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Enhancement of intestinal absorption of macromolecules by spermine in rats.
Amino Acids. 2007 Aug; 33(2):253-60.AA

Abstract

The aim of this study was to investigate the enhancing effect of polyamines on intestinal absorption of fluorescein isothiocyanate-labeled dextran (MW 4400, FD-4) in the in situ loop study and in vivo oral absorption study. Absorption of FD-4 from the jejunum was significantly enhanced by 5 mM spermine without serious membrane damage in the jejunum. An in vivo oral absorption study was also performed, and plasma FD-4 levels increased significantly after co-administration of 30 mM spermine. In the in vitro transport studies with Caco-2 cells, prolonged incubation with spermine resulted in a gradual decrease in transepithelial electrical resistance. This finding suggests that the absorption-enhancing mechanism of spermine partly includes opening the tight junctions of the epithelium via the paracellular route. These results indicate that excess oral ingestion of polyamines may have widespread health effects via the modulation of the intestinal epithelial barrier function.

Authors+Show Affiliations

Department of Clinical Dietetics and Human Nutrition, Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama, Japan. sugita@josai.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17653818

Citation

Sugita, Y, et al. "Enhancement of Intestinal Absorption of Macromolecules By Spermine in Rats." Amino Acids, vol. 33, no. 2, 2007, pp. 253-60.
Sugita Y, Takao K, Toyama Y, et al. Enhancement of intestinal absorption of macromolecules by spermine in rats. Amino Acids. 2007;33(2):253-60.
Sugita, Y., Takao, K., Toyama, Y., & Shirahata, A. (2007). Enhancement of intestinal absorption of macromolecules by spermine in rats. Amino Acids, 33(2), 253-60.
Sugita Y, et al. Enhancement of Intestinal Absorption of Macromolecules By Spermine in Rats. Amino Acids. 2007;33(2):253-60. PubMed PMID: 17653818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enhancement of intestinal absorption of macromolecules by spermine in rats. AU - Sugita,Y, AU - Takao,K, AU - Toyama,Y, AU - Shirahata,A, Y1 - 2007/07/30/ PY - 2006/11/22/received PY - 2007/02/01/accepted PY - 2007/7/27/pubmed PY - 2008/1/1/medline PY - 2007/7/27/entrez SP - 253 EP - 60 JF - Amino acids JO - Amino Acids VL - 33 IS - 2 N2 - The aim of this study was to investigate the enhancing effect of polyamines on intestinal absorption of fluorescein isothiocyanate-labeled dextran (MW 4400, FD-4) in the in situ loop study and in vivo oral absorption study. Absorption of FD-4 from the jejunum was significantly enhanced by 5 mM spermine without serious membrane damage in the jejunum. An in vivo oral absorption study was also performed, and plasma FD-4 levels increased significantly after co-administration of 30 mM spermine. In the in vitro transport studies with Caco-2 cells, prolonged incubation with spermine resulted in a gradual decrease in transepithelial electrical resistance. This finding suggests that the absorption-enhancing mechanism of spermine partly includes opening the tight junctions of the epithelium via the paracellular route. These results indicate that excess oral ingestion of polyamines may have widespread health effects via the modulation of the intestinal epithelial barrier function. SN - 1438-2199 UR - https://www.unboundmedicine.com/medline/citation/17653818/Enhancement_of_intestinal_absorption_of_macromolecules_by_spermine_in_rats_ L2 - https://dx.doi.org/10.1007/s00726-007-0532-1 DB - PRIME DP - Unbound Medicine ER -