TY - JOUR T1 - Protective effects of Hibiscus tiliaceus L. methanolic extract to V79 cells against cytotoxicity and genotoxicity induced by hydrogen peroxide and tert-butyl-hydroperoxide. AU - Rosa,Renato Moreira, AU - Moura,Dinara Jaqueline, AU - Melecchi,Maria Inês S, AU - dos Santos,Rafael Santos, AU - Richter,Marc François, AU - Camarão,Elina Bastos, AU - Henriques,João Antonio Pêgas, AU - de Paula Ramos,Ana Lígia Lia, AU - Saffi,Jenifer, Y1 - 2007/06/27/ PY - 2007/05/15/received PY - 2007/06/06/revised PY - 2007/06/07/accepted PY - 2007/8/9/pubmed PY - 2008/2/19/medline PY - 2007/8/9/entrez SP - 1442 EP - 52 JF - Toxicology in vitro : an international journal published in association with BIBRA JO - Toxicol In Vitro VL - 21 IS - 8 N2 - Plants of the genus Hibiscus thrives produce a diversity of molecules with bioactive properties. In a previous study of Hibiscus tiliaceus L. methanolic extract (HME) using bacteria and yeast, as test media, it has been shown that HME strongly inhibited the mutagenic action of H(2)O(2) or tert-butyl-hydroperoxide (t-BHP). Here, our interest is to evaluate the genotoxicity and the antigenotoxic/antimutagenic properties of HME using oxidative challenge with H(2)O(2) and t-BHP in V79 cells. We determined cytotoxicity using clonal survival assay; evaluated DNA damage using the comet assay and the micronucleus test in binucleated cells besides of the lipid peroxidation degree and the reduced glutathione content. We examined the ability of HME in quenching hydroxyl radical by means of a HPLC-based method utilizing the hypoxanthine/xanthine oxidase assay. At concentrations ranging from 0.001 to 0.1mg/mL, HME was not cytotoxic, genotoxic or mutagenic. Treatment with non-cytotoxic concentrations of HME increased cell survival after H(2)O(2) and t-BHP exposure and prevented DNA damage. The pre-treatment with HME also was able to decrease the mutagenic effect of these genotoxins, evaluated using the micronucleus test. HME prevented the increase in lipid peroxidation and decrease in GSH content in response to the oxidative challenge. Therefore, the ability in preventing against H(2)O(2)- and t-BHP-induced GSH depletion and lipid peroxidation was probably a major contribution to the cytoprotective effects. Moreover, HME acts as a hydroxyl radical scavenger. In summary, HME did not have a harmful or inhibitory effect on the growth of V79 cells and presented antioxidant activity, consequently, both antigenotoxic and antimutagenic effects against oxidative DNA damage. SN - 0887-2333 UR - https://www.unboundmedicine.com/medline/citation/17683899/Protective_effects_of_Hibiscus_tiliaceus_L__methanolic_extract_to_V79_cells_against_cytotoxicity_and_genotoxicity_induced_by_hydrogen_peroxide_and_tert_butyl_hydroperoxide_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0887-2333(07)00176-2 DB - PRIME DP - Unbound Medicine ER -