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A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes.
Anal Biochem. 2007 Oct 01; 369(1):71-9.AB

Abstract

A novel glucose biosensor was constructed, based on the immobilization of glucose oxidase (GOx) with cross-linking in the matrix of biopolymer chitosan (CS) on a glassy carbon electrode (GCE), which was modified with gold-platinum alloy nanoparticles (Au-PtNPs) by electrodeposition on multiwall carbon nanotubes (CNTs) in CS film (CNTs/CS). The properties of Au-PtNPs/CNTs/CS were characterized by scan electron microscopy (SEM), X-ray diffraction (XRD), cyclic voltammetry (CV), and electrochemical impedance spectra (EIS). Primary study indicated that Au-PtNPs/CNTs had a better synergistic electrocatalytic effect on the reduction of hydrogen peroxide than did AuNPs/CNTs or PtNPs/CNTs at a low applied potential window. With GOx as a model enzyme, a new glucose biosensor was fabricated. The biosensor exhibited excellent performances for glucose at a low applied potential (0.1V) with a high sensitivity (8.53 microA mM(-1)), a low detection limit (0.2 microM), a wide linear range (0.001-7.0 mM), a fast response time (<5s), and good reproducibility, stability, and selectivity. In addition, the biosensor was applied in the determination of glucose in human blood and urine samples, and satisfied results were obtained.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou 510275, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17678866

Citation

Kang, Xinhuang, et al. "A Novel Glucose Biosensor Based On Immobilization of Glucose Oxidase in Chitosan On a Glassy Carbon Electrode Modified With Gold-platinum Alloy Nanoparticles/multiwall Carbon Nanotubes." Analytical Biochemistry, vol. 369, no. 1, 2007, pp. 71-9.
Kang X, Mai Z, Zou X, et al. A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes. Anal Biochem. 2007;369(1):71-9.
Kang, X., Mai, Z., Zou, X., Cai, P., & Mo, J. (2007). A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes. Analytical Biochemistry, 369(1), 71-9.
Kang X, et al. A Novel Glucose Biosensor Based On Immobilization of Glucose Oxidase in Chitosan On a Glassy Carbon Electrode Modified With Gold-platinum Alloy Nanoparticles/multiwall Carbon Nanotubes. Anal Biochem. 2007 Oct 1;369(1):71-9. PubMed PMID: 17678866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A novel glucose biosensor based on immobilization of glucose oxidase in chitosan on a glassy carbon electrode modified with gold-platinum alloy nanoparticles/multiwall carbon nanotubes. AU - Kang,Xinhuang, AU - Mai,Zhibin, AU - Zou,Xiaoyong, AU - Cai,Peixiang, AU - Mo,Jinyuan, Y1 - 2007/07/07/ PY - 2007/03/26/received PY - 2007/06/21/revised PY - 2007/07/02/accepted PY - 2007/8/7/pubmed PY - 2007/12/14/medline PY - 2007/8/7/entrez SP - 71 EP - 9 JF - Analytical biochemistry JO - Anal Biochem VL - 369 IS - 1 N2 - A novel glucose biosensor was constructed, based on the immobilization of glucose oxidase (GOx) with cross-linking in the matrix of biopolymer chitosan (CS) on a glassy carbon electrode (GCE), which was modified with gold-platinum alloy nanoparticles (Au-PtNPs) by electrodeposition on multiwall carbon nanotubes (CNTs) in CS film (CNTs/CS). The properties of Au-PtNPs/CNTs/CS were characterized by scan electron microscopy (SEM), X-ray diffraction (XRD), cyclic voltammetry (CV), and electrochemical impedance spectra (EIS). Primary study indicated that Au-PtNPs/CNTs had a better synergistic electrocatalytic effect on the reduction of hydrogen peroxide than did AuNPs/CNTs or PtNPs/CNTs at a low applied potential window. With GOx as a model enzyme, a new glucose biosensor was fabricated. The biosensor exhibited excellent performances for glucose at a low applied potential (0.1V) with a high sensitivity (8.53 microA mM(-1)), a low detection limit (0.2 microM), a wide linear range (0.001-7.0 mM), a fast response time (<5s), and good reproducibility, stability, and selectivity. In addition, the biosensor was applied in the determination of glucose in human blood and urine samples, and satisfied results were obtained. SN - 0003-2697 UR - https://www.unboundmedicine.com/medline/citation/17678866/A_novel_glucose_biosensor_based_on_immobilization_of_glucose_oxidase_in_chitosan_on_a_glassy_carbon_electrode_modified_with_gold_platinum_alloy_nanoparticles/multiwall_carbon_nanotubes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(07)00441-1 DB - PRIME DP - Unbound Medicine ER -