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Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots.
Nat Prod Res. 2007 Aug; 21(10):933-40.NP

Abstract

Root extracts of the plant Abrus precatorius L. was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography. The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to 4 chromatophores out of 7. The chromatophores were isolated from the TLC plates and rechecked for activity against Staphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity, i.e., 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity.

Authors+Show Affiliations

School of Life Sciences, SRTM University, Nanded (MS), India.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17680505

Citation

Zore, G B., et al. "Activity-directed Fractionation and Isolation of Four Antibacterial Compounds From Abrus Precatorius L. Roots." Natural Product Research, vol. 21, no. 10, 2007, pp. 933-40.
Zore GB, Awad V, Thakre AD, et al. Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots. Nat Prod Res. 2007;21(10):933-40.
Zore, G. B., Awad, V., Thakre, A. D., Halde, U. K., Meshram, N. S., Surwase, B. S., & Karuppayil, S. M. (2007). Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots. Natural Product Research, 21(10), 933-40.
Zore GB, et al. Activity-directed Fractionation and Isolation of Four Antibacterial Compounds From Abrus Precatorius L. Roots. Nat Prod Res. 2007;21(10):933-40. PubMed PMID: 17680505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Activity-directed fractionation and isolation of four antibacterial compounds from Abrus precatorius L. roots. AU - Zore,G B, AU - Awad,V, AU - Thakre,A D, AU - Halde,U K, AU - Meshram,N S, AU - Surwase,B S, AU - Karuppayil,S Mohan, PY - 2007/8/8/pubmed PY - 2007/11/1/medline PY - 2007/8/8/entrez SP - 933 EP - 40 JF - Natural product research JO - Nat Prod Res VL - 21 IS - 10 N2 - Root extracts of the plant Abrus precatorius L. was tested for antibacterial activity. Various solvent fractions exhibited inhibitory activity against 13 gram-positive and gram-negative bacteria. Root extracts were analyzed by thin layer chromatography. The antibacterial activity was localized to specific chromatophores in the chloroform fraction through a bioautography assay. It was found localized to 4 chromatophores out of 7. The chromatophores were isolated from the TLC plates and rechecked for activity against Staphylococcus aureus A, using a disc diffusion assay. Among the four active principles isolated, AP 3 (Rf 0.87) exhibited maximum activity, i.e., 56% inhibition of growth of S. aureus A, in disc diffusion assay compared to the standard antibiotic Ampicillin. Results of this study suggest that chloroform extractable phytochemicals in A. precatorius L. may yield promising molecules with antibiotic activity. SN - 1478-6419 UR - https://www.unboundmedicine.com/medline/citation/17680505/Activity_directed_fractionation_and_isolation_of_four_antibacterial_compounds_from_Abrus_precatorius_L__roots_ L2 - https://www.tandfonline.com/doi/full/10.1080/14786410701525992 DB - PRIME DP - Unbound Medicine ER -