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Association of apolipoprotein E gene polymorphism with ischemic stroke involving large-vessel disease and its relation to serum lipid levels.
J Stroke Cerebrovasc Dis 2007 Jul-Aug; 16(4):160-6JS

Abstract

A relationship between apolipoprotein E (Apo E) genotype and stroke was previously suggested, but with inconsistent results. We investigated the relationships among serum lipid levels, Apo E alleles and genotypes, and stroke risk factors in 216 stroke patients and 282 age- and sex-matched controls. Fasting blood samples were collected for total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride level determination and for genomic DNA extraction. Apo was genotyped by polymerase chain reaction-restriction fragment length polymorphism (Cfo I) analysis. Increasing levels of total cholesterol, LDL-C, HDL-C, and triglycerides were associated with elevated stroke risk and was more pronounced in Apo E4-carrying subjects than in E3- and/or E2-carrying subjects. Apo 3 was significantly lower (0.546 vs 0.736; P < .001), whereas Apo 4 was higher in the stroke patients (0.370 vs 0.181; P < .001); Apo 2 was present at low but comparable frequencies. The prevalence of E3/E3 was lower and that of E4-containing phenotypes (E3/E4 and homozygous E4/E4) was higher in the stroke patients. The prevalence of the E4-containing phenotypes were significantly higher in ischemic versus hemorrhagic (P < .001) and in small-vessel versus large-vessel stroke cases (P < .001), and was associated with increased need for statin drugs (P = .040). Logistic regression models, after adjusting for potentially confounding variables including lipid profile, age, and sex, showed an significant association of apo 4 genotype with risk of stroke (P = .033). Our findings indicate that Apo 4 is an independent risk factor associated with an altered lipid profile in this study population.

Authors+Show Affiliations

Research Unit of Hematologic and Autoimmune Diseases, Faculty of Pharmacy, University of Monastir, Monastir, Tunisia.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

17689412

Citation

Saidi, Sarra, et al. "Association of Apolipoprotein E Gene Polymorphism With Ischemic Stroke Involving Large-vessel Disease and Its Relation to Serum Lipid Levels." Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association, vol. 16, no. 4, 2007, pp. 160-6.
Saidi S, Slamia LB, Ammou SB, et al. Association of apolipoprotein E gene polymorphism with ischemic stroke involving large-vessel disease and its relation to serum lipid levels. J Stroke Cerebrovasc Dis. 2007;16(4):160-6.
Saidi, S., Slamia, L. B., Ammou, S. B., Mahjoub, T., & Almawi, W. Y. (2007). Association of apolipoprotein E gene polymorphism with ischemic stroke involving large-vessel disease and its relation to serum lipid levels. Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association, 16(4), pp. 160-6.
Saidi S, et al. Association of Apolipoprotein E Gene Polymorphism With Ischemic Stroke Involving Large-vessel Disease and Its Relation to Serum Lipid Levels. J Stroke Cerebrovasc Dis. 2007;16(4):160-6. PubMed PMID: 17689412.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of apolipoprotein E gene polymorphism with ischemic stroke involving large-vessel disease and its relation to serum lipid levels. AU - Saidi,Sarra, AU - Slamia,Lamia B, AU - Ammou,Sofyan B, AU - Mahjoub,Touhami, AU - Almawi,Wassim Y, PY - 2007/01/11/received PY - 2007/03/14/revised PY - 2007/03/14/accepted PY - 2007/8/11/pubmed PY - 2007/9/15/medline PY - 2007/8/11/entrez SP - 160 EP - 6 JF - Journal of stroke and cerebrovascular diseases : the official journal of National Stroke Association JO - J Stroke Cerebrovasc Dis VL - 16 IS - 4 N2 - A relationship between apolipoprotein E (Apo E) genotype and stroke was previously suggested, but with inconsistent results. We investigated the relationships among serum lipid levels, Apo E alleles and genotypes, and stroke risk factors in 216 stroke patients and 282 age- and sex-matched controls. Fasting blood samples were collected for total cholesterol, low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglyceride level determination and for genomic DNA extraction. Apo was genotyped by polymerase chain reaction-restriction fragment length polymorphism (Cfo I) analysis. Increasing levels of total cholesterol, LDL-C, HDL-C, and triglycerides were associated with elevated stroke risk and was more pronounced in Apo E4-carrying subjects than in E3- and/or E2-carrying subjects. Apo 3 was significantly lower (0.546 vs 0.736; P < .001), whereas Apo 4 was higher in the stroke patients (0.370 vs 0.181; P < .001); Apo 2 was present at low but comparable frequencies. The prevalence of E3/E3 was lower and that of E4-containing phenotypes (E3/E4 and homozygous E4/E4) was higher in the stroke patients. The prevalence of the E4-containing phenotypes were significantly higher in ischemic versus hemorrhagic (P < .001) and in small-vessel versus large-vessel stroke cases (P < .001), and was associated with increased need for statin drugs (P = .040). Logistic regression models, after adjusting for potentially confounding variables including lipid profile, age, and sex, showed an significant association of apo 4 genotype with risk of stroke (P = .033). Our findings indicate that Apo 4 is an independent risk factor associated with an altered lipid profile in this study population. SN - 1532-8511 UR - https://www.unboundmedicine.com/medline/citation/17689412/Association_of_apolipoprotein_E_gene_polymorphism_with_ischemic_stroke_involving_large_vessel_disease_and_its_relation_to_serum_lipid_levels_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1052-3057(07)00037-7 DB - PRIME DP - Unbound Medicine ER -