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Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis.
Fertil Steril. 2008 Feb; 89(2):301-10.FS

Abstract

OBJECTIVE

To examine messenger (m) RNA expression of aromatase, cytokines, and adhesion factors in women with and without endometriosis.

DESIGN

Patients with endometriosis were compared with control patients.

SETTING

University Hospital Gasthuisberg, Leuven, Belgium.

PATIENT(S)

A total of 35 patients who had laparoscopic surgery during the luteal phase (n = 20) or the menstrual phase (n = 15) were selected for this study based on cycle phase and presence/absence of endometriosis.

INTERVENTION(S)

Tissues of endometrium and macroscopically normal peritoneum were collected during hysteroscopy and laparoscopic surgery, respectively, from 24 women with revised American Society for Reproductive Medicine stage (rASRM) stages I-II (n = 12) and III-IV (n = 12) endometriosis and 11 control patients with normal pelvic. Tissue samples were selected from a tissue bank, based on the phase of the cycle (menstrual or luteal) and the presence/absence of endometriosis.

MAIN OUTCOME MEASURE(S)

The mRNA levels of aromatase, vimentin, vascular cell adhesion molecule 1 (VCAM-1), alpha(V) and beta(3) integrins, interleukin (IL)-1 beta, regulated on activation normal T-cell expressed and secreted (RANTES), and monocyte chemotactic protein 1 (MCP-1) were evaluated using real-time reverse transcriptase polymerase chain reaction.

RESULT(S)

During menstrual phase, increased endometrial mRNA levels of alpha(V) integrin, combined alpha(V)beta(3) integrins, and increased peritoneal IL-1 beta mRNA levels--but decreased peritoneal MCP-1 mRNA levels--were observed in women with endometriosis compared with control subjects. During luteal phase, endometrial mRNA levels of IL-1 beta and RANTES were increased in women with endometriosis compared with control subjects. Endometrial aromatase mRNA expression was higher in women with endometriosis than in control subjects in combined phases. Women with endometriosis had increased peritoneal mRNA expression of RANTES and VCAM-1 during menstrual compared with luteal phase.

CONCLUSION(S)

Aberrant mRNA expression of aromatase, cytokines, and adhesion factors in endometrium and peritoneum suggests that both tissues are involved in the pathogenesis of endometriosis.

Authors+Show Affiliations

Leuven University Fertility Center, Department of Obstetrics and Gynecology, University Hospital Gasthuisberg, Leuven, Belgium.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17678915

Citation

Kyama, Cleophas M., et al. "Endometrial and Peritoneal Expression of Aromatase, Cytokines, and Adhesion Factors in Women With Endometriosis." Fertility and Sterility, vol. 89, no. 2, 2008, pp. 301-10.
Kyama CM, Overbergh L, Mihalyi A, et al. Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. Fertil Steril. 2008;89(2):301-10.
Kyama, C. M., Overbergh, L., Mihalyi, A., Meuleman, C., Mwenda, J. M., Mathieu, C., & D'Hooghe, T. M. (2008). Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. Fertility and Sterility, 89(2), 301-10.
Kyama CM, et al. Endometrial and Peritoneal Expression of Aromatase, Cytokines, and Adhesion Factors in Women With Endometriosis. Fertil Steril. 2008;89(2):301-10. PubMed PMID: 17678915.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Endometrial and peritoneal expression of aromatase, cytokines, and adhesion factors in women with endometriosis. AU - Kyama,Cleophas M, AU - Overbergh,Lutgart, AU - Mihalyi,Attila, AU - Meuleman,Christel, AU - Mwenda,Jason M, AU - Mathieu,Chantal, AU - D'Hooghe,Thomas M, Y1 - 2007/08/06/ PY - 2006/11/17/received PY - 2007/02/27/revised PY - 2007/02/27/accepted PY - 2007/8/7/pubmed PY - 2008/3/11/medline PY - 2007/8/7/entrez SP - 301 EP - 10 JF - Fertility and sterility JO - Fertil. Steril. VL - 89 IS - 2 N2 - OBJECTIVE: To examine messenger (m) RNA expression of aromatase, cytokines, and adhesion factors in women with and without endometriosis. DESIGN: Patients with endometriosis were compared with control patients. SETTING: University Hospital Gasthuisberg, Leuven, Belgium. PATIENT(S): A total of 35 patients who had laparoscopic surgery during the luteal phase (n = 20) or the menstrual phase (n = 15) were selected for this study based on cycle phase and presence/absence of endometriosis. INTERVENTION(S): Tissues of endometrium and macroscopically normal peritoneum were collected during hysteroscopy and laparoscopic surgery, respectively, from 24 women with revised American Society for Reproductive Medicine stage (rASRM) stages I-II (n = 12) and III-IV (n = 12) endometriosis and 11 control patients with normal pelvic. Tissue samples were selected from a tissue bank, based on the phase of the cycle (menstrual or luteal) and the presence/absence of endometriosis. MAIN OUTCOME MEASURE(S): The mRNA levels of aromatase, vimentin, vascular cell adhesion molecule 1 (VCAM-1), alpha(V) and beta(3) integrins, interleukin (IL)-1 beta, regulated on activation normal T-cell expressed and secreted (RANTES), and monocyte chemotactic protein 1 (MCP-1) were evaluated using real-time reverse transcriptase polymerase chain reaction. RESULT(S): During menstrual phase, increased endometrial mRNA levels of alpha(V) integrin, combined alpha(V)beta(3) integrins, and increased peritoneal IL-1 beta mRNA levels--but decreased peritoneal MCP-1 mRNA levels--were observed in women with endometriosis compared with control subjects. During luteal phase, endometrial mRNA levels of IL-1 beta and RANTES were increased in women with endometriosis compared with control subjects. Endometrial aromatase mRNA expression was higher in women with endometriosis than in control subjects in combined phases. Women with endometriosis had increased peritoneal mRNA expression of RANTES and VCAM-1 during menstrual compared with luteal phase. CONCLUSION(S): Aberrant mRNA expression of aromatase, cytokines, and adhesion factors in endometrium and peritoneum suggests that both tissues are involved in the pathogenesis of endometriosis. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17678915/Endometrial_and_peritoneal_expression_of_aromatase_cytokines_and_adhesion_factors_in_women_with_endometriosis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)00525-0 DB - PRIME DP - Unbound Medicine ER -