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Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation.
Fertil Steril. 2008 Apr; 89(4):912-21.FS

Abstract

OBJECTIVE

To investigate the relationship between oxidative stress and the underlying causes of infertility, preovulatory ovarian hormones, and ovarian response to gonadotropin stimulation in patients undergoing assisted reproductive techniques.

DESIGN

Prospective, cross-sectional study.

SETTING

Assisted conception unit, university hospital.

PATIENT(S)

One hundred thirty women presenting with infertility, of the following types: male factor (n = 56), unexplained (n = 36), tubal factor (n = 16), polycystic ovary syndrome (n = 15), and endometriosis (n = 7).

INTERVENTION(S)

Follicular fluid (FF) and peripheral blood samples were collected at oocyte retrieval.

MAIN OUTCOME MEASURE(S)

Blood and FF samples were analyzed for inhibin A, inhibin B, activin A, anti-Müllerian hormone, and E(2) by using ELISA. Total antioxidant capacity (TAC) was measured in plasma and FF by using a calorimetric microplate assay.

RESULT(S)

There was no significant relationship between plasma or FF TAC and the underlying etiology of infertility. There was a statistically significant positive association between FF E(2) levels and TAC (r = 0.26). Higher antral follicle count, delta E(2) (day 3 E(2) minus day 2 E(2)), preovulatory serum anti-Müllerian hormone, inhibin B, and E(2) were associated with good ovarian response, whereas higher FF E(2) was associated with a statistically significant poor response. No significant direct relationship was observed between TAC and ovarian response as well as between TAC or any of the parameters measured and pregnancy outcome.

CONCLUSION(S)

Oxidative stress has an impact on the production of granulosa cell steroid hormones, in particular E(2), which is an important predictor of ovarian response. The positive association between FF E(2) and total antioxidant capacity suggests that E(2) may play a role in the ovarian antioxidant-oxidant balance.

Authors+Show Affiliations

Academic Department of Obstetrics and Gynaecology, Royal Free and University College London, University College London Campus, London, United Kingdom. apmala01@yahoo.co.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17681319

Citation

Appasamy, Malathy, et al. "Evaluation of the Relationship Between Follicular Fluid Oxidative Stress, Ovarian Hormones, and Response to Gonadotropin Stimulation." Fertility and Sterility, vol. 89, no. 4, 2008, pp. 912-21.
Appasamy M, Jauniaux E, Serhal P, et al. Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. Fertil Steril. 2008;89(4):912-21.
Appasamy, M., Jauniaux, E., Serhal, P., Al-Qahtani, A., Groome, N. P., & Muttukrishna, S. (2008). Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. Fertility and Sterility, 89(4), 912-21.
Appasamy M, et al. Evaluation of the Relationship Between Follicular Fluid Oxidative Stress, Ovarian Hormones, and Response to Gonadotropin Stimulation. Fertil Steril. 2008;89(4):912-21. PubMed PMID: 17681319.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the relationship between follicular fluid oxidative stress, ovarian hormones, and response to gonadotropin stimulation. AU - Appasamy,Malathy, AU - Jauniaux,Eric, AU - Serhal,Paul, AU - Al-Qahtani,Ahmed, AU - Groome,Nigel P, AU - Muttukrishna,Shanthi, Y1 - 2007/08/06/ PY - 2006/10/16/received PY - 2007/04/02/revised PY - 2007/04/02/accepted PY - 2007/8/8/pubmed PY - 2008/4/30/medline PY - 2007/8/8/entrez SP - 912 EP - 21 JF - Fertility and sterility JO - Fertil Steril VL - 89 IS - 4 N2 - OBJECTIVE: To investigate the relationship between oxidative stress and the underlying causes of infertility, preovulatory ovarian hormones, and ovarian response to gonadotropin stimulation in patients undergoing assisted reproductive techniques. DESIGN: Prospective, cross-sectional study. SETTING: Assisted conception unit, university hospital. PATIENT(S): One hundred thirty women presenting with infertility, of the following types: male factor (n = 56), unexplained (n = 36), tubal factor (n = 16), polycystic ovary syndrome (n = 15), and endometriosis (n = 7). INTERVENTION(S): Follicular fluid (FF) and peripheral blood samples were collected at oocyte retrieval. MAIN OUTCOME MEASURE(S): Blood and FF samples were analyzed for inhibin A, inhibin B, activin A, anti-Müllerian hormone, and E(2) by using ELISA. Total antioxidant capacity (TAC) was measured in plasma and FF by using a calorimetric microplate assay. RESULT(S): There was no significant relationship between plasma or FF TAC and the underlying etiology of infertility. There was a statistically significant positive association between FF E(2) levels and TAC (r = 0.26). Higher antral follicle count, delta E(2) (day 3 E(2) minus day 2 E(2)), preovulatory serum anti-Müllerian hormone, inhibin B, and E(2) were associated with good ovarian response, whereas higher FF E(2) was associated with a statistically significant poor response. No significant direct relationship was observed between TAC and ovarian response as well as between TAC or any of the parameters measured and pregnancy outcome. CONCLUSION(S): Oxidative stress has an impact on the production of granulosa cell steroid hormones, in particular E(2), which is an important predictor of ovarian response. The positive association between FF E(2) and total antioxidant capacity suggests that E(2) may play a role in the ovarian antioxidant-oxidant balance. SN - 1556-5653 UR - https://www.unboundmedicine.com/medline/citation/17681319/Evaluation_of_the_relationship_between_follicular_fluid_oxidative_stress_ovarian_hormones_and_response_to_gonadotropin_stimulation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0015-0282(07)00995-8 DB - PRIME DP - Unbound Medicine ER -