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Staphylococcus aureus skin colonization in atopic dermatitis children is associated with decreased IFN-gamma production by peripheral blood CD4+ and CD8+ T cells.
Pediatr Allergy Immunol. 2008 Feb; 19(1):37-45.PA

Abstract

Atopic dermatitis (AD) is a chronic inflammatory skin disorder, which is associated with an increased expression of Th2 cytokines with concomitant decrease in IFN-gamma production by circulating CD4+ and CD8+ T cells. The skin of patients with AD is often colonized by Staphylococcus aureus, which may reflect in changes in immunological parameters. The aim of the study was flow cytometric measurement of some peripheral blood lymphocyte subsets expressing naive/memory marker (RA/RO) and activation marker (CD25) as well as intracellular production of IFN-gamma by peripheral blood CD4+ and CD8+ T cells from varied severity AD children and determine the impact of S. aureus skin colonization on cytokines profiles. There was a significant increase in the percentage of CD4+ and CD8+ T cells producing IL-4 and IL-13 and decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma upon in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin in children with AD compared to healthy ones. The absolute number of CD4+ and CD8+ T cells expressing memory marker CD45RO was elevated as compared with controls. The severity of AD was positively correlated with the percentage of lymphocyte subsets: CD45RO+, CD4+CD45RO+, and the percentage of CD3+ and CD4+ expressing CD25 as well as the number of S. aureus on the skin. In conclusion, both CD4+ and CD8+ memory T cells are involved in the immunopathogenesis of AD. S. aureus skin colonization is related with disease severity and changes in expression of CD45RO and CD25 on T cells. A decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma in AD children may explain propensity for skin infection.

Authors+Show Affiliations

Chair and Department of Pediatric Gastroenterology, Allergology and Developmental Disorders, Medical University of Silesia, Zabrze, Poland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17662038

Citation

Machura, Edyta, et al. "Staphylococcus Aureus Skin Colonization in Atopic Dermatitis Children Is Associated With Decreased IFN-gamma Production By Peripheral Blood CD4+ and CD8+ T Cells." Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, vol. 19, no. 1, 2008, pp. 37-45.
Machura E, Mazur B, Golemiec E, et al. Staphylococcus aureus skin colonization in atopic dermatitis children is associated with decreased IFN-gamma production by peripheral blood CD4+ and CD8+ T cells. Pediatr Allergy Immunol. 2008;19(1):37-45.
Machura, E., Mazur, B., Golemiec, E., Pindel, M., & Halkiewicz, F. (2008). Staphylococcus aureus skin colonization in atopic dermatitis children is associated with decreased IFN-gamma production by peripheral blood CD4+ and CD8+ T cells. Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, 19(1), 37-45.
Machura E, et al. Staphylococcus Aureus Skin Colonization in Atopic Dermatitis Children Is Associated With Decreased IFN-gamma Production By Peripheral Blood CD4+ and CD8+ T Cells. Pediatr Allergy Immunol. 2008;19(1):37-45. PubMed PMID: 17662038.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Staphylococcus aureus skin colonization in atopic dermatitis children is associated with decreased IFN-gamma production by peripheral blood CD4+ and CD8+ T cells. AU - Machura,Edyta, AU - Mazur,Bogdan, AU - Golemiec,Ewa, AU - Pindel,Mariola, AU - Halkiewicz,Franciszek, Y1 - 2007/07/27/ PY - 2007/7/31/pubmed PY - 2008/4/30/medline PY - 2007/7/31/entrez SP - 37 EP - 45 JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology JO - Pediatr Allergy Immunol VL - 19 IS - 1 N2 - Atopic dermatitis (AD) is a chronic inflammatory skin disorder, which is associated with an increased expression of Th2 cytokines with concomitant decrease in IFN-gamma production by circulating CD4+ and CD8+ T cells. The skin of patients with AD is often colonized by Staphylococcus aureus, which may reflect in changes in immunological parameters. The aim of the study was flow cytometric measurement of some peripheral blood lymphocyte subsets expressing naive/memory marker (RA/RO) and activation marker (CD25) as well as intracellular production of IFN-gamma by peripheral blood CD4+ and CD8+ T cells from varied severity AD children and determine the impact of S. aureus skin colonization on cytokines profiles. There was a significant increase in the percentage of CD4+ and CD8+ T cells producing IL-4 and IL-13 and decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma upon in vitro stimulation with phorbol 12-myristate 13-acetate and ionomycin in children with AD compared to healthy ones. The absolute number of CD4+ and CD8+ T cells expressing memory marker CD45RO was elevated as compared with controls. The severity of AD was positively correlated with the percentage of lymphocyte subsets: CD45RO+, CD4+CD45RO+, and the percentage of CD3+ and CD4+ expressing CD25 as well as the number of S. aureus on the skin. In conclusion, both CD4+ and CD8+ memory T cells are involved in the immunopathogenesis of AD. S. aureus skin colonization is related with disease severity and changes in expression of CD45RO and CD25 on T cells. A decrease in the percentage of CD4+ and CD8+ T cells producing IFN-gamma in AD children may explain propensity for skin infection. SN - 0905-6157 UR - https://www.unboundmedicine.com/medline/citation/17662038/Staphylococcus_aureus_skin_colonization_in_atopic_dermatitis_children_is_associated_with_decreased_IFN_gamma_production_by_peripheral_blood_CD4+_and_CD8+_T_cells_ L2 - https://doi.org/10.1111/j.1399-3038.2007.00586.x DB - PRIME DP - Unbound Medicine ER -