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Major ampullate spidroins from Euprosthenops australis: multiplicity at protein, mRNA and gene levels.
Insect Mol Biol 2007; 16(5):551-61IM

Abstract

Spider dragline silk possesses extraordinary mechanical properties. It consists of large fibrous proteins called spidroins that display modular structures. It is known to consist of two proteins: the major ampullate spidroin (MaSp) 1 and MaSp2. This study analyses MaSp sequences from the nursery-web spider Euprosthenops australis. We have identified a previously uncharacterized MaSp2 sequence and a new MaSp-like spidroin, which display distinct homogenous submotifs within their respective Gly-rich repeats. Furthermore, a group of MaSp1 cDNA clones show unexpected heterogeneity. Genomic PCR identified several MaSp1 gene variants within individual spiders, which suggests the presence of a gene cluster in E. australis. Finally, the evolution of spidroin genes is discussed in relation to phylogenetic analysis of nonrepetitive C-terminal domains from diverse species.

Authors+Show Affiliations

Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Uppsala, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17680798

Citation

Rising, A, et al. "Major Ampullate Spidroins From Euprosthenops Australis: Multiplicity at Protein, mRNA and Gene Levels." Insect Molecular Biology, vol. 16, no. 5, 2007, pp. 551-61.
Rising A, Johansson J, Larson G, et al. Major ampullate spidroins from Euprosthenops australis: multiplicity at protein, mRNA and gene levels. Insect Mol Biol. 2007;16(5):551-61.
Rising, A., Johansson, J., Larson, G., Bongcam-Rudloff, E., Engström, W., & Hjälm, G. (2007). Major ampullate spidroins from Euprosthenops australis: multiplicity at protein, mRNA and gene levels. Insect Molecular Biology, 16(5), pp. 551-61.
Rising A, et al. Major Ampullate Spidroins From Euprosthenops Australis: Multiplicity at Protein, mRNA and Gene Levels. Insect Mol Biol. 2007;16(5):551-61. PubMed PMID: 17680798.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Major ampullate spidroins from Euprosthenops australis: multiplicity at protein, mRNA and gene levels. AU - Rising,A, AU - Johansson,J, AU - Larson,G, AU - Bongcam-Rudloff,E, AU - Engström,W, AU - Hjälm,G, Y1 - 2007/08/04/ PY - 2007/8/8/pubmed PY - 2007/12/14/medline PY - 2007/8/8/entrez SP - 551 EP - 61 JF - Insect molecular biology JO - Insect Mol. Biol. VL - 16 IS - 5 N2 - Spider dragline silk possesses extraordinary mechanical properties. It consists of large fibrous proteins called spidroins that display modular structures. It is known to consist of two proteins: the major ampullate spidroin (MaSp) 1 and MaSp2. This study analyses MaSp sequences from the nursery-web spider Euprosthenops australis. We have identified a previously uncharacterized MaSp2 sequence and a new MaSp-like spidroin, which display distinct homogenous submotifs within their respective Gly-rich repeats. Furthermore, a group of MaSp1 cDNA clones show unexpected heterogeneity. Genomic PCR identified several MaSp1 gene variants within individual spiders, which suggests the presence of a gene cluster in E. australis. Finally, the evolution of spidroin genes is discussed in relation to phylogenetic analysis of nonrepetitive C-terminal domains from diverse species. SN - 0962-1075 UR - https://www.unboundmedicine.com/medline/citation/17680798/Major_ampullate_spidroins_from_Euprosthenops_australis:_multiplicity_at_protein_mRNA_and_gene_levels_ L2 - https://doi.org/10.1111/j.1365-2583.2007.00749.x DB - PRIME DP - Unbound Medicine ER -