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Vanilloid receptor 1 (TRPV1/VR1) co-localizes with fatty acid amide hydrolase (FAAH) in retinal amacrine cells.
Vis Neurosci 2007 Jul-Aug; 24(4):581-91VN

Abstract

Fatty acid amide hydrolase (FAAH) is the degradative enzyme for anandamide (AEA), an endogenous ligand for the vanilloid receptor (TRPV1) and cannabinoid receptor 1. As FAAH and TRPV1 are integral membrane proteins, FAAH activity could modulate the availability of AEA for TRPV1 activation. Previous studies in this laboratory reported an extensive endocannabinoid system in goldfish retina. Immunocytochemistry was used to determine the relative distributions of FAAH-immunoreactivity (IR) and TRPV1-IR in goldfish retina. Here, we show the first example in an intact neural system in which TRPV1-IR co-localizes in subpopulations of FAAH-immunoreactive neurons, in this case amacrine cells. These cells are rare and consist of three subtypes: 1. ovoid cell with granular-type dendrites restricted to sublamina a, 2. pyriform cell with smooth processes in sublamina b, and 3. fusiform cell with smooth processes that project to sublaminae a and b. The varied appearances of reaction product in the dendrites suggest different subcellular localization of TRPV1, and hence function of FAAH activity regarding TRPV1 stimulation among the cell types. Ovoid and pyriform amacrine cells, but not fusiform cells, labeled with GAD-IR and constituted subsets of GABAergic amacrine cells. TRPV1 amacrine cells, though rare, are represented in the ON, OFF and ON/OFF pathways of the retina. As TRPV1 stimulation increases intracellular calcium with numerous downstream effects, co-localization of TRPV1 and FAAH suggests an autoregulatory function for anandamide. Due to the rarity of these cells, the three vanilloid amacrine cell types may be involved in global effects rather than feature extraction, for example: sampling of ambient light or maintaining homeostasis.

Authors+Show Affiliations

Graduate Program in Neuroscience, Stony Brook University, Stony Brook, New York 11794, USA.

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

17686199

Citation

Zimov, Sarah, and Stephen Yazulla. "Vanilloid Receptor 1 (TRPV1/VR1) Co-localizes With Fatty Acid Amide Hydrolase (FAAH) in Retinal Amacrine Cells." Visual Neuroscience, vol. 24, no. 4, 2007, pp. 581-91.
Zimov S, Yazulla S. Vanilloid receptor 1 (TRPV1/VR1) co-localizes with fatty acid amide hydrolase (FAAH) in retinal amacrine cells. Vis Neurosci. 2007;24(4):581-91.
Zimov, S., & Yazulla, S. (2007). Vanilloid receptor 1 (TRPV1/VR1) co-localizes with fatty acid amide hydrolase (FAAH) in retinal amacrine cells. Visual Neuroscience, 24(4), pp. 581-91.
Zimov S, Yazulla S. Vanilloid Receptor 1 (TRPV1/VR1) Co-localizes With Fatty Acid Amide Hydrolase (FAAH) in Retinal Amacrine Cells. Vis Neurosci. 2007;24(4):581-91. PubMed PMID: 17686199.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vanilloid receptor 1 (TRPV1/VR1) co-localizes with fatty acid amide hydrolase (FAAH) in retinal amacrine cells. AU - Zimov,Sarah, AU - Yazulla,Stephen, Y1 - 2007/08/09/ PY - 2007/02/02/received PY - 2007/05/23/accepted PY - 2007/8/10/pubmed PY - 2007/12/6/medline PY - 2007/8/10/entrez SP - 581 EP - 91 JF - Visual neuroscience JO - Vis. Neurosci. VL - 24 IS - 4 N2 - Fatty acid amide hydrolase (FAAH) is the degradative enzyme for anandamide (AEA), an endogenous ligand for the vanilloid receptor (TRPV1) and cannabinoid receptor 1. As FAAH and TRPV1 are integral membrane proteins, FAAH activity could modulate the availability of AEA for TRPV1 activation. Previous studies in this laboratory reported an extensive endocannabinoid system in goldfish retina. Immunocytochemistry was used to determine the relative distributions of FAAH-immunoreactivity (IR) and TRPV1-IR in goldfish retina. Here, we show the first example in an intact neural system in which TRPV1-IR co-localizes in subpopulations of FAAH-immunoreactive neurons, in this case amacrine cells. These cells are rare and consist of three subtypes: 1. ovoid cell with granular-type dendrites restricted to sublamina a, 2. pyriform cell with smooth processes in sublamina b, and 3. fusiform cell with smooth processes that project to sublaminae a and b. The varied appearances of reaction product in the dendrites suggest different subcellular localization of TRPV1, and hence function of FAAH activity regarding TRPV1 stimulation among the cell types. Ovoid and pyriform amacrine cells, but not fusiform cells, labeled with GAD-IR and constituted subsets of GABAergic amacrine cells. TRPV1 amacrine cells, though rare, are represented in the ON, OFF and ON/OFF pathways of the retina. As TRPV1 stimulation increases intracellular calcium with numerous downstream effects, co-localization of TRPV1 and FAAH suggests an autoregulatory function for anandamide. Due to the rarity of these cells, the three vanilloid amacrine cell types may be involved in global effects rather than feature extraction, for example: sampling of ambient light or maintaining homeostasis. SN - 0952-5238 UR - https://www.unboundmedicine.com/medline/citation/17686199/Vanilloid_receptor_1__TRPV1/VR1__co_localizes_with_fatty_acid_amide_hydrolase__FAAH__in_retinal_amacrine_cells_ L2 - https://www.cambridge.org/core/product/identifier/S095252380707054X/type/journal_article DB - PRIME DP - Unbound Medicine ER -