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Identification of dermatophytes by an oligonucleotide array.
J Clin Microbiol. 2007 Oct; 45(10):3160-6.JC

Abstract

Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify dermatophytes are often lengthy and may be inconclusive because of atypical microscopic or colony morphology. Based on the internal transcribed spacer 1 (ITS-1) and ITS-2 sequences of the rRNA genes, an oligonucleotide array was developed to identify 17 dermatophyte species. The method consisted of PCR amplification of the ITS regions using universal primers, followed by hybridization of the digoxigenin-labeled PCR products to an array of oligonucleotides (17- to 30-mers) immobilized on a nylon membrane. Of 198 dermatophyte strains and 90 nontarget strains tested, the sensitivity and specificity of the array were 99.5% and 97.8%, respectively. The only strain not identified (Microsporum audouinii LMA 597) was found to have a nucleotide insertion at the ITS-2 region where the probe was designed. Two nontarget strains, Microsporum equinum LMA 40396666 and Trichophyton gourvilii var. intermedium CBS 170.65, were misidentified as Microsporum canis and Trichophyton soudanense, respectively. Sequence analysis of the ITS regions revealed that the two misidentified strains displayed high sequence homology with the probes designed for M. canis and T. soudanense, respectively. The present method can be used as a reliable alternative to conventional identification methods and can be completed with isolated colonies within 24 h.

Authors+Show Affiliations

Department of Medical Laboratory Science and Biotechnology, School of Medicine, National Cheng Kung University, 1 University Road, Tainan 701, Taiwan, Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17687010

Citation

Li, Hsin Chieh, et al. "Identification of Dermatophytes By an Oligonucleotide Array." Journal of Clinical Microbiology, vol. 45, no. 10, 2007, pp. 3160-6.
Li HC, Bouchara JP, Hsu MM, et al. Identification of dermatophytes by an oligonucleotide array. J Clin Microbiol. 2007;45(10):3160-6.
Li, H. C., Bouchara, J. P., Hsu, M. M., Barton, R., & Chang, T. C. (2007). Identification of dermatophytes by an oligonucleotide array. Journal of Clinical Microbiology, 45(10), 3160-6.
Li HC, et al. Identification of Dermatophytes By an Oligonucleotide Array. J Clin Microbiol. 2007;45(10):3160-6. PubMed PMID: 17687010.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of dermatophytes by an oligonucleotide array. AU - Li,Hsin Chieh, AU - Bouchara,Jean-Philippe, AU - Hsu,Mark Ming-Long, AU - Barton,Richard, AU - Chang,Tsung Chain, Y1 - 2007/08/08/ PY - 2007/8/10/pubmed PY - 2007/12/6/medline PY - 2007/8/10/entrez SP - 3160 EP - 6 JF - Journal of clinical microbiology JO - J. Clin. Microbiol. VL - 45 IS - 10 N2 - Species of dermatophytes are classified into three anamorphic (asexual) genera, Epidermophyton, Microsporum, and Trichophyton. Conventional methods used to identify dermatophytes are often lengthy and may be inconclusive because of atypical microscopic or colony morphology. Based on the internal transcribed spacer 1 (ITS-1) and ITS-2 sequences of the rRNA genes, an oligonucleotide array was developed to identify 17 dermatophyte species. The method consisted of PCR amplification of the ITS regions using universal primers, followed by hybridization of the digoxigenin-labeled PCR products to an array of oligonucleotides (17- to 30-mers) immobilized on a nylon membrane. Of 198 dermatophyte strains and 90 nontarget strains tested, the sensitivity and specificity of the array were 99.5% and 97.8%, respectively. The only strain not identified (Microsporum audouinii LMA 597) was found to have a nucleotide insertion at the ITS-2 region where the probe was designed. Two nontarget strains, Microsporum equinum LMA 40396666 and Trichophyton gourvilii var. intermedium CBS 170.65, were misidentified as Microsporum canis and Trichophyton soudanense, respectively. Sequence analysis of the ITS regions revealed that the two misidentified strains displayed high sequence homology with the probes designed for M. canis and T. soudanense, respectively. The present method can be used as a reliable alternative to conventional identification methods and can be completed with isolated colonies within 24 h. SN - 0095-1137 UR - https://www.unboundmedicine.com/medline/citation/17687010/Identification_of_dermatophytes_by_an_oligonucleotide_array_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=17687010 DB - PRIME DP - Unbound Medicine ER -