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Direct electron transfer and bioelectrocatalysis of hemoglobin on nano-structural attapulgite clay-modified glassy carbon electrode.
J Colloid Interface Sci. 2007 Nov 01; 315(1):170-6.JC

Abstract

Direct electrochemistry of hemoglobin (Hb) on natural nano-structural attapulgite clay film-modified glassy carbon (GC) electrode was investigated. The interaction between Hb and attapulgite was examined using UV-vis, FTIR spectroscopy, and electrochemical methods. The immobilized Hb displayed a couple of well-defined and quasi-reversible redox peaks with the formal potential (E(0')) of about -0.366 V (versus SCE) in 0.1 M phosphate buffer solution of pH 7.0. The current was linearly dependent on the scan rate, indicating that the direct electrochemistry of Hb in that case was a surface-controlled electrode process. The formal potential changed linearly from pH 5.0 to 9.0 with a slope value of -48.2 mV/pH, which suggested that a proton transfer was accompanied with each electron transfer in the electrochemical reaction. The immobilized Hb exhibited excellent electrocatalytic activity for the reduction of hydrogen peroxide without the aid of an electron mediator. The electrocatalytic response showed a linear dependence on the H(2)O(2) concentration ranging from 5.4 x 10(-6) to 4.0 x 10(-4) M with the detection of 2.4 x 10(-6) M at a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant K(M)(app) for the H(2)O(2) sensor was estimated to be 490 microM, showing a high affinity.

Authors+Show Affiliations

Jiangsu Key Laboratory for Chemistry of Low-Dimensional Materials, Huaiyin Teachers College, Jiangsu 223300, People's Republic of China. zujm68@sohu.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17681509

Citation

Xu, Jiming, et al. "Direct Electron Transfer and Bioelectrocatalysis of Hemoglobin On Nano-structural Attapulgite Clay-modified Glassy Carbon Electrode." Journal of Colloid and Interface Science, vol. 315, no. 1, 2007, pp. 170-6.
Xu J, Li W, Yin Q, et al. Direct electron transfer and bioelectrocatalysis of hemoglobin on nano-structural attapulgite clay-modified glassy carbon electrode. J Colloid Interface Sci. 2007;315(1):170-6.
Xu, J., Li, W., Yin, Q., Zhong, H., Zhu, Y., & Jin, L. (2007). Direct electron transfer and bioelectrocatalysis of hemoglobin on nano-structural attapulgite clay-modified glassy carbon electrode. Journal of Colloid and Interface Science, 315(1), 170-6.
Xu J, et al. Direct Electron Transfer and Bioelectrocatalysis of Hemoglobin On Nano-structural Attapulgite Clay-modified Glassy Carbon Electrode. J Colloid Interface Sci. 2007 Nov 1;315(1):170-6. PubMed PMID: 17681509.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electron transfer and bioelectrocatalysis of hemoglobin on nano-structural attapulgite clay-modified glassy carbon electrode. AU - Xu,Jiming, AU - Li,Wei, AU - Yin,Qifan, AU - Zhong,Hui, AU - Zhu,Yulan, AU - Jin,Litong, Y1 - 2007/08/03/ PY - 2007/05/08/received PY - 2007/06/25/revised PY - 2007/06/26/accepted PY - 2007/8/8/pubmed PY - 2008/2/21/medline PY - 2007/8/8/entrez SP - 170 EP - 6 JF - Journal of colloid and interface science JO - J Colloid Interface Sci VL - 315 IS - 1 N2 - Direct electrochemistry of hemoglobin (Hb) on natural nano-structural attapulgite clay film-modified glassy carbon (GC) electrode was investigated. The interaction between Hb and attapulgite was examined using UV-vis, FTIR spectroscopy, and electrochemical methods. The immobilized Hb displayed a couple of well-defined and quasi-reversible redox peaks with the formal potential (E(0')) of about -0.366 V (versus SCE) in 0.1 M phosphate buffer solution of pH 7.0. The current was linearly dependent on the scan rate, indicating that the direct electrochemistry of Hb in that case was a surface-controlled electrode process. The formal potential changed linearly from pH 5.0 to 9.0 with a slope value of -48.2 mV/pH, which suggested that a proton transfer was accompanied with each electron transfer in the electrochemical reaction. The immobilized Hb exhibited excellent electrocatalytic activity for the reduction of hydrogen peroxide without the aid of an electron mediator. The electrocatalytic response showed a linear dependence on the H(2)O(2) concentration ranging from 5.4 x 10(-6) to 4.0 x 10(-4) M with the detection of 2.4 x 10(-6) M at a signal-to-noise ratio of 3. The apparent Michaelis-Menten constant K(M)(app) for the H(2)O(2) sensor was estimated to be 490 microM, showing a high affinity. SN - 0021-9797 UR - https://www.unboundmedicine.com/medline/citation/17681509/Direct_electron_transfer_and_bioelectrocatalysis_of_hemoglobin_on_nano_structural_attapulgite_clay_modified_glassy_carbon_electrode_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9797(07)00937-X DB - PRIME DP - Unbound Medicine ER -