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[Arbitrarily primed-PCR detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children with high dmft and no caries].
Zhonghua Kou Qiang Yi Xue Za Zhi. 2007 Apr; 42(4):219-22.ZK

Abstract

OBJECTIVE

To isolate and identify Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss) in dental plaque of children with high dmft and no caries by selective medium, biochemical methods and arbitrarily primed-polymerase chain reaction (AP-PCR).

METHODS

A total of 401 3-4-year-old children from seven kindergartens were recruited using cluster sampling and their dental caries status were examined. From 30% of children with the highest dmft score (dmft >/= 5), 20 children were chosen randomly as test group and 20 age and gender-matched caries-free children were selected as control. Plaque samples were collected from buccal surfaces of the molars and plated onto TYCSB plate. Sm and Ss were primarily identified by colony morphology and biochemical characteristics. Then chromosomal DNA of the strains was isolated and Sm or Ss were confirmed by AP-PCR.

RESULTS

The proportion positive for Sm and Ss in children with high dmft was 100% and 40% respectively while that in caries-free children was 75% and 5% by AP-PCR analysis. The differences were statistically significant between the two groups.

CONCLUSIONS

The proportions positive for Sm and Ss detected by AP-PCR method were significantly higher in children with high dmft than in caries-free children and it is a risk factor for high dmft in deciduous teeth harboring Sm and Ss.

Authors+Show Affiliations

Department of Dental Public Health, Guanghua School of Stomatology, Sun Yat-sen University, Guangzhou 510055, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

chi

PubMed ID

17683669

Citation

Zhi, Qing-hui, et al. "[Arbitrarily primed-PCR Detection of Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque of Children With High Dmft and No Caries]." Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, vol. 42, no. 4, 2007, pp. 219-22.
Zhi QH, Lin HC, Zhang R, et al. [Arbitrarily primed-PCR detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children with high dmft and no caries]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2007;42(4):219-22.
Zhi, Q. H., Lin, H. C., Zhang, R., Liao, Y. D., & Tu, J. Z. (2007). [Arbitrarily primed-PCR detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children with high dmft and no caries]. Zhonghua Kou Qiang Yi Xue Za Zhi = Zhonghua Kouqiang Yixue Zazhi = Chinese Journal of Stomatology, 42(4), 219-22.
Zhi QH, et al. [Arbitrarily primed-PCR Detection of Streptococcus Mutans and Streptococcus Sobrinus in Dental Plaque of Children With High Dmft and No Caries]. Zhonghua Kou Qiang Yi Xue Za Zhi. 2007;42(4):219-22. PubMed PMID: 17683669.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Arbitrarily primed-PCR detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children with high dmft and no caries]. AU - Zhi,Qing-hui, AU - Lin,Huan-cai, AU - Zhang,Rong, AU - Liao,Yi-dong, AU - Tu,Jia-zhen, PY - 2007/8/9/pubmed PY - 2010/6/30/medline PY - 2007/8/9/entrez SP - 219 EP - 22 JF - Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology JO - Zhonghua Kou Qiang Yi Xue Za Zhi VL - 42 IS - 4 N2 - OBJECTIVE: To isolate and identify Streptococcus mutans (Sm) and Streptococcus sobrinus (Ss) in dental plaque of children with high dmft and no caries by selective medium, biochemical methods and arbitrarily primed-polymerase chain reaction (AP-PCR). METHODS: A total of 401 3-4-year-old children from seven kindergartens were recruited using cluster sampling and their dental caries status were examined. From 30% of children with the highest dmft score (dmft >/= 5), 20 children were chosen randomly as test group and 20 age and gender-matched caries-free children were selected as control. Plaque samples were collected from buccal surfaces of the molars and plated onto TYCSB plate. Sm and Ss were primarily identified by colony morphology and biochemical characteristics. Then chromosomal DNA of the strains was isolated and Sm or Ss were confirmed by AP-PCR. RESULTS: The proportion positive for Sm and Ss in children with high dmft was 100% and 40% respectively while that in caries-free children was 75% and 5% by AP-PCR analysis. The differences were statistically significant between the two groups. CONCLUSIONS: The proportions positive for Sm and Ss detected by AP-PCR method were significantly higher in children with high dmft than in caries-free children and it is a risk factor for high dmft in deciduous teeth harboring Sm and Ss. SN - 1002-0098 UR - https://www.unboundmedicine.com/medline/citation/17683669/[Arbitrarily_primed_PCR_detection_of_Streptococcus_mutans_and_Streptococcus_sobrinus_in_dental_plaque_of_children_with_high_dmft_and_no_caries]_ L2 - http://journal.yiigle.com/LinkIn.do?linkin_type=pubmed&issn=1002-0098&year=2007&vol=42&issue=4&fpage=219 DB - PRIME DP - Unbound Medicine ER -