Tags

Type your tag names separated by a space and hit enter

Cannabinoid-2 receptor agonist HU-308 protects against hepatic ischemia/reperfusion injury by attenuating oxidative stress, inflammatory response, and apoptosis.
J Leukoc Biol. 2007 Dec; 82(6):1382-9.JL

Abstract

In this study, we have investigated the role of the cannabinoid CB(2) (CB(2)) receptor in an in vivo mouse model of hepatic ischemia/reperfusion (I/R) injury. In addition, we have assessed the role of the CB(2) receptor in TNF-alpha-induced ICAM-1 and VCAM-1 expression in human liver sinusoidal endothelial cells (HLSECs) and in the adhesion of human neutrophils to HLSECs in vitro. The potent CB(2) receptor agonist HU-308, given prior to the induction of I/R, significantly attenuated the extent of liver damage (measured by serum alanine aminotransferase and lactate dehydrogenase) and decreased serum and tissue TNF-alpha, MIP-1alpha, and MIP-2 levels, tissue lipid peroxidation, neutrophil infiltration, DNA fragmentation, and caspase 3 activity. The protective effect of HU-308 against liver damage was also preserved when given right after the ischemic episode. HU-308 also attenuated the TNF-alpha-induced ICAM-1 and VCAM-1 expression in HLSECs, which expressed CB(2) receptors, and the adhesion of human neutrophils to HLSECs in vitro. These findings suggest that selective CB(2) receptor agonists may represent a novel, protective strategy against I/R injury by attenuating oxidative stress, inflammatory response, and apoptosis.

Authors+Show Affiliations

Section on Oxidative Stress Tissue Injury, Laboratory of Physiologic Studies, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland 20892-9413, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Intramural

Language

eng

PubMed ID

17652447

Citation

Rajesh, Mohanraj, et al. "Cannabinoid-2 Receptor Agonist HU-308 Protects Against Hepatic Ischemia/reperfusion Injury By Attenuating Oxidative Stress, Inflammatory Response, and Apoptosis." Journal of Leukocyte Biology, vol. 82, no. 6, 2007, pp. 1382-9.
Rajesh M, Pan H, Mukhopadhyay P, et al. Cannabinoid-2 receptor agonist HU-308 protects against hepatic ischemia/reperfusion injury by attenuating oxidative stress, inflammatory response, and apoptosis. J Leukoc Biol. 2007;82(6):1382-9.
Rajesh, M., Pan, H., Mukhopadhyay, P., Bátkai, S., Osei-Hyiaman, D., Haskó, G., Liaudet, L., Gao, B., & Pacher, P. (2007). Cannabinoid-2 receptor agonist HU-308 protects against hepatic ischemia/reperfusion injury by attenuating oxidative stress, inflammatory response, and apoptosis. Journal of Leukocyte Biology, 82(6), 1382-9.
Rajesh M, et al. Cannabinoid-2 Receptor Agonist HU-308 Protects Against Hepatic Ischemia/reperfusion Injury By Attenuating Oxidative Stress, Inflammatory Response, and Apoptosis. J Leukoc Biol. 2007;82(6):1382-9. PubMed PMID: 17652447.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cannabinoid-2 receptor agonist HU-308 protects against hepatic ischemia/reperfusion injury by attenuating oxidative stress, inflammatory response, and apoptosis. AU - Rajesh,Mohanraj, AU - Pan,Hao, AU - Mukhopadhyay,Partha, AU - Bátkai,Sándor, AU - Osei-Hyiaman,Douglas, AU - Haskó,György, AU - Liaudet,Lucas, AU - Gao,Bin, AU - Pacher,Pál, Y1 - 2007/07/25/ PY - 2007/7/27/pubmed PY - 2008/1/24/medline PY - 2007/7/27/entrez SP - 1382 EP - 9 JF - Journal of leukocyte biology JO - J Leukoc Biol VL - 82 IS - 6 N2 - In this study, we have investigated the role of the cannabinoid CB(2) (CB(2)) receptor in an in vivo mouse model of hepatic ischemia/reperfusion (I/R) injury. In addition, we have assessed the role of the CB(2) receptor in TNF-alpha-induced ICAM-1 and VCAM-1 expression in human liver sinusoidal endothelial cells (HLSECs) and in the adhesion of human neutrophils to HLSECs in vitro. The potent CB(2) receptor agonist HU-308, given prior to the induction of I/R, significantly attenuated the extent of liver damage (measured by serum alanine aminotransferase and lactate dehydrogenase) and decreased serum and tissue TNF-alpha, MIP-1alpha, and MIP-2 levels, tissue lipid peroxidation, neutrophil infiltration, DNA fragmentation, and caspase 3 activity. The protective effect of HU-308 against liver damage was also preserved when given right after the ischemic episode. HU-308 also attenuated the TNF-alpha-induced ICAM-1 and VCAM-1 expression in HLSECs, which expressed CB(2) receptors, and the adhesion of human neutrophils to HLSECs in vitro. These findings suggest that selective CB(2) receptor agonists may represent a novel, protective strategy against I/R injury by attenuating oxidative stress, inflammatory response, and apoptosis. SN - 0741-5400 UR - https://www.unboundmedicine.com/medline/citation/17652447/Cannabinoid_2_receptor_agonist_HU_308_protects_against_hepatic_ischemia/reperfusion_injury_by_attenuating_oxidative_stress_inflammatory_response_and_apoptosis_ L2 - https://doi.org/10.1189/jlb.0307180 DB - PRIME DP - Unbound Medicine ER -