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IgA anti-actin antibodies ELISA in coeliac disease: a multicentre study.
Dig Liver Dis 2007; 39(9):818-23DL

Abstract

BACKGROUND

Previous studies have demonstrated that serum anti-actin antibodies are a reliable marker of intestinal damage severity in coeliac disease.

AIMS

To validate in a multicentre study the clinical usefulness of serum IgA anti-actin antibody ELISA and its possible use in monitoring intestinal mucosa lesions during gluten-free diet.

PATIENTS AND METHODS

Four centres recruited 205 newly diagnosed coeliac disease patients with villous atrophy, 80 healthy controls and 81 "disease" controls. Twelve coeliac disease patients on gluten-free diet but with persistent symptoms underwent serum IgA anti-actin antibody assay and intestinal histology evaluation. IgA anti-actin antibody ELISA was performed with a commercial kit. All coeliac disease patients underwent intestinal histology study.

RESULTS

IgA anti-actin antibodies showed a sensitivity of 80% and a specificity of 85% in the diagnosis of coeliac disease patients with villous atrophy. The area under the receiving operator curve for anti-actin antibodies was 0.873 [95% C.I. 0.805-0.899]. Serum anti-actin antibodies values were significantly higher in coeliac disease patients than in healthy or "disease" controls (P<0.0001). Serum anti-actin antibodies were positive in 41 of the 60 coeliac disease patients with mild intestinal histology lesions (69%) and in 123 of the 145 with severe lesions (85.3%) (P<0.05). There was a significant inverse correlation between anti-actin antibody values and the villi/crypts ratio (r=-0.423; P<0.0001). In the 12 coeliac disease patients on gluten-free diet who underwent re-evaluation as they were persistently symptomatic, intestinal histology showed three cases with persistent villous atrophy: all of these were positive for serum anti-actin antibodies ELISA, whereas both serum anti-tTG and EmAs were negative. The other nine patients showed normal intestinal villi and were negative for serum anti-actin antibodies.

CONCLUSIONS

Anti-actin antibodies are a reliable marker of severe intestinal mucosa damage in coeliac disease patients and a simple ELISA technique offers an accurate method for their determination. These antibodies seem to be a very reliable marker of persistent intestinal damage in coeliac disease patients.

Authors+Show Affiliations

Internal Medicine, University Hospital, Palermo, via del Vespro 141, 90127 Palermo, Italy. acarroccio@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't
Validation Studies

Language

eng

PubMed ID

17652043

Citation

Carroccio, A, et al. "IgA Anti-actin Antibodies ELISA in Coeliac Disease: a Multicentre Study." Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, vol. 39, no. 9, 2007, pp. 818-23.
Carroccio A, Brusca I, Iacono G, et al. IgA anti-actin antibodies ELISA in coeliac disease: a multicentre study. Dig Liver Dis. 2007;39(9):818-23.
Carroccio, A., Brusca, I., Iacono, G., Alessio, M. G., Sonzogni, A., Di Prima, L., ... La Chiusa, S. M. (2007). IgA anti-actin antibodies ELISA in coeliac disease: a multicentre study. Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 39(9), pp. 818-23.
Carroccio A, et al. IgA Anti-actin Antibodies ELISA in Coeliac Disease: a Multicentre Study. Dig Liver Dis. 2007;39(9):818-23. PubMed PMID: 17652043.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IgA anti-actin antibodies ELISA in coeliac disease: a multicentre study. AU - Carroccio,A, AU - Brusca,I, AU - Iacono,G, AU - Alessio,M G, AU - Sonzogni,A, AU - Di Prima,L, AU - Barrale,M, AU - Ottomano,C, AU - Ambrosiano,G, AU - Teresi,S, AU - D'Angelo,A, AU - Pirrone,G, AU - Cefalù,B, AU - Scalici,C, AU - La Chiusa,S M, Y1 - 2007/07/25/ PY - 2007/02/20/received PY - 2007/05/25/revised PY - 2007/06/05/accepted PY - 2007/7/27/pubmed PY - 2007/12/6/medline PY - 2007/7/27/entrez SP - 818 EP - 23 JF - Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver JO - Dig Liver Dis VL - 39 IS - 9 N2 - BACKGROUND: Previous studies have demonstrated that serum anti-actin antibodies are a reliable marker of intestinal damage severity in coeliac disease. AIMS: To validate in a multicentre study the clinical usefulness of serum IgA anti-actin antibody ELISA and its possible use in monitoring intestinal mucosa lesions during gluten-free diet. PATIENTS AND METHODS: Four centres recruited 205 newly diagnosed coeliac disease patients with villous atrophy, 80 healthy controls and 81 "disease" controls. Twelve coeliac disease patients on gluten-free diet but with persistent symptoms underwent serum IgA anti-actin antibody assay and intestinal histology evaluation. IgA anti-actin antibody ELISA was performed with a commercial kit. All coeliac disease patients underwent intestinal histology study. RESULTS: IgA anti-actin antibodies showed a sensitivity of 80% and a specificity of 85% in the diagnosis of coeliac disease patients with villous atrophy. The area under the receiving operator curve for anti-actin antibodies was 0.873 [95% C.I. 0.805-0.899]. Serum anti-actin antibodies values were significantly higher in coeliac disease patients than in healthy or "disease" controls (P<0.0001). Serum anti-actin antibodies were positive in 41 of the 60 coeliac disease patients with mild intestinal histology lesions (69%) and in 123 of the 145 with severe lesions (85.3%) (P<0.05). There was a significant inverse correlation between anti-actin antibody values and the villi/crypts ratio (r=-0.423; P<0.0001). In the 12 coeliac disease patients on gluten-free diet who underwent re-evaluation as they were persistently symptomatic, intestinal histology showed three cases with persistent villous atrophy: all of these were positive for serum anti-actin antibodies ELISA, whereas both serum anti-tTG and EmAs were negative. The other nine patients showed normal intestinal villi and were negative for serum anti-actin antibodies. CONCLUSIONS: Anti-actin antibodies are a reliable marker of severe intestinal mucosa damage in coeliac disease patients and a simple ELISA technique offers an accurate method for their determination. These antibodies seem to be a very reliable marker of persistent intestinal damage in coeliac disease patients. SN - 1590-8658 UR - https://www.unboundmedicine.com/medline/citation/17652043/IgA_anti_actin_antibodies_ELISA_in_coeliac_disease:_a_multicentre_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1590-8658(07)00246-0 DB - PRIME DP - Unbound Medicine ER -