unbound Medicine ER -