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Cysteinyl leukotriene receptor antagonist MK-571 alters bronchoalveolar lavage fluid proteome in a mouse asthma model.
Eur J Pharmacol. 2007 Dec 01; 575(1-3):134-41.EJ

Abstract

Cysteinyl leukotriene receptor type 1 (leukotriene CysLT(1) receptor) antagonist is one of the most effective anti-inflammatory agents for asthma. The spectrum of protein targets that can be regulated by leukotriene CysLT(1) receptor antagonist in asthma is not fully understood. The present study tried to identify novel protein targets of a selective leukotriene CysLT(1) receptor antagonist MK-571 in allergic airway inflammation by analyzing the proteome of mouse bronchoalveolar lavage fluid. BALB/c mice sensitized and challenged with ovalbumin showed increased pulmonary inflammatory cell infiltration, airway mucus production and serum ovalbumin-specific IgE level. MK-571 inhibited all these allergic airway inflammation endpoints. Lavage fluid proteins were resolved by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The level of fourteen bronchoalveolar lavage fluid protein spots was markedly altered by MK-571. A family of chitinases (Ym1, Ym2 and acidic mammalian chitinase), lungkine, surfactant protein-D and gamma-actin have been found for the first time to be down-regulated by leukotriene CysLT(1) receptor antagonist in mouse allergic airways. Some of the down-regulatory effects were confirmed with reverse transcription-polymerase chain reaction analyses. Taken together, we have identified novel protein targets that can be regulated by leukotriene CysLT(1) receptor antagonist in mouse allergic airway inflammation, and our findings reveal additional pharmacological actions of leukotriene CysLT(1) receptor antagonist in the treatment of asthma.

Authors+Show Affiliations

Department of Pharmacology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore. phcwongf@nus.edu.sgNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

17689528

Citation

Wong, W S Fred, et al. "Cysteinyl Leukotriene Receptor Antagonist MK-571 Alters Bronchoalveolar Lavage Fluid Proteome in a Mouse Asthma Model." European Journal of Pharmacology, vol. 575, no. 1-3, 2007, pp. 134-41.
Wong WS, Zhu H, Liao W. Cysteinyl leukotriene receptor antagonist MK-571 alters bronchoalveolar lavage fluid proteome in a mouse asthma model. Eur J Pharmacol. 2007;575(1-3):134-41.
Wong, W. S., Zhu, H., & Liao, W. (2007). Cysteinyl leukotriene receptor antagonist MK-571 alters bronchoalveolar lavage fluid proteome in a mouse asthma model. European Journal of Pharmacology, 575(1-3), 134-41.
Wong WS, Zhu H, Liao W. Cysteinyl Leukotriene Receptor Antagonist MK-571 Alters Bronchoalveolar Lavage Fluid Proteome in a Mouse Asthma Model. Eur J Pharmacol. 2007 Dec 1;575(1-3):134-41. PubMed PMID: 17689528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cysteinyl leukotriene receptor antagonist MK-571 alters bronchoalveolar lavage fluid proteome in a mouse asthma model. AU - Wong,W S Fred, AU - Zhu,Hua, AU - Liao,Wupeng, Y1 - 2007/07/24/ PY - 2007/04/16/received PY - 2007/07/07/revised PY - 2007/07/16/accepted PY - 2007/8/11/pubmed PY - 2008/3/7/medline PY - 2007/8/11/entrez SP - 134 EP - 41 JF - European journal of pharmacology JO - Eur. J. Pharmacol. VL - 575 IS - 1-3 N2 - Cysteinyl leukotriene receptor type 1 (leukotriene CysLT(1) receptor) antagonist is one of the most effective anti-inflammatory agents for asthma. The spectrum of protein targets that can be regulated by leukotriene CysLT(1) receptor antagonist in asthma is not fully understood. The present study tried to identify novel protein targets of a selective leukotriene CysLT(1) receptor antagonist MK-571 in allergic airway inflammation by analyzing the proteome of mouse bronchoalveolar lavage fluid. BALB/c mice sensitized and challenged with ovalbumin showed increased pulmonary inflammatory cell infiltration, airway mucus production and serum ovalbumin-specific IgE level. MK-571 inhibited all these allergic airway inflammation endpoints. Lavage fluid proteins were resolved by two-dimensional gel electrophoresis and identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The level of fourteen bronchoalveolar lavage fluid protein spots was markedly altered by MK-571. A family of chitinases (Ym1, Ym2 and acidic mammalian chitinase), lungkine, surfactant protein-D and gamma-actin have been found for the first time to be down-regulated by leukotriene CysLT(1) receptor antagonist in mouse allergic airways. Some of the down-regulatory effects were confirmed with reverse transcription-polymerase chain reaction analyses. Taken together, we have identified novel protein targets that can be regulated by leukotriene CysLT(1) receptor antagonist in mouse allergic airway inflammation, and our findings reveal additional pharmacological actions of leukotriene CysLT(1) receptor antagonist in the treatment of asthma. SN - 0014-2999 UR - https://www.unboundmedicine.com/medline/citation/17689528/Cysteinyl_leukotriene_receptor_antagonist_MK_571_alters_bronchoalveolar_lavage_fluid_proteome_in_a_mouse_asthma_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(07)00799-6 DB - PRIME DP - Unbound Medicine ER -