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High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF".
Rev Sci Instrum. 2007 Jul; 78(7):074101.RS

Abstract

A new multiturn tandem time-of-flight (TOF) mass spectrometer "MULTUM-TOF/TOF" has been designed and constructed. It consists of a matrix-assisted laser desorption/ionization ion source, a multiturn TOF mass spectrometer, a collision cell, and a quadratic-field ion mirror. The multiturn TOF mass spectrometer can overcome the problem of precursor ion selection in TOF, due to insufficient time separation between two adjacent TOF peaks, by increasing the number of cycles. As a result, the total TOF increases with the increase in resolving power. The quadratic-field ion mirror allows temporal focusing for fragment ions with different kinetic energies. Product ion spectra from monoisotopically selected precursor ions of angiotensin I, substance P, and bradykinin have been obtained. The fragment ions observed are mainly the result of high-energy collision induced dissociation.

Authors+Show Affiliations

Department of Physics, Graduate School of Science, Osaka University, 1-1 Machikaneyama, Toyonaka, Osaka 560-0043, Japan. toyodam@phys.sci.osaka-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17672775

Citation

Toyoda, Michisato, et al. "High-energy Collision Induced Dissociation Fragmentation Pathways of Peptides, Probed Using a Multiturn Tandem Time-of-flight Mass Spectrometer "MULTUM-TOF/TOF"." The Review of Scientific Instruments, vol. 78, no. 7, 2007, p. 074101.
Toyoda M, Giannakopulos AE, Colburn AW, et al. High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF". Rev Sci Instrum. 2007;78(7):074101.
Toyoda, M., Giannakopulos, A. E., Colburn, A. W., & Derrick, P. J. (2007). High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF". The Review of Scientific Instruments, 78(7), 074101.
Toyoda M, et al. High-energy Collision Induced Dissociation Fragmentation Pathways of Peptides, Probed Using a Multiturn Tandem Time-of-flight Mass Spectrometer "MULTUM-TOF/TOF". Rev Sci Instrum. 2007;78(7):074101. PubMed PMID: 17672775.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-energy collision induced dissociation fragmentation pathways of peptides, probed using a multiturn tandem time-of-flight mass spectrometer "MULTUM-TOF/TOF". AU - Toyoda,Michisato, AU - Giannakopulos,Anastassios E, AU - Colburn,Alex W, AU - Derrick,Peter J, PY - 2007/8/4/pubmed PY - 2007/10/20/medline PY - 2007/8/4/entrez SP - 074101 EP - 074101 JF - The Review of scientific instruments JO - Rev Sci Instrum VL - 78 IS - 7 N2 - A new multiturn tandem time-of-flight (TOF) mass spectrometer "MULTUM-TOF/TOF" has been designed and constructed. It consists of a matrix-assisted laser desorption/ionization ion source, a multiturn TOF mass spectrometer, a collision cell, and a quadratic-field ion mirror. The multiturn TOF mass spectrometer can overcome the problem of precursor ion selection in TOF, due to insufficient time separation between two adjacent TOF peaks, by increasing the number of cycles. As a result, the total TOF increases with the increase in resolving power. The quadratic-field ion mirror allows temporal focusing for fragment ions with different kinetic energies. Product ion spectra from monoisotopically selected precursor ions of angiotensin I, substance P, and bradykinin have been obtained. The fragment ions observed are mainly the result of high-energy collision induced dissociation. SN - 0034-6748 UR - https://www.unboundmedicine.com/medline/citation/17672775/High_energy_collision_induced_dissociation_fragmentation_pathways_of_peptides_probed_using_a_multiturn_tandem_time_of_flight_mass_spectrometer_"MULTUM_TOF/TOF"_ L2 - https://doi.org/10.1063/1.2751403 DB - PRIME DP - Unbound Medicine ER -