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Beneficial effects of FeTSPP, a peroxynitrite decomposition catalyst, in a mouse model of spinal cord injury.
Free Radic Biol Med. 2007 Sep 01; 43(5):763-80.FR

Abstract

The aim of the present study was to assess the contribution of peroxynitrite formation in the pathophysiology of spinal cord injury (SCI) in mice. To this purpose, we used a peroxynitrite decomposition catalyst, 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron III chloride (FeTSPP). Spinal cord trauma was induced by the application of vascular clips (force of 24g) to the dura via a four-level T5-T8 laminectomy. SCI in mice resulted in severe trauma characterized by edema, neutrophil infiltration, production of inflammatory mediators, tissue damage, and apoptosis. FeTSPP treatment (10-100 mg/kg, i.p.) significantly reduced in dose-dependent manner 1 and 4 h after the SCI (1) the degree of spinal cord inflammation and tissue injury (histological score), (2) neutrophil infiltration (myeloperoxidase activity), (3) nitrotyrosine formation and poly-(ADP-ribose) polymerase activation, (4) proinflammmaory cytokines expression, (5) NF-kappaB activation, and (6) apoptosis (TUNEL staining, Bax and Bcl-2 expression). Moreover, FeTSPP significantly ameliorated the recovery of limb function (evaluated by motor recovery score) in a dose-dependent manner. Taken together, our results clearly demonstrate that FeTSPP treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma similarly to dexamethasone, a well-known antiinflammatory agent which we have used as positive control.

Authors+Show Affiliations

Department of Clinical and Experimental Medicine and Pharmacology, School of Medicine, University of Messina, 98100 Messina, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17664140

Citation

Genovese, Tiziana, et al. "Beneficial Effects of FeTSPP, a Peroxynitrite Decomposition Catalyst, in a Mouse Model of Spinal Cord Injury." Free Radical Biology & Medicine, vol. 43, no. 5, 2007, pp. 763-80.
Genovese T, Mazzon E, Esposito E, et al. Beneficial effects of FeTSPP, a peroxynitrite decomposition catalyst, in a mouse model of spinal cord injury. Free Radic Biol Med. 2007;43(5):763-80.
Genovese, T., Mazzon, E., Esposito, E., Muià, C., Di Paola, R., Bramanti, P., & Cuzzocrea, S. (2007). Beneficial effects of FeTSPP, a peroxynitrite decomposition catalyst, in a mouse model of spinal cord injury. Free Radical Biology & Medicine, 43(5), 763-80.
Genovese T, et al. Beneficial Effects of FeTSPP, a Peroxynitrite Decomposition Catalyst, in a Mouse Model of Spinal Cord Injury. Free Radic Biol Med. 2007 Sep 1;43(5):763-80. PubMed PMID: 17664140.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Beneficial effects of FeTSPP, a peroxynitrite decomposition catalyst, in a mouse model of spinal cord injury. AU - Genovese,Tiziana, AU - Mazzon,Emanuela, AU - Esposito,Emanuela, AU - Muià,Carmelo, AU - Di Paola,Rosanna, AU - Bramanti,Placido, AU - Cuzzocrea,Salvatore, Y1 - 2007/05/16/ PY - 2007/03/01/received PY - 2007/05/08/revised PY - 2007/05/10/accepted PY - 2007/8/1/pubmed PY - 2007/10/27/medline PY - 2007/8/1/entrez SP - 763 EP - 80 JF - Free radical biology & medicine JO - Free Radic Biol Med VL - 43 IS - 5 N2 - The aim of the present study was to assess the contribution of peroxynitrite formation in the pathophysiology of spinal cord injury (SCI) in mice. To this purpose, we used a peroxynitrite decomposition catalyst, 5,10,15,20-tetrakis(4-sulfonatophenyl)porphyrinato iron III chloride (FeTSPP). Spinal cord trauma was induced by the application of vascular clips (force of 24g) to the dura via a four-level T5-T8 laminectomy. SCI in mice resulted in severe trauma characterized by edema, neutrophil infiltration, production of inflammatory mediators, tissue damage, and apoptosis. FeTSPP treatment (10-100 mg/kg, i.p.) significantly reduced in dose-dependent manner 1 and 4 h after the SCI (1) the degree of spinal cord inflammation and tissue injury (histological score), (2) neutrophil infiltration (myeloperoxidase activity), (3) nitrotyrosine formation and poly-(ADP-ribose) polymerase activation, (4) proinflammmaory cytokines expression, (5) NF-kappaB activation, and (6) apoptosis (TUNEL staining, Bax and Bcl-2 expression). Moreover, FeTSPP significantly ameliorated the recovery of limb function (evaluated by motor recovery score) in a dose-dependent manner. Taken together, our results clearly demonstrate that FeTSPP treatment reduces the development of inflammation and tissue injury associated with spinal cord trauma similarly to dexamethasone, a well-known antiinflammatory agent which we have used as positive control. SN - 0891-5849 UR - https://www.unboundmedicine.com/medline/citation/17664140/Beneficial_effects_of_FeTSPP_a_peroxynitrite_decomposition_catalyst_in_a_mouse_model_of_spinal_cord_injury_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(07)00329-2 DB - PRIME DP - Unbound Medicine ER -