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Phototoxicity of bergamot oil assessed by in vitro techniques in combination with human patch tests.
Toxicol In Vitro 2007; 21(7):1298-303TV

Abstract

The aim of this study was to clarify the differences in the phototoxicity of bergamot oil obtained from four different suppliers. Spectral and chemical analyses were performed to identify presence of photoactive compounds in the test samples. The phototoxicity was assessed in vitro by the 3T3 NRU phototoxicity test (PT) and subsequently in a phototoxicity test on reconstructed human skin model (H3D PT). Confirmatory photopatch tests in a group of volunteers were performed using the first non-phototoxic concentration determined in the H3D PT. The spectral and chemical analyses revealed, that two samples of bergamot oil exhibited a potential for photoactivation. These oils were subsequently classified as phototoxic in the 3T3 NRU PT, however, only on the basis of borderline results and depending on the solvent used. H3D PT revealed clear classifications, correlating well with the findings of spectral and chemical analysis. The test was, however, not yet capable of precise prediction of safe, non-phototoxic concentrations. Additional endpoints, e.g. interleukin determination might be employed to increase the sensitivity of the test. Although the study showed the usefulness of the tiered testing strategy, currently, the extrapolation of in vitro results to human situation may be performed only to a limited extent.

Authors+Show Affiliations

National Reference Center for Cosmetics, National Institute of Public Health, Srobárova 48, 100 42 Prague 10, Czech Republic. kejlova@szu.czNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17669618

Citation

Kejlová, K, et al. "Phototoxicity of Bergamot Oil Assessed By in Vitro Techniques in Combination With Human Patch Tests." Toxicology in Vitro : an International Journal Published in Association With BIBRA, vol. 21, no. 7, 2007, pp. 1298-303.
Kejlová K, Jírová D, Bendová H, et al. Phototoxicity of bergamot oil assessed by in vitro techniques in combination with human patch tests. Toxicol In Vitro. 2007;21(7):1298-303.
Kejlová, K., Jírová, D., Bendová, H., Kandárová, H., Weidenhoffer, Z., Kolárová, H., & Liebsch, M. (2007). Phototoxicity of bergamot oil assessed by in vitro techniques in combination with human patch tests. Toxicology in Vitro : an International Journal Published in Association With BIBRA, 21(7), pp. 1298-303.
Kejlová K, et al. Phototoxicity of Bergamot Oil Assessed By in Vitro Techniques in Combination With Human Patch Tests. Toxicol In Vitro. 2007;21(7):1298-303. PubMed PMID: 17669618.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phototoxicity of bergamot oil assessed by in vitro techniques in combination with human patch tests. AU - Kejlová,K, AU - Jírová,D, AU - Bendová,H, AU - Kandárová,H, AU - Weidenhoffer,Z, AU - Kolárová,H, AU - Liebsch,M, Y1 - 2007/06/13/ PY - 2006/09/29/received PY - 2007/05/29/accepted PY - 2007/8/3/pubmed PY - 2008/2/6/medline PY - 2007/8/3/entrez SP - 1298 EP - 303 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 21 IS - 7 N2 - The aim of this study was to clarify the differences in the phototoxicity of bergamot oil obtained from four different suppliers. Spectral and chemical analyses were performed to identify presence of photoactive compounds in the test samples. The phototoxicity was assessed in vitro by the 3T3 NRU phototoxicity test (PT) and subsequently in a phototoxicity test on reconstructed human skin model (H3D PT). Confirmatory photopatch tests in a group of volunteers were performed using the first non-phototoxic concentration determined in the H3D PT. The spectral and chemical analyses revealed, that two samples of bergamot oil exhibited a potential for photoactivation. These oils were subsequently classified as phototoxic in the 3T3 NRU PT, however, only on the basis of borderline results and depending on the solvent used. H3D PT revealed clear classifications, correlating well with the findings of spectral and chemical analysis. The test was, however, not yet capable of precise prediction of safe, non-phototoxic concentrations. Additional endpoints, e.g. interleukin determination might be employed to increase the sensitivity of the test. Although the study showed the usefulness of the tiered testing strategy, currently, the extrapolation of in vitro results to human situation may be performed only to a limited extent. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/17669618/Phototoxicity_of_bergamot_oil_assessed_by_in_vitro_techniques_in_combination_with_human_patch_tests L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(07)00167-1 DB - PRIME DP - Unbound Medicine ER -