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Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained Thick Film (GTF) for diagnosis of malaria.
Parasitol Int. 2007 Dec; 56(4):308-12.PI

Abstract

The renewed interest in the use of fluorescent microscopy for malaria diagnosis prompted the comparison of Quantitative Buffy Coat technique (QBC) with the old standard Giemsa-stained Thick blood Film (GTF) in Ikeja General Hospital, now Lagos State University Teaching Hospital, in Lagos. Blood samples were collected from 353 patients, each examined with the QBC and GTF techniques. Of these, 68 were positive with GTF, 70 with QBC giving a positive rate of 19.3% and 19.8% respectively. The malaria positive rate was calculated as 19.3% using GTF as the standard. In general, females recorded higher percentages (58.6% and 54.4%) than males (41.4% and 45.6%) among those positive with QBC and GTF respectively. The overall sensitivity rate for QBC was 55.9% and the specificity was 88.8%. The positive and negative predictive values of QBC compared to GTF were 54.3%, 89.4% respectively while the concordance of the two techniques was 82.4%. These values were lower than those reported for QBC in previous studies. The sensitivity of QBC reduced further (33.3%) with samples having low parasite density (<1000 parasites/ul). QBC test was not able to accurately differentiate between different Plasmodium species but with the GTF, 86.7% of the infected individuals had Plasmodium falciparum, 7.5% had P. malariae and only 5.9% had mixed infections. In spite of the speed and simplicity of QBC technique, it cannot be considered an acceptable alternative to GTF under routine clinical laboratory situation. However, its speed and ease of use make it an important new tool for the diagnosis of malaria.

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Authors+Show Affiliations

Adeoye GO
Department of Zoology, University of Lagos, Akoka, Yaba, Lagos, Nigeria. goa4567@yahoo.com
Nga IC
No affiliation info available

MeSH

AnimalsAzure StainsColoring AgentsFemaleHematologic TestsHumansMalariaMalaria, FalciparumMaleParasitemiaPlasmodium falciparumPlasmodium malariaePredictive Value of TestsSensitivity and Specificity

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article

Language

eng

PubMed ID

17683979

Citation

Adeoye, G O., and I C. Nga. "Comparison of Quantitative Buffy Coat Technique (QBC) With Giemsa-stained Thick Film (GTF) for Diagnosis of Malaria." Parasitology International, vol. 56, no. 4, 2007, pp. 308-12.
Adeoye GO, Nga IC. Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained Thick Film (GTF) for diagnosis of malaria. Parasitol Int. 2007;56(4):308-12.
Adeoye, G. O., & Nga, I. C. (2007). Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained Thick Film (GTF) for diagnosis of malaria. Parasitology International, 56(4), 308-12.
Adeoye GO, Nga IC. Comparison of Quantitative Buffy Coat Technique (QBC) With Giemsa-stained Thick Film (GTF) for Diagnosis of Malaria. Parasitol Int. 2007;56(4):308-12. PubMed PMID: 17683979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of Quantitative Buffy Coat technique (QBC) with Giemsa-stained Thick Film (GTF) for diagnosis of malaria. AU - Adeoye,G O, AU - Nga,I C, Y1 - 2007/06/30/ PY - 2007/04/30/received PY - 2007/06/19/revised PY - 2007/06/20/accepted PY - 2007/8/9/pubmed PY - 2007/12/6/medline PY - 2007/8/9/entrez SP - 308 EP - 12 JF - Parasitology international JO - Parasitol Int VL - 56 IS - 4 N2 - The renewed interest in the use of fluorescent microscopy for malaria diagnosis prompted the comparison of Quantitative Buffy Coat technique (QBC) with the old standard Giemsa-stained Thick blood Film (GTF) in Ikeja General Hospital, now Lagos State University Teaching Hospital, in Lagos. Blood samples were collected from 353 patients, each examined with the QBC and GTF techniques. Of these, 68 were positive with GTF, 70 with QBC giving a positive rate of 19.3% and 19.8% respectively. The malaria positive rate was calculated as 19.3% using GTF as the standard. In general, females recorded higher percentages (58.6% and 54.4%) than males (41.4% and 45.6%) among those positive with QBC and GTF respectively. The overall sensitivity rate for QBC was 55.9% and the specificity was 88.8%. The positive and negative predictive values of QBC compared to GTF were 54.3%, 89.4% respectively while the concordance of the two techniques was 82.4%. These values were lower than those reported for QBC in previous studies. The sensitivity of QBC reduced further (33.3%) with samples having low parasite density (<1000 parasites/ul). QBC test was not able to accurately differentiate between different Plasmodium species but with the GTF, 86.7% of the infected individuals had Plasmodium falciparum, 7.5% had P. malariae and only 5.9% had mixed infections. In spite of the speed and simplicity of QBC technique, it cannot be considered an acceptable alternative to GTF under routine clinical laboratory situation. However, its speed and ease of use make it an important new tool for the diagnosis of malaria. SN - 1383-5769 UR - https://www.unboundmedicine.com/medline/citation/17683979/Comparison_of_Quantitative_Buffy_Coat_technique__QBC__with_Giemsa_stained_Thick_Film__GTF__for_diagnosis_of_malaria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5769(07)00094-3 DB - PRIME DP - Unbound Medicine ER -