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Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve.
J Chem Neuroanat. 2008 Jan; 35(1):94-100.JC

Abstract

Single ligature nerve constriction (SLNC) is a newly developed animal model for the study of neuropathic pain. SLNC of the rat sciatic nerve induces pain-related behaviors, as well as changes in the expression of neuropeptide tyrosine and the Y(1) receptor in lumbar dorsal root ganglia (DRGs) and spinal cord. In the present study, we have analyzed the expression of another neuropeptide, galanin, in lumbar DRGs and spinal cord after different degrees of constriction of the rat sciatic nerve. The nerve was ligated and reduced to 10-30, 40-80 or 90% of its original diameter (light, medium or strong SLNCs). At different times after injury (7, 14, 30, 60 days), lumbar 4 and 5 DRGs and the corresponding levels of the spinal cord were dissected out and processed for galanin-immunohistochemistry. In DRGs, SLNC induced a gradual increase in the number of galanin-immunoreactive (IR) neurons, in direct correlation with the degree of constriction. Thus, after light SLNC, a modest upregulation of galanin was observed, mainly in small-sized neurons. However, following medium or strong SLNCs, there was a more drastic increase in the number of galanin-IR neurons, involving also medium and large-sized cells. The highest numbers of galanin-IR neurons were detected 14 days after injury. In the dorsal horn of the spinal cord, medium and strong SLNCs induced a marked ipsilateral increase in galanin-like immunoreactivity in laminae I-II. These results show that galanin expression in DRGs and spinal cord is differentially regulated by different degrees of nerve constriction and further support its modulatory role on neuropathic pain.

Authors+Show Affiliations

School of Biomedical Sciences, Austral University, Av. Pte. Perón 1500, B1629AHJ Pilar, Buenos Aires, Argentina. fcoronel@cas.austral.edu.arNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

17693056

Citation

Coronel, María Florencia, et al. "Differential Galanin Upregulation in Dorsal Root Ganglia and Spinal Cord After Graded Single Ligature Nerve Constriction of the Rat Sciatic Nerve." Journal of Chemical Neuroanatomy, vol. 35, no. 1, 2008, pp. 94-100.
Coronel MF, Brumovsky PR, Hökfelt T, et al. Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve. J Chem Neuroanat. 2008;35(1):94-100.
Coronel, M. F., Brumovsky, P. R., Hökfelt, T., & Villar, M. J. (2008). Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve. Journal of Chemical Neuroanatomy, 35(1), 94-100.
Coronel MF, et al. Differential Galanin Upregulation in Dorsal Root Ganglia and Spinal Cord After Graded Single Ligature Nerve Constriction of the Rat Sciatic Nerve. J Chem Neuroanat. 2008;35(1):94-100. PubMed PMID: 17693056.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential galanin upregulation in dorsal root ganglia and spinal cord after graded single ligature nerve constriction of the rat sciatic nerve. AU - Coronel,María Florencia, AU - Brumovsky,Pablo Rodolfo, AU - Hökfelt,Tomas, AU - Villar,Marcelo José, Y1 - 2007/07/19/ PY - 2007/05/11/received PY - 2007/07/11/revised PY - 2007/07/14/accepted PY - 2007/8/19/pubmed PY - 2008/4/23/medline PY - 2007/8/19/entrez SP - 94 EP - 100 JF - Journal of chemical neuroanatomy JO - J Chem Neuroanat VL - 35 IS - 1 N2 - Single ligature nerve constriction (SLNC) is a newly developed animal model for the study of neuropathic pain. SLNC of the rat sciatic nerve induces pain-related behaviors, as well as changes in the expression of neuropeptide tyrosine and the Y(1) receptor in lumbar dorsal root ganglia (DRGs) and spinal cord. In the present study, we have analyzed the expression of another neuropeptide, galanin, in lumbar DRGs and spinal cord after different degrees of constriction of the rat sciatic nerve. The nerve was ligated and reduced to 10-30, 40-80 or 90% of its original diameter (light, medium or strong SLNCs). At different times after injury (7, 14, 30, 60 days), lumbar 4 and 5 DRGs and the corresponding levels of the spinal cord were dissected out and processed for galanin-immunohistochemistry. In DRGs, SLNC induced a gradual increase in the number of galanin-immunoreactive (IR) neurons, in direct correlation with the degree of constriction. Thus, after light SLNC, a modest upregulation of galanin was observed, mainly in small-sized neurons. However, following medium or strong SLNCs, there was a more drastic increase in the number of galanin-IR neurons, involving also medium and large-sized cells. The highest numbers of galanin-IR neurons were detected 14 days after injury. In the dorsal horn of the spinal cord, medium and strong SLNCs induced a marked ipsilateral increase in galanin-like immunoreactivity in laminae I-II. These results show that galanin expression in DRGs and spinal cord is differentially regulated by different degrees of nerve constriction and further support its modulatory role on neuropathic pain. SN - 0891-0618 UR - https://www.unboundmedicine.com/medline/citation/17693056/Differential_galanin_upregulation_in_dorsal_root_ganglia_and_spinal_cord_after_graded_single_ligature_nerve_constriction_of_the_rat_sciatic_nerve_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-0618(07)00123-8 DB - PRIME DP - Unbound Medicine ER -