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Differential expression of gill Na+,K+-ATPase alpha- and beta-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar.
J Exp Biol 2007; 210(Pt 16):2885-96JE

Abstract

This study examines changes in gill Na(+),K(+)-ATPase (NKA) alpha- and beta-subunit isoforms, Na(+),K(+),2Cl(-) cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-alpha1b, -alpha3, -beta(1) and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-alpha1a mRNA decreased significantly in anadromous salmon from February through June, whereas alpha1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-alpha1b and NKCC mRNA increased in both strains, whereas NKA-alpha1a decreased. Both strains exhibited a transient increase in gill NKA alpha-protein abundance, with peak levels in May. Gill alpha-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-alpha1a, -alpha1b and -alpha3 isoforms may be important for potential functional differences in NKA, both during preparatory development and during salinity adjustments in salmon. Furthermore, landlocked salmon have lost some of the unique preparatory upregulation of gill NKA, NKCC and, to some extent, CFTR anion channel associated with the development of hypo-osmoregulatory ability in anadromous salmon.

Authors+Show Affiliations

Department of Biology, University of Bergen, High Technology Centre, Bergen N-5020, Norway. Tom.Nilsen@bio.uib.noNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17690237

Citation

Nilsen, Tom O., et al. "Differential Expression of Gill Na+,K+-ATPase Alpha- and Beta-subunits, Na+,K+,2Cl- Cotransporter and CFTR Anion Channel in Juvenile Anadromous and Landlocked Atlantic Salmon Salmo Salar." The Journal of Experimental Biology, vol. 210, no. Pt 16, 2007, pp. 2885-96.
Nilsen TO, Ebbesson LO, Madsen SS, et al. Differential expression of gill Na+,K+-ATPase alpha- and beta-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar. J Exp Biol. 2007;210(Pt 16):2885-96.
Nilsen, T. O., Ebbesson, L. O., Madsen, S. S., McCormick, S. D., Andersson, E., Björnsson, B. T., ... Stefansson, S. O. (2007). Differential expression of gill Na+,K+-ATPase alpha- and beta-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar. The Journal of Experimental Biology, 210(Pt 16), pp. 2885-96.
Nilsen TO, et al. Differential Expression of Gill Na+,K+-ATPase Alpha- and Beta-subunits, Na+,K+,2Cl- Cotransporter and CFTR Anion Channel in Juvenile Anadromous and Landlocked Atlantic Salmon Salmo Salar. J Exp Biol. 2007;210(Pt 16):2885-96. PubMed PMID: 17690237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential expression of gill Na+,K+-ATPase alpha- and beta-subunits, Na+,K+,2Cl- cotransporter and CFTR anion channel in juvenile anadromous and landlocked Atlantic salmon Salmo salar. AU - Nilsen,Tom O, AU - Ebbesson,Lars O E, AU - Madsen,Steffen S, AU - McCormick,Stephen D, AU - Andersson,Eva, AU - Björnsson,Björn Th, AU - Prunet,Patrick, AU - Stefansson,Sigurd O, PY - 2007/8/11/pubmed PY - 2007/11/2/medline PY - 2007/8/11/entrez SP - 2885 EP - 96 JF - The Journal of experimental biology JO - J. Exp. Biol. VL - 210 IS - Pt 16 N2 - This study examines changes in gill Na(+),K(+)-ATPase (NKA) alpha- and beta-subunit isoforms, Na(+),K(+),2Cl(-) cotransporter (NKCC) and cystic fibrosis transmembrane conductance regulator (CFTR I and II) in anadromous and landlocked strains of Atlantic salmon during parr-smolt transformation, and after seawater (SW) transfer in May/June. Gill NKA activity increased from February through April, May and June among both strains in freshwater (FW), with peak enzyme activity in the landlocked salmon being 50% below that of the anadromous fish in May and June. Gill NKA-alpha1b, -alpha3, -beta(1) and NKCC mRNA levels in anadromous salmon increased transiently, reaching peak levels in smolts in April/May, whereas no similar smolt-related upregulation of these transcripts occurred in juvenile landlocked salmon. Gill NKA-alpha1a mRNA decreased significantly in anadromous salmon from February through June, whereas alpha1a levels in landlocked salmon, after an initial decrease in April, remained significantly higher than those of the anadromous smolts in May and June. Following SW transfer, gill NKA-alpha1b and NKCC mRNA increased in both strains, whereas NKA-alpha1a decreased. Both strains exhibited a transient increase in gill NKA alpha-protein abundance, with peak levels in May. Gill alpha-protein abundance was lower in SW than corresponding FW values in June. Gill NKCC protein abundance increased transiently in anadromous fish, with peak levels in May, whereas a slight increase was observed in landlocked salmon in May, increasing to peak levels in June. Gill CFTR I mRNA levels increased significantly from February to April in both strains, followed by a slight, though not significant increase in May and June. CFTR I mRNA levels were significantly lower in landlocked than anadromous salmon in April/June. Gill CFTR II mRNA levels did not change significantly in either strain. Our findings demonstrates that differential expression of gill NKA-alpha1a, -alpha1b and -alpha3 isoforms may be important for potential functional differences in NKA, both during preparatory development and during salinity adjustments in salmon. Furthermore, landlocked salmon have lost some of the unique preparatory upregulation of gill NKA, NKCC and, to some extent, CFTR anion channel associated with the development of hypo-osmoregulatory ability in anadromous salmon. SN - 0022-0949 UR - https://www.unboundmedicine.com/medline/citation/17690237/Differential_expression_of_gill_Na+K+_ATPase_alpha__and_beta_subunits_Na+K+2Cl__cotransporter_and_CFTR_anion_channel_in_juvenile_anadromous_and_landlocked_Atlantic_salmon_Salmo_salar_ L2 - http://jeb.biologists.org/cgi/pmidlookup?view=long&pmid=17690237 DB - PRIME DP - Unbound Medicine ER -