Tags

Type your tag names separated by a space and hit enter

Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing.
Forensic Sci Int. 2007 Aug 06; 170(2-3):156-62.FS

Abstract

The aim of this study was to assess the effectiveness of two current on-site oral fluid (OF) drug detection devices (OraLab and Dräger), as part of the Spanish participation in the Roadside Testing Assessment Project (ROSITA Project). The study was done in collaboration with the Spanish Traffic Police, in Galicia (NW Spain), during 2004 and 2005. A total of 468 drivers selected at the police controls agreed to participate through informed consent. In addition, saliva samples were collected and sent to the laboratory to confirm the on-site results. For this purpose, two different analytical liquid chromatography-mass spectrometry (LC-MS) methods were used to detect 11 drugs or metabolites in a 300 microL sample. Simultaneous analysis of morphine, 6-acetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, cocaine and benzoylecgonine was carried out using 100 microL of oral fluid, after an automated solid phase extraction. A different LC-MS method was performed to detect Delta(9)-THC in 200 microL of oral fluid using liquid-liquid extraction with hexane at pH 6. Both methods were fully validated, including linearity (1-250 ng/mL, 2-250 ng/mL) recovery (>50%), within-day and between-day precision (CV<15%), accuracy (mean relative error<15%), limit of detection (0.5 and 1 ng/mL), quantitation (1 and 2 ng/mL) and matrix effect. All of the positive cases and a random selection of 30% of the negatives were analyzed for confirmation analysis. Good results (sensitivity, specificity, accuracy, positive predictive value and negative predictive value>90%) were obtained for cocaine and opiates by OraLab, and for cocaine by Dräger. However, the results for the other compounds could be improved for both detection devices. Differences in the ease of use and in the interpretation mode (visual or instrumental) were observed.

Authors+Show Affiliations

Forensic Toxicology Service, Institute of Legal Medicine, University of Santiago de Compostela, Santiago de Compostela, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

17658709

Citation

Concheiro, Marta, et al. "Confirmation By LC-MS of Drugs in Oral Fluid Obtained From Roadside Testing." Forensic Science International, vol. 170, no. 2-3, 2007, pp. 156-62.
Concheiro M, de Castro A, Quintela O, et al. Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing. Forensic Sci Int. 2007;170(2-3):156-62.
Concheiro, M., de Castro, A., Quintela, O., Cruz, A., & López-Rivadulla, M. (2007). Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing. Forensic Science International, 170(2-3), 156-62.
Concheiro M, et al. Confirmation By LC-MS of Drugs in Oral Fluid Obtained From Roadside Testing. Forensic Sci Int. 2007 Aug 6;170(2-3):156-62. PubMed PMID: 17658709.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Confirmation by LC-MS of drugs in oral fluid obtained from roadside testing. AU - Concheiro,Marta, AU - de Castro,Ana, AU - Quintela,Oscar, AU - Cruz,Angelines, AU - López-Rivadulla,Manuel, Y1 - 2007/07/20/ PY - 2006/09/20/received PY - 2007/03/06/revised PY - 2007/03/06/accepted PY - 2007/7/31/pubmed PY - 2007/10/19/medline PY - 2007/7/31/entrez SP - 156 EP - 62 JF - Forensic science international JO - Forensic Sci Int VL - 170 IS - 2-3 N2 - The aim of this study was to assess the effectiveness of two current on-site oral fluid (OF) drug detection devices (OraLab and Dräger), as part of the Spanish participation in the Roadside Testing Assessment Project (ROSITA Project). The study was done in collaboration with the Spanish Traffic Police, in Galicia (NW Spain), during 2004 and 2005. A total of 468 drivers selected at the police controls agreed to participate through informed consent. In addition, saliva samples were collected and sent to the laboratory to confirm the on-site results. For this purpose, two different analytical liquid chromatography-mass spectrometry (LC-MS) methods were used to detect 11 drugs or metabolites in a 300 microL sample. Simultaneous analysis of morphine, 6-acetylmorphine, amphetamine, methamphetamine, MDA, MDMA, MDEA, MBDB, cocaine and benzoylecgonine was carried out using 100 microL of oral fluid, after an automated solid phase extraction. A different LC-MS method was performed to detect Delta(9)-THC in 200 microL of oral fluid using liquid-liquid extraction with hexane at pH 6. Both methods were fully validated, including linearity (1-250 ng/mL, 2-250 ng/mL) recovery (>50%), within-day and between-day precision (CV<15%), accuracy (mean relative error<15%), limit of detection (0.5 and 1 ng/mL), quantitation (1 and 2 ng/mL) and matrix effect. All of the positive cases and a random selection of 30% of the negatives were analyzed for confirmation analysis. Good results (sensitivity, specificity, accuracy, positive predictive value and negative predictive value>90%) were obtained for cocaine and opiates by OraLab, and for cocaine by Dräger. However, the results for the other compounds could be improved for both detection devices. Differences in the ease of use and in the interpretation mode (visual or instrumental) were observed. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/17658709/Confirmation_by_LC_MS_of_drugs_in_oral_fluid_obtained_from_roadside_testing_ DB - PRIME DP - Unbound Medicine ER -