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The effects of fish oil and high or low linoleic acid intake on fatty acid composition of human peripheral blood mononuclear cells.
Br J Nutr. 2008 Jan; 99(1):147-54.BJ

Abstract

Dietary intake of 18: 2n-6 and 18: 3n-3 may affect endogenous production and incorporation of n-3 long-chain PUFA (LCPUFA) from fish oils (FO). This double-blinded controlled 2 x 2-factorial 8-week intervention investigates the effects of high and low 18: 2n-6 intake in combination with FO-supplementation on tissue fatty acid composition. Healthy young men (n 64) were randomized to capsules with FO or olive oil (control) (4.4 (2.0-5.6) ml/d) and to either sunflower oil and margarine (S/B) or rapeseed oil and a butter spread (R/K) to provide a high or a low 18: 2n-6 intake. Diet was measured by 4-d weighed dietary records at baseline, during and 8 weeks after the intervention and tissue incorporation as fatty acid composition of peripheral blood mononuclear cells (PBMC). The fat intervention gave a mean difference in the 18: 2n-6 intake of 7.3 g/d (95 % CI 4.6, 10.0) and a similar 18: 3n-3 intake in the groups. The R/K groups had a 0.2 % fatty acid (FA%) (95 % CI 0.0, 0.4, P = 0.02) higher content of 22: 5n-3 in the PBMC, a tendency of slightly higher 20: 5n-3 (P = 0.06), but no more 22: 6n-3 (P = 0.83) than the S/B groups. FO effectively raised the PBMC content of all n-3 LCPUFA (P < 0.001). The fat intervention did not markedly influence the effect of FO; the mean PBMC content of n-3 LCPUFA was 10.3 (sem 0.3) FA% in the FO+S/B group and 10.6 (sem 0.2) FA% in the FO+R/K group. In conclusion, increasing the 18: 2n-6 intake did not have any pronounced effect on incorporation of n-3 LCPUFA in PBMC, either alone or with simultaneous FO supplementation.

Authors+Show Affiliations

Department of Human Nutrition, Faculty of Life Sciences, Copenhagen University, DK-1958 Frederiksberg C, Denmark. ctd@life.ku.dkNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17663804

Citation

Damsgaard, Camilla T., et al. "The Effects of Fish Oil and High or Low Linoleic Acid Intake On Fatty Acid Composition of Human Peripheral Blood Mononuclear Cells." The British Journal of Nutrition, vol. 99, no. 1, 2008, pp. 147-54.
Damsgaard CT, Frøkiaer H, Lauritzen L. The effects of fish oil and high or low linoleic acid intake on fatty acid composition of human peripheral blood mononuclear cells. Br J Nutr. 2008;99(1):147-54.
Damsgaard, C. T., Frøkiaer, H., & Lauritzen, L. (2008). The effects of fish oil and high or low linoleic acid intake on fatty acid composition of human peripheral blood mononuclear cells. The British Journal of Nutrition, 99(1), 147-54.
Damsgaard CT, Frøkiaer H, Lauritzen L. The Effects of Fish Oil and High or Low Linoleic Acid Intake On Fatty Acid Composition of Human Peripheral Blood Mononuclear Cells. Br J Nutr. 2008;99(1):147-54. PubMed PMID: 17663804.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of fish oil and high or low linoleic acid intake on fatty acid composition of human peripheral blood mononuclear cells. AU - Damsgaard,Camilla T, AU - Frøkiaer,Hanne, AU - Lauritzen,Lotte, Y1 - 2007/07/30/ PY - 2007/8/1/pubmed PY - 2008/5/17/medline PY - 2007/8/1/entrez SP - 147 EP - 54 JF - The British journal of nutrition JO - Br J Nutr VL - 99 IS - 1 N2 - Dietary intake of 18: 2n-6 and 18: 3n-3 may affect endogenous production and incorporation of n-3 long-chain PUFA (LCPUFA) from fish oils (FO). This double-blinded controlled 2 x 2-factorial 8-week intervention investigates the effects of high and low 18: 2n-6 intake in combination with FO-supplementation on tissue fatty acid composition. Healthy young men (n 64) were randomized to capsules with FO or olive oil (control) (4.4 (2.0-5.6) ml/d) and to either sunflower oil and margarine (S/B) or rapeseed oil and a butter spread (R/K) to provide a high or a low 18: 2n-6 intake. Diet was measured by 4-d weighed dietary records at baseline, during and 8 weeks after the intervention and tissue incorporation as fatty acid composition of peripheral blood mononuclear cells (PBMC). The fat intervention gave a mean difference in the 18: 2n-6 intake of 7.3 g/d (95 % CI 4.6, 10.0) and a similar 18: 3n-3 intake in the groups. The R/K groups had a 0.2 % fatty acid (FA%) (95 % CI 0.0, 0.4, P = 0.02) higher content of 22: 5n-3 in the PBMC, a tendency of slightly higher 20: 5n-3 (P = 0.06), but no more 22: 6n-3 (P = 0.83) than the S/B groups. FO effectively raised the PBMC content of all n-3 LCPUFA (P < 0.001). The fat intervention did not markedly influence the effect of FO; the mean PBMC content of n-3 LCPUFA was 10.3 (sem 0.3) FA% in the FO+S/B group and 10.6 (sem 0.2) FA% in the FO+R/K group. In conclusion, increasing the 18: 2n-6 intake did not have any pronounced effect on incorporation of n-3 LCPUFA in PBMC, either alone or with simultaneous FO supplementation. SN - 0007-1145 UR - https://www.unboundmedicine.com/medline/citation/17663804/The_effects_of_fish_oil_and_high_or_low_linoleic_acid_intake_on_fatty_acid_composition_of_human_peripheral_blood_mononuclear_cells_ DB - PRIME DP - Unbound Medicine ER -