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Divergence of transcription factor binding sites across related yeast species.
Science. 2007 Aug 10; 317(5839):815-9.Sci

Abstract

Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species.

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Authors+Show Affiliations

Borneman AR
Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06511, USA.
Gianoulis TA
No affiliation info available
Zhang ZD
No affiliation info available
Yu H
No affiliation info available
Rozowsky J
No affiliation info available
Seringhaus MR
No affiliation info available
Wang LY
No affiliation info available
Gerstein M
No affiliation info available
Snyder M
No affiliation info available

MeSH

Base SequenceBinding SitesCandida albicansChromatin ImmunoprecipitationDNA-Binding ProteinsEvolution, MolecularFungal ProteinsGene Expression Regulation, FungalGene Regulatory NetworksGenes, FungalOligonucleotide Array Sequence AnalysisRegulatory Sequences, Nucleic AcidSaccharomycesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription Factors

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17690298

Citation

Borneman, Anthony R., et al. "Divergence of Transcription Factor Binding Sites Across Related Yeast Species." Science (New York, N.Y.), vol. 317, no. 5839, 2007, pp. 815-9.
Borneman AR, Gianoulis TA, Zhang ZD, et al. Divergence of transcription factor binding sites across related yeast species. Science. 2007;317(5839):815-9.
Borneman, A. R., Gianoulis, T. A., Zhang, Z. D., Yu, H., Rozowsky, J., Seringhaus, M. R., Wang, L. Y., Gerstein, M., & Snyder, M. (2007). Divergence of transcription factor binding sites across related yeast species. Science (New York, N.Y.), 317(5839), 815-9.
Borneman AR, et al. Divergence of Transcription Factor Binding Sites Across Related Yeast Species. Science. 2007 Aug 10;317(5839):815-9. PubMed PMID: 17690298.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Divergence of transcription factor binding sites across related yeast species. AU - Borneman,Anthony R, AU - Gianoulis,Tara A, AU - Zhang,Zhengdong D, AU - Yu,Haiyuan, AU - Rozowsky,Joel, AU - Seringhaus,Michael R, AU - Wang,Lu Yong, AU - Gerstein,Mark, AU - Snyder,Michael, PY - 2007/8/11/pubmed PY - 2007/8/28/medline PY - 2007/8/11/entrez SP - 815 EP - 9 JF - Science (New York, N.Y.) JO - Science VL - 317 IS - 5839 N2 - Characterization of interspecies differences in gene regulation is crucial for understanding the molecular basis of both phenotypic diversity and evolution. By means of chromatin immunoprecipitation and DNA microarray analysis, the divergence in the binding sites of the pseudohyphal regulators Ste12 and Tec1 was determined in the yeasts Saccharomyces cerevisiae, S. mikatae, and S. bayanus under pseudohyphal conditions. We have shown that most of these sites have diverged across these species, far exceeding the interspecies variation in orthologous genes. A group of Ste12 targets was shown to be bound only in S. mikatae and S. bayanus under pseudohyphal conditions. Many of these genes are targets of Ste12 during mating in S. cerevisiae, indicating that specialization between the two pathways has occurred in this species. Transcription factor binding sites have therefore diverged substantially faster than ortholog content. Thus, gene regulation resulting from transcription factor binding is likely to be a major cause of divergence between related species. SN - 1095-9203 UR - https://www.unboundmedicine.com/medline/citation/17690298/Divergence_of_transcription_factor_binding_sites_across_related_yeast_species_ L2 - https://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=17690298 DB - PRIME DP - Unbound Medicine ER -