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In-column fiber-optic laser-induced fluorescence detection for CE.
Electrophoresis. 2007 Aug; 28(17):3105-14.E

Abstract

A highly sensitive in-column fiber-optic LIF detector for CE has been constructed and evaluated. In this detection system, a 457-nm diode-pumped solid-state blue laser was used as the excitation light source and an optical fiber (40 mum od) was used to transmit the excitation light. One end of the optical fiber was inserted into the separation capillary and was in situ positioned at the detection window. The other end of the fiber was protruded from the capillary to capture the excitation light beam from the blue laser. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a yellow color filter before reaching the photomultiplier tube. The present CE-fluorescence detection is a simple and compact optical system. It reduces the laser scattering effect from the capillary and fiber as compared to the conventional LIF detection for CE. Its utility was successfully demonstrated by the separation and determination of D-penicillamine labeled with naphthalene-2,3-dicarboxaldehyde. The detection limit was 0.8 nM (S/N = 3). The present detection scheme has been proven to be attractive for sensitive fluorescence detection for CE.

Authors+Show Affiliations

College of Chemistry, Sichuan University, Chengdu, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17674418

Citation

Yang, Xiupei, et al. "In-column Fiber-optic Laser-induced Fluorescence Detection for CE." Electrophoresis, vol. 28, no. 17, 2007, pp. 3105-14.
Yang X, Yuan H, Wang C, et al. In-column fiber-optic laser-induced fluorescence detection for CE. Electrophoresis. 2007;28(17):3105-14.
Yang, X., Yuan, H., Wang, C., Zhao, S., Xiao, D., & Choi, M. M. (2007). In-column fiber-optic laser-induced fluorescence detection for CE. Electrophoresis, 28(17), 3105-14.
Yang X, et al. In-column Fiber-optic Laser-induced Fluorescence Detection for CE. Electrophoresis. 2007;28(17):3105-14. PubMed PMID: 17674418.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-column fiber-optic laser-induced fluorescence detection for CE. AU - Yang,Xiupei, AU - Yuan,Hongyan, AU - Wang,Chunlin, AU - Zhao,Shulin, AU - Xiao,Dan, AU - Choi,Martin M F, PY - 2007/8/4/pubmed PY - 2007/12/19/medline PY - 2007/8/4/entrez SP - 3105 EP - 14 JF - Electrophoresis JO - Electrophoresis VL - 28 IS - 17 N2 - A highly sensitive in-column fiber-optic LIF detector for CE has been constructed and evaluated. In this detection system, a 457-nm diode-pumped solid-state blue laser was used as the excitation light source and an optical fiber (40 mum od) was used to transmit the excitation light. One end of the optical fiber was inserted into the separation capillary and was in situ positioned at the detection window. The other end of the fiber was protruded from the capillary to capture the excitation light beam from the blue laser. Fluorescence emission was collected by a 40 x microscope objective, focused on a spatial filter, and passed through a yellow color filter before reaching the photomultiplier tube. The present CE-fluorescence detection is a simple and compact optical system. It reduces the laser scattering effect from the capillary and fiber as compared to the conventional LIF detection for CE. Its utility was successfully demonstrated by the separation and determination of D-penicillamine labeled with naphthalene-2,3-dicarboxaldehyde. The detection limit was 0.8 nM (S/N = 3). The present detection scheme has been proven to be attractive for sensitive fluorescence detection for CE. SN - 0173-0835 UR - https://www.unboundmedicine.com/medline/citation/17674418/In_column_fiber_optic_laser_induced_fluorescence_detection_for_CE_ L2 - https://doi.org/10.1002/elps.200600815 DB - PRIME DP - Unbound Medicine ER -