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Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice.
Vaccine 2007; 25(37-38):6721-9V

Abstract

The immunogenicity and protective efficacy of recombinant SurA (rSurA) and rDnaK from Brucella spp. were evaluated in BALB/c mice. Immunization with rSurA in adjuvant induced a vigorous immunoglobulin G (IgG) response, with higher IgG2a than IgG1 titers. In addition, after in vitro stimulation with rSurA, spleen cells from rSurA-immunized mice produced interleukin-2 (IL-2), interferon (IFN)-gamma, IL-4 and IL-5. Immunization with rDnaK plus adjuvant induced a strong humoral response resulting in similar anti-rDnaK IgG titers than immunization with rDnaK alone. IgG2a titers predominated over IgG1 in mice injected with rDnaK alone or rDnaK plus adjuvant. Spleen cells from mice immunized with rDnaK plus adjuvant secreted IFN-gamma and IL-2 upon stimulation with rDnaK and induced a specific cytotoxic response. On the contrary, mice immunized with rDnaK alone did not exhibit a specific T helper or cytotoxic response in vitro. Mice given rSurA or rDnaK with adjuvant exhibited a significant degree of protection whereas immunization with rDnaK alone induced a low but still statistically significant level of protection against B. abortus infection. All studied vaccines were less protected than mice immunized with H38 or B. abortus strain 19 control vaccines. Altogether these results suggest that rSurA or rDnaK induce partial protection against B. abortus infection and could be useful candidates for the development of subunit vaccines against brucellosis.

Authors+Show Affiliations

Instituto de Estudios de la Inmunidad Humoral (IDEHU-CONICET), Facultad de Farmacia y Bioquímica, UBA, Buenos Aires, Argentina.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17686554

Citation

Delpino, Maria Victoria, et al. "Vaccination With Brucella Recombinant DnaK and SurA Proteins Induces Protection Against Brucella Abortus Infection in BALB/c Mice." Vaccine, vol. 25, no. 37-38, 2007, pp. 6721-9.
Delpino MV, Estein SM, Fossati CA, et al. Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice. Vaccine. 2007;25(37-38):6721-9.
Delpino, M. V., Estein, S. M., Fossati, C. A., Baldi, P. C., & Cassataro, J. (2007). Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice. Vaccine, 25(37-38), pp. 6721-9.
Delpino MV, et al. Vaccination With Brucella Recombinant DnaK and SurA Proteins Induces Protection Against Brucella Abortus Infection in BALB/c Mice. Vaccine. 2007 Sep 17;25(37-38):6721-9. PubMed PMID: 17686554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vaccination with Brucella recombinant DnaK and SurA proteins induces protection against Brucella abortus infection in BALB/c mice. AU - Delpino,Maria Victoria, AU - Estein,Silvia Marcela, AU - Fossati,Carlos Alberto, AU - Baldi,Pablo César, AU - Cassataro,Juliana, Y1 - 2007/07/23/ PY - 2007/05/16/received PY - 2007/06/28/revised PY - 2007/07/04/accepted PY - 2007/8/10/pubmed PY - 2007/11/9/medline PY - 2007/8/10/entrez SP - 6721 EP - 9 JF - Vaccine JO - Vaccine VL - 25 IS - 37-38 N2 - The immunogenicity and protective efficacy of recombinant SurA (rSurA) and rDnaK from Brucella spp. were evaluated in BALB/c mice. Immunization with rSurA in adjuvant induced a vigorous immunoglobulin G (IgG) response, with higher IgG2a than IgG1 titers. In addition, after in vitro stimulation with rSurA, spleen cells from rSurA-immunized mice produced interleukin-2 (IL-2), interferon (IFN)-gamma, IL-4 and IL-5. Immunization with rDnaK plus adjuvant induced a strong humoral response resulting in similar anti-rDnaK IgG titers than immunization with rDnaK alone. IgG2a titers predominated over IgG1 in mice injected with rDnaK alone or rDnaK plus adjuvant. Spleen cells from mice immunized with rDnaK plus adjuvant secreted IFN-gamma and IL-2 upon stimulation with rDnaK and induced a specific cytotoxic response. On the contrary, mice immunized with rDnaK alone did not exhibit a specific T helper or cytotoxic response in vitro. Mice given rSurA or rDnaK with adjuvant exhibited a significant degree of protection whereas immunization with rDnaK alone induced a low but still statistically significant level of protection against B. abortus infection. All studied vaccines were less protected than mice immunized with H38 or B. abortus strain 19 control vaccines. Altogether these results suggest that rSurA or rDnaK induce partial protection against B. abortus infection and could be useful candidates for the development of subunit vaccines against brucellosis. SN - 0264-410X UR - https://www.unboundmedicine.com/medline/citation/17686554/Vaccination_with_Brucella_recombinant_DnaK_and_SurA_proteins_induces_protection_against_Brucella_abortus_infection_in_BALB/c_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(07)00778-5 DB - PRIME DP - Unbound Medicine ER -