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Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL.
Arch Toxicol. 2007 Oct; 81(10):719-28.AT

Abstract

Berberine is the major constituent of Coptidis Rhizoma with multiple pharmacological activities, including anti-inflammation, promotion of apoptosis and anticancer potential effect. Mitogen-activated protein kinase (MAPK) and reactive oxygen species (ROS) may contribute to the causal relationship between tumorigenesis and pro-apoptotic function. Berberine is studied for the mechanism of its action in apoptotic pathway in human colonic carcinoma cell. Treatment of SW620 cells with 50 microM berberine resulted in activation of the caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c; whereas, the expression of BID and anti-apoptosis factor c-IAP1, Bcl-2, and Bcl-(XL) were decreased markedly. Berberine-induced, dose-dependent induction of apoptosis was accompanied by sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Furthermore, the induction of apoptosis was alleviated by inhibitors specific for JNK and p38. In addition, there was an increase in the cellular levels of phospho-c-Jun, FasL and t-BID in the berberine-induced apoptosis via the activation of JNK and p38 signaling modules. NAC administration, a scavenger of ROS, reversed berberine-induced apoptosis effects via inhibition of JNK, p38 and c-jun activation, and FasL and t-BID expression. These results leads us to speculate that berberine may play an apoptotic cascade in SW620 cells by activation of the JNK/p38 pathway and induction of ROS production, providing a new mechanism for berberine-induced cell death in human colon cancer cells.

Authors+Show Affiliations

Division of Gastroenterology, Department of Internal Medicine, Armed-Forces Taichung General Hospital, No. 348, Section 2, Chung Shan Road, Taiping City, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17673978

Citation

Hsu, Wen-Hsiu, et al. "Berberine Induces Apoptosis in SW620 Human Colonic Carcinoma Cells Through Generation of Reactive Oxygen Species and Activation of JNK/p38 MAPK and FasL." Archives of Toxicology, vol. 81, no. 10, 2007, pp. 719-28.
Hsu WH, Hsieh YS, Kuo HC, et al. Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. Arch Toxicol. 2007;81(10):719-28.
Hsu, W. H., Hsieh, Y. S., Kuo, H. C., Teng, C. Y., Huang, H. I., Wang, C. J., Yang, S. F., Liou, Y. S., & Kuo, W. H. (2007). Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. Archives of Toxicology, 81(10), 719-28.
Hsu WH, et al. Berberine Induces Apoptosis in SW620 Human Colonic Carcinoma Cells Through Generation of Reactive Oxygen Species and Activation of JNK/p38 MAPK and FasL. Arch Toxicol. 2007;81(10):719-28. PubMed PMID: 17673978.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Berberine induces apoptosis in SW620 human colonic carcinoma cells through generation of reactive oxygen species and activation of JNK/p38 MAPK and FasL. AU - Hsu,Wen-Hsiu, AU - Hsieh,Yih-Shou, AU - Kuo,Hsing-Chun, AU - Teng,Chun-Yuh, AU - Huang,Hai-I, AU - Wang,Chau-Jong, AU - Yang,Shun-Fa, AU - Liou,Yi-Sheng, AU - Kuo,Wu-Hsien, Y1 - 2007/08/03/ PY - 2006/06/25/received PY - 2006/11/09/accepted PY - 2007/8/4/pubmed PY - 2008/2/1/medline PY - 2007/8/4/entrez SP - 719 EP - 28 JF - Archives of toxicology JO - Arch Toxicol VL - 81 IS - 10 N2 - Berberine is the major constituent of Coptidis Rhizoma with multiple pharmacological activities, including anti-inflammation, promotion of apoptosis and anticancer potential effect. Mitogen-activated protein kinase (MAPK) and reactive oxygen species (ROS) may contribute to the causal relationship between tumorigenesis and pro-apoptotic function. Berberine is studied for the mechanism of its action in apoptotic pathway in human colonic carcinoma cell. Treatment of SW620 cells with 50 microM berberine resulted in activation of the caspase 3 and caspase 8, cleavage of poly ADP-ribose polymerase (PARP) and the release of cytochrome c; whereas, the expression of BID and anti-apoptosis factor c-IAP1, Bcl-2, and Bcl-(XL) were decreased markedly. Berberine-induced, dose-dependent induction of apoptosis was accompanied by sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Furthermore, the induction of apoptosis was alleviated by inhibitors specific for JNK and p38. In addition, there was an increase in the cellular levels of phospho-c-Jun, FasL and t-BID in the berberine-induced apoptosis via the activation of JNK and p38 signaling modules. NAC administration, a scavenger of ROS, reversed berberine-induced apoptosis effects via inhibition of JNK, p38 and c-jun activation, and FasL and t-BID expression. These results leads us to speculate that berberine may play an apoptotic cascade in SW620 cells by activation of the JNK/p38 pathway and induction of ROS production, providing a new mechanism for berberine-induced cell death in human colon cancer cells. SN - 0340-5761 UR - https://www.unboundmedicine.com/medline/citation/17673978/Berberine_induces_apoptosis_in_SW620_human_colonic_carcinoma_cells_through_generation_of_reactive_oxygen_species_and_activation_of_JNK/p38_MAPK_and_FasL_ L2 - https://doi.org/10.1007/s00204-006-0169-y DB - PRIME DP - Unbound Medicine ER -